01917nas a2200229   4500008004100000022001400041245011600055210006900171260006700240300001200307490000800319520106400327653001801391653002801409653000801437100002201445700002501467700002301492700002801515700002501543856011901568       2014                        eng d  a0378-109700aIsolation of streptococcus tigurinus - a novel member of streptococcus mitis group from a case of periodontitis0 aIsolation of streptococcus tigurinus a novel member of streptoco  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcAUG  a131-1350 v3573 a<p>Streptococcus tigurinus is a new member of the Streptococcus viridians group and is closely related to Streptococcus mitis, Streptococcus pneumoniae, Streptococcus pseudopneumoniae, Streptococcus oralis, and Streptococcus infantis. The type strain AZ_3a(T) of S. tigurinus was originally isolated from a patient with infective endocarditis. Accurate identification of S. tigurinus is facilitated only by newer molecular methods like 16S rRNA gene analysis. During the course of study on bacteraemia and infective endocarditis with reference to periodontitis and viridians group of streptococci, a strain of S. tigurinus isolated from subgingival plaque of a patient with periodontitis identified by 16S rRNA gene analysis, which was originally identified as Streptococcus pluranimalium by Vitek 2. Confirmation by 16S rRNA gene analysis showed 99.39% similarity (1476/1485 bp) with S. tigurinus AZ_3a(T) (AORU01000002). To the best of our knowledge, this is the first report of isolation of S. tigurinus from the oral cavity of a periodontitis patient.</p>10aperiodontitis10aStreptococcus tigurinus10aVGS1 aDhotre, Shree, V.1 aMehetre, Gajanan, T.1 aDharne, Mahesh, S.1 aSuryawanshi, Namdev, M.1 aNagoba, Basavraj, S.  uhttps://library.ncl.res.in/content/isolation-streptococcus-tigurinus-novel-member-streptococcus-mitis-group-case-002138nas a2200169   4500008004100000245014300041210006900184260000800253300001200261490000600273520146200279100002501741700002401766700002601790700002301816856012901839       2016                        eng d00aComplete metagenome sequencing based bacterial diversity and functional insights from basaltic hot spring of Unkeshwar, Maharashtra, India0 aComplete metagenome sequencing based bacterial diversity and fun  cMAR  a140-1430 v73 a<p>Unkeshwar hot springs are located at geographical South East Deccan Continental basalt of India. Here, we report the microbial community analysis of this hot spring using whole metagenome shotgun sequencing approach. The analysis revealed a total of 848,096 reads with 212.87Mbps with 50.87% G+C content. Metagenomic sequences were deposited in SRA database with accession number (SUB1242219). Community analysis revealed 99.98% sequences belonging to bacteria and 0.01% to archaea and 0.01% to Viruses. The data obtained revealed 41 phyla including bacteria and Archaea and including 719 different species. In taxonomic analysis, the dominant phyla were found as, Actinobacteria (56%), Verrucomicrobia (24%), Bacteriodes (13%), Deinococcus-Thermus (3%) and firmicutes (2%) and Viruses (2%). Furthermore, functional annotation using pathway information revealed dynamic potential of hot spring community in terms of metabolism, environmental information processing, cellular processes and other important aspects. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of each contig sequence by assigning KEGG Orthology (KO) numbers revealed contig sequences that were assigned to metabolism, organismal system, Environmental Information Processing, cellular processes and human diseases with some unclassified sequences. The Unkeshwar hot springs offer rich phylogenetic diversity and metabolic potential for biotechnological applications.</p>1 aMehetre, Gajanan, T.1 aParanjpe, Aditi, S.1 aDastager, Syed, Gulam1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/complete-metagenome-sequencing-based-bacterial-diversity-and-functional-insights-basaltic01684nas a2200253   4500008004100000022001400041245011500055210006900170260007600239300001200315490000600327520077300333653001501106653001501121653002101136653001201157100001801169700002301187700001601210700002501226700002301251700002501274856013101299       2016                        eng d  a1876-034100aDetection of clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin0 aDetection of clostridium tetani in human clinical samples using   a84 THEOBALDS RD, LONDON WC1X 8RR, ENGLANDbELSEVIER SCIENCE LONDONcJUL  a105-1090 v93 a<p>Tetanus resulting from ear injury remains an important health problem, particularly in the developing world. We report the successful detection of Clostridium tetani using tetX specific primers targeting the Cl. tetani neurotoxin. The sample was obtained from an ear discharge of a case of otogenic tetanus in a 2-year-old male child. Based on the culture results of the ear discharge, Gram staining and virulence testing by genotyping, a diagnosis of tetanus was confirmed. This is the first report from India on the successful detection of Cl. tetani in a human clinical sample using tetX specific primers targeting the Cl. tetani neurotoxin. (C) 2015 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Limited. All rights reserved.</p>10aCl. tetani10aNeurotoxin10aOtogenic tetanus10aTrismus1 aGanesh, Madhu1 aSheikh, Nasira, K.1 aShah, Pooja1 aMehetre, Gajanan, T.1 aDharne, Mahesh, S.1 aNagoba, Basavraj, S.  uhttps://library.ncl.res.in/content/detection-clostridium-tetani-human-clinical-samples-using-tetx-specific-primers-targeting-001139nas a2200157   4500008004100000245013700041210006900178260000800247490000600255520049900261100002500760700002000785700002600805700002300831856012700854       2016                        eng d00aInvestigation of microbial diversity in geothermal hot springs in Unkeshwar, India, based on 16S rRNA amplicon metagenome sequencing0 aInvestigation of microbial diversity in geothermal hot springs i  cFEB0 v43 a<p>Microbial diversity in geothermal waters of the Unkeshwar hot springs in Maharashtra, India, was studied using 16S rRNA amplicon metagenomic sequencing. Taxonomic analysis revealed the presence of Bacteroidetes , Proteobacteria , Cyanobacteria , Actinobacteria , Archeae , and OD1 phyla. Metabolic function prediction analysis indicated a battery of biological information systems indicating rich and novel microbial diversity, with potential biotechnological applications in this niche.</p>1 aMehetre, Gajanan, T.1 aParanjpe, Aditi1 aDastager, Syed, Gulam1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/investigation-microbial-diversity-geothermal-hot-springs-unkeshwar-india-based-16s-rrna02947nas a2200241   4500008004100000022001200041245009300053210006900146260000900215300001200224490000800236520215500244653002402399653001902423653001602442653001702458653001302475100002502488700001602513700002602529700002302555856012702578       2018                        eng d  a302893300aUntapped bacterial diversity and metabolic potential within Unkeshwar hot springs, India0 aUntapped bacterial diversity and metabolic potential within Unke  cJUL   a753-7700 v2003 a<p>Hot springs support diverse and interesting groups of microorganisms adapted to extreme conditions and gaining attention in biotechnological applications. However, due to limitations of cultivation methods, a majority of such extremophiles remain uncultivated and unexplored. The advent of multiple cultivation conditions and specialized culture media could possibly aid to access the unexplored microbial portion of hot springs. In the present study, different media and isolation strategies were applied to isolate hitherto unexplored bacterial taxa in the water samples collected from Unkeshwar hot springs, India. Molecular, phylogenetic and predictive functional characterization of the isolated bacterial population was done using 16S rRNA sequencing coupled with Tax4Fun tools. Furthermore, representative isolates were screened for important enzymes (cellulase, xylanase, amylase, and protease) and heavy metal tolerance (chromium, arsenic) properties. A total of 454 bacterial isolates obtained were mapped into 57 unique bacterial genera and 4 different bacterial phyla. Interestingly, 37 genera not previously isolated from Indian hot springs, were isolated for the first time in the present study. However, most of these genera (23 out of 37) were reported only in metagenomics studies from Indian and global hot springs. Furthermore, around 14 genera not previously cultivated and not detected in metagenomics studies of hot springs are documented here. The metabolic potential was ascertained by determining the abundance of specific genes using in silico based Tax4Fun tool, which identified around 315 metabolic pathways for metabolism of carbohydrates, synthesis of secondary metabolites and degradation of xenobiotic compounds. Bioprospection study revealed that 33 and 25 bacterial genera were positive for enzyme production and resistance to the heavy metals, respectively. The present study revealed the advantages of cultivation methods using a comprehensive multiple isolation approach for exploring untapped and unique bacterial diversity, and also utilities for various biotechnological and environmental applications.</p>10aBacterial diversity10aBioprospecting10aCultivation10aHeavy metals10aUntapped1 aMehetre, Gajanan, T.1 aShah, Manan1 aDastager, Syed, Gulam1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/untapped-bacterial-diversity-and-metabolic-potential-within-unkeshwar-hot-springs-india05035nas a2200205   4500008004100000245016600041210006900207260000800276300001400284490000600298520424600304100002504550700001904575700002404594700001404618700002104632700002304653700002204676856013104698       2019                        eng d00aBioactivities and molecular networking-based elucidation of metabolites of potent actinobacterial strains isolated from the Unkeshwar geothermal springs in India0 aBioactivities and molecular networkingbased elucidation of metab  cMAR  a9850-98590 v93 a<p><span class="hitHilite">The</span> bioactive potential <span class="hitHilite">of</span> Actinobacteria endemic <span class="hitHilite">to</span> hot <span class="hitHilite">springs</span> has rarely been investigated. This study highlights <span class="hitHilite">the</span> cultivable diversity and <span class="hitHilite">bioactivities</span> <span class="hitHilite">of</span> Actinobacteria associated <span class="hitHilite">with</span> <span class="hitHilite">the</span> <span class="hitHilite">Unkeshwar</span> hot <span class="hitHilite">springs</span>, <span class="hitHilite">India</span>. <span class="hitHilite">Potent</span> <span class="hitHilite">strains</span> were evaluated for their biosynthetic potentials and metabolite analysis was performed using effective dereplication <span class="hitHilite">molecular</span> networking tools. <span class="hitHilite">A</span> total <span class="hitHilite">of</span> 86 <span class="hitHilite">actinobacterial</span> <span class="hitHilite">strains</span> were <span class="hitHilite">isolated</span> and grouped <span class="hitHilite">into</span> 21 distinct genera, based <span class="hitHilite">on</span> 16S rRNA gene sequence analysis. These <span class="hitHilite">strains</span> included rare members such as Micromonospora, Marmoricola, Actinomadura, Cellulomonas, Cellulosimicrobium, Janibacter, Rothia, Barrentisimonas, Dietzia and Glycomyces. <span class="hitHilite">In</span> antimicrobial screening, Micromonospora sp. strain GH99 and Streptomyces sp. strain GH176 were found <span class="hitHilite">to</span> be <span class="hitHilite">potent</span> antimicrobial <span class="hitHilite">strains</span>. <span class="hitHilite">The</span> metabolic extracts <span class="hitHilite">of</span> these <span class="hitHilite">strains</span> exhibited strong antimicrobial activity against Staphylococcus epidermidis (NCIM 2493), Shigella flexneri (NCIM 5265), Klebsiella pneumonia (NCIM 2098), and Salmonella abony (NCIM 2257). <span class="hitHilite">The</span> extracts also displayed strong anti-biofilm and anticancer <span class="hitHilite">activities</span> against Pseudomonas aeruginosa (NCIM 5029), Acinetobacter junii (NCIM 5188) and breast cancer cell line MCF7, respectively. Both <span class="hitHilite">strains</span> also tested positive for <span class="hitHilite">the</span> presence <span class="hitHilite">of</span> <span class="hitHilite">the</span> PKS biosynthetic gene cluster <span class="hitHilite">in</span> their genomes. <span class="hitHilite">To</span> effectively delineate <span class="hitHilite">the</span> secondary <span class="hitHilite">metabolites</span>, <span class="hitHilite">the</span> extracts were subjected <span class="hitHilite">to</span> MS/MS-guided <span class="hitHilite">molecular</span> networking analysis. Structurally diverse compounds including <span class="hitHilite">the</span> polyketides 22-dehydroxymethyl-kijanolide (GH99 strain) and Abyssomicin I (GH176 strain) were detected <span class="hitHilite">in</span> <span class="hitHilite">the</span> extracts. Interestingly, Brevianamide F was detected <span class="hitHilite">in</span> <span class="hitHilite">the</span> extract <span class="hitHilite">of</span> Micromonospora, which has previously been mostly found <span class="hitHilite">in</span> fungal species. Other compounds such as cyclic tripeptides, Cyclo(l-Pro-d-Ile) and Cyclo(d-Pro-l-Phe), were also identified <span class="hitHilite">in</span> this strain. <span class="hitHilite">In</span> summary, for <span class="hitHilite">the</span> first time, we explored <span class="hitHilite">the</span> diversity <span class="hitHilite">of</span> Actinobacteria and evaluated their bioactive potential <span class="hitHilite">from</span> <span class="hitHilite">the</span> <span class="hitHilite">Unkeshwar</span> hot <span class="hitHilite">springs</span>. <span class="hitHilite">The</span> <span class="hitHilite">potent</span> <span class="hitHilite">strains</span> <span class="hitHilite">isolated</span> <span class="hitHilite">in</span> <span class="hitHilite">the</span> study could be useful <span class="hitHilite">in</span> drug <span class="hitHilite">discovery</span> programs.</p>
1 aMehetre, Gajanan, T.1 aVinodh, J., S.1 aBurkul, Bhushan, B.1 aDesai, D.1 aSanthakumari, B.1 aDharne, Mahesh, S.1 aDastager, Syed, G  uhttps://library.ncl.res.in/content/bioactivities-and-molecular-networking-based-elucidation-metabolites-potent-actinobacterial02891nas a2200241   4500008004100000022001400041245015700055210006900212260000800281300001000289490000800299520204400307653001902351653001902370653001402389653001802403653000902421653001702430100002502447700002302472700002302495856013102518       2019                        eng d  a0048-969700aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure and mixed cultures of biosurfactant producing thermophilic and thermo-tolerant bacteria0 aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure  cAUG  a52-600 v6793 a<p>Applicability of thermophilic and thereto-tolerant microorganisms for biodegradation of polycyclic aromatic hydrocarbons (PAHs) with low water solubility is an interesting strategy for improving the biodegradation efficiency. In this study, we evaluated utility of thermophilic and thermo-tolerant bacteria isolated from Unkeshwar hot spring (India) for biodegradation of four different PAHs. Water samples were enriched in mineral salt medium (MSM) containing a mixture of four PAHs compounds (anthracene: ANT, fluorene: FLU, phenanthrenc: PHE and pyrene: PYR) at 37 degrees C and 50 degrees C. After growth based screening, four potent strains obtained which were identified as Aeribacillus pallidus (UCPS2), Bacillus axarquiensis (UCPD1), Bacillus siamensis (GHP76) and Bacillus subtilis subsp. inaquosorum (U277) based on the 16S rRNA gene sequence analysis. Degradation of mixed PAH compounds was evaluated by pure as well as mixed cultures under shake flask conditions using MSM supplemented with 200 mg/L concentration of PAHs (50 mg/L of each compound) for 15 days at 37 degrees C and 50 degrees C. A relatively higher degradation of ANT (92% - 96%), FLU (83% - 86%), PHE (16% - 54%) and PYR (51% - 71%) was achieved at 50 degrees C by Aeribacillus sp. (UCPS2) and mixed culture. Furthermore, crude oil was used as a substrate to study the degradation of same PAHs using these organisms which also revealed with similar results with the higher degradation at 50 degrees C. Interestingly, PAH-degrading strains were also positive for biosurfactant production. Biosurfactants were identified as the variants of surfactins (lipopeptide biosurfactants) based on analytical tools and phylogenetic analysis of the surfactin genes. Overall, this study has shown that hot spring microbes may have a potential for PAHs degradation and also biosurfactant production at a higher temperature, which could provide a novel perspective for removal of PAHs residues from oil contaminated sites. (C) 2019 Elsevier B.V. All rights reserved.</p>
10aBiodegradation10aBiosurfactants10aCrude oil10aMixed culture10aPAHs10aThermophiles1 aMehetre, Gajanan, T.1 aDastager, Syed, G.1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/biodegradation-mixed-polycyclic-aromatic-hydrocarbons-pure-and-mixed-cultures-biosurfactant