01845nas a2200217   4500008004100000022001400041245010700055210006900162260007600231300001200307490000600319520104600325653002001371653001101391653001101402653001701413100002601430700001901456700002101475856013101496       2011                        eng d  a1860-676800aDifferentiation-inducing ability of sophorolipids of oleic and linoleic acids using a glioma cell line0 aDifferentiationinducing ability of sophorolipids of oleic and li  aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcMAY  a509-5120 v63 a<p>Sophorolipids are biosurfactants produced by non-pathogenic yeasts. They show structural similarity with the membrane components of mammalian cells, i.e., glycosphingolipids and gangliosides, which are involved in processes such as signaling, oncogenesis, and differentiation. Sophorolipids have been reported to induce differentiation in several leukemic cell lines, cell death via apoptosis in a human liver cancer cell line, and necrosis in a pancreatic adenocarcinoma cell line. Here we report, for the first time, the effects of precursor fatty acids and sophorolipids of oleic and linoleic acids in pure acidic and crude forms on LN-229, a glioma cell line. In response to different sophorolipid forms, various morphological changes were observed, such as formation of long thread-like extensions arising from the ends of the cells, cell alignment, cell elongation and bundle formation in a dose-dependent manner. In this study we present the morphological evidence of the potential of sophorolipids as differentiation inducers.</p>10aDifferentiation10aGlioma10aLN-22910aSophorolipid1 aJoshi-Navare, Kasturi1 aShiras, Anjali1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/differentiation-inducing-ability-sophorolipids-oleic-and-linoleic-acids-using-glioma-cell-002865nas a2200229   4500008004100000022001400041245010300055210006900158260006700227300001400294490000800308520201300316653002502329653003502354653002102389653001702410653001402427100002602441700002602467700002102493856012102514       2014                        eng d  a1438-769700aNew yeast isolate Pichia caribbica synthesizes xylolipid biosurfactant with enhanced functionality0 aNew yeast isolate Pichia caribbica synthesizes xylolipid biosurf  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcAUG  a1070-10790 v1163 a<p>Sophorolipids (SL) belong to a class of glycolipidic biosurfactants suitable for a wide range of applications. The structural diversity in SL gives rise to variation in physicochemical and biological properties. To achieve the less explored head group diversity in sophorolipid structure, a new xylose fermenting yeast Pichia caribbica has been employed for biosurfactant production. The media and fermentation parameters have been optimized to achieve maximum yield of 7.48 g/L. The physicochemical properties of the xylolipid biosurfactant have been assessed. It reduced the surface tension of distilled water from 70 to 35.9 mN/m with the low critical micellar concentration (CMC) 1.0 mg/L as compared to typical SL (reported CMC range 40-100 mg/L). Structural characterization was done using FTIR and HR-MS to identify the structure. 17-L-[(beta-D-xylopyranosyl)-oxy]-Delta 9-heptadecanoic acid correlated to m/z 415 majorly constituted the product. Control experiment was performed in which glucose was provided as the hydrophilic carbon. This product was also subjected to HR-MS analysis to determine its chemical nature and found to be different from xylolipid. Presence of xylose as head group was anticipated to give altered physicochemical and biological activities and indeed a low CMC value and better inhibitory action was demonstrated against Staphylococcus aureus. Practical applications: Sophorolipids produced by microbial sources have several advantageous properties over commercial petroleum-based surfactants including biodegradability, environmentally friendly nature, and low toxicity. Here, we have attempted to modify the hydrophilic head group by incorporating xylose in place of glucose. This molecule will also behave differently in response to different stimuli. Stimuli-responsive surfactants are a class of compounds with applications in stabilization of emulsions, suspensions or foams, drug encapsulation and delivery, hard-surface cleaning, personal care applications.</p>10aAntibacterial action10aCritical micelle concentration10aPichia caribbica10aSophorolipid10aXylolipid1 aJoshi-Navare, Kasturi1 aSingh, Pradeep, Kumar1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/new-yeast-isolate-pichia-caribbica-synthesizes-xylolipid-biosurfactant-enhanced-0