02033nas a2200193   4500008004100000022001400041245008100055210006900136260007100205300001400276490000600290520133200296653001601628653003501644653001501679100002001694700001701714856010801731       2014                        eng d  a1944-824400aControlling the cell adhesion property of silk films by graft polymerization0 aControlling the cell adhesion property of silk films by graft po  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcAPR  a5005-50110 v63 a<p>We report here a graft polymerization method to improve the cell adhesion property of Bombyx mori silk fibroin films. B. mori silk has evolved as a promising material for tissue engineering because of its biocompatibility and biodegradability. However, silk's hydrophobic character makes cell adhesion and proliferation difficult. Also, the lack of sufficient reactive amino acid residues makes biofunctionalization via chemical modification challenging. Our study describes a simple method that provides increased chemical handles for tuning of the surface chemistry of regenerated silk films (SFs), thus allowing manipulation of their bioactivity. By grafting pAAc and pHEMA via plasma etching, we have increased carboxylic acid and hydroxyl groups on silk, respectively. These modifications allowed us to tune the hydrophilicity of SFs and provide functional groups for bioconjugation. Our strategy also allowed us to develop silk-based surface coatings, where spatial control over cell adhesion can be achieved. This control over cell adhesion in a particular region of the SFs is difficult to obtain via existing methods of modifying the silk fibroin instead of the SF surface. Thus, our strategy will be a valuable addition to the toolkit of biofunctionalization for enhancing SFs' tissue engineering applications.</p>10aBombyx mori10apolymer grafting cell adhesion10asilk films1 aDhyani, Vartika1 aSingh, Neetu  uhttps://library.ncl.res.in/content/controlling-cell-adhesion-property-silk-films-graft-polymerization-0