02465nas a2200241   4500008004100000022001400041245008400055210006900139260008000208300001200288490000700300520158900307653002701896653001901923653002201942653001901964653001801983100002802001700002202029700002302051700003102074856011802105       2014                        eng d  a1097-395800aAntimicrobial and SEM studies of sophorolipids synthesized using lauryl alcohol0 aAntimicrobial and SEM studies of sophorolipids synthesized using  aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAY  a543-5520 v173 a<p>In recent years, researchers have developed biosurfactants for industrial, pharmaceutical and medical applications revealing the promising biological activities of these biomolecules. One of the best studied microbial surfactants are glycolipids, especially sophorolipids (SLs) produced by selected non-pathogenic yeast species of Candida. They are biodegradable, non-toxic and are environmentally friendly. Sophorolipid production was carried out using glucose as the hydrophilic source and lauryl alcohol C12-14, as the hydrophobic source using Candida bombicola ATCC 22214. Primary characterization of the SL obtained using lauryl alcohol (SLLA) was done by FTIR which depicted the presence of alkyl sophorosides/SLs. Antimicrobial activity testing revealed that SLLA showed complete inhibition against gram negative bacteria, Escherichia coli (ATCC 8739) Pseudomonas aeruginosa (ATCC 9027) at 30 and 1 mu g/ml at a contact time of 2 and 4 h respectively. Whereas for gram positive bacteria Staphylococcus aureus (ATCC 6358), Bacillus subtilis (ATCC 6633), complete inhibition was observed at 6 and 1 mu g/ml respectively at a contact time of 4 h. The formed SLLA showed noteworthy inhibition against the pathogenic yeast Candida albicans (ATCC 2091) at 50 mu g/ml with a contact time of 4 h. These values are remarkably low compared to reported values of oleic acid SLs and linolenic acid SLs which were studied for antimicrobial properties. Scanning electron microscopy analysis of the treated cells revealed the changes in morphology and topography of the microorganisms.</p>10aAntimicrobial property10aBiosurfactants10aCandida bombicola10aLauryl alcohol10asophorolipids1 aDengle-Pulate, Vrushali1 aChandorkar, Parul1 aBhagwat, Sunil, S.1 aPrabhune, Asmita, Ashutosh  uhttps://library.ncl.res.in/content/antimicrobial-and-sem-studies-sophorolipids-synthesized-using-lauryl-alcohol-0