02947nas a2200241   4500008004100000022001200041245009300053210006900146260000900215300001200224490000800236520215500244653002402399653001902423653001602442653001702458653001302475100002502488700001602513700002602529700002302555856012702578       2018                        eng d  a302893300aUntapped bacterial diversity and metabolic potential within Unkeshwar hot springs, India0 aUntapped bacterial diversity and metabolic potential within Unke  cJUL   a753-7700 v2003 a<p>Hot springs support diverse and interesting groups of microorganisms adapted to extreme conditions and gaining attention in biotechnological applications. However, due to limitations of cultivation methods, a majority of such extremophiles remain uncultivated and unexplored. The advent of multiple cultivation conditions and specialized culture media could possibly aid to access the unexplored microbial portion of hot springs. In the present study, different media and isolation strategies were applied to isolate hitherto unexplored bacterial taxa in the water samples collected from Unkeshwar hot springs, India. Molecular, phylogenetic and predictive functional characterization of the isolated bacterial population was done using 16S rRNA sequencing coupled with Tax4Fun tools. Furthermore, representative isolates were screened for important enzymes (cellulase, xylanase, amylase, and protease) and heavy metal tolerance (chromium, arsenic) properties. A total of 454 bacterial isolates obtained were mapped into 57 unique bacterial genera and 4 different bacterial phyla. Interestingly, 37 genera not previously isolated from Indian hot springs, were isolated for the first time in the present study. However, most of these genera (23 out of 37) were reported only in metagenomics studies from Indian and global hot springs. Furthermore, around 14 genera not previously cultivated and not detected in metagenomics studies of hot springs are documented here. The metabolic potential was ascertained by determining the abundance of specific genes using in silico based Tax4Fun tool, which identified around 315 metabolic pathways for metabolism of carbohydrates, synthesis of secondary metabolites and degradation of xenobiotic compounds. Bioprospection study revealed that 33 and 25 bacterial genera were positive for enzyme production and resistance to the heavy metals, respectively. The present study revealed the advantages of cultivation methods using a comprehensive multiple isolation approach for exploring untapped and unique bacterial diversity, and also utilities for various biotechnological and environmental applications.</p>10aBacterial diversity10aBioprospecting10aCultivation10aHeavy metals10aUntapped1 aMehetre, Gajanan, T.1 aShah, Manan1 aDastager, Syed, Gulam1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/untapped-bacterial-diversity-and-metabolic-potential-within-unkeshwar-hot-springs-india