01250nas a2200205   4500008004100000022001400041245009300055210006900148260007100217300001400288490000800302520048800310100002100798700002400819700001100843700002400854700001900878700002600897856012100923       2012                        eng d  a0002-786300aHighly efficient (R-X-R)-type carbamates as molecular transporters for cellular delivery0 aHighly efficient RXRtype carbamates as molecular transporters fo  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAY  a7196-71990 v1343 a<p>The (R-X-R) motif-containing arginine-rich peptides are among the most effective cell-penetrating peptides. The replacement of amide linkages in the (R-X-R) motif by carbamate linkages as in (r-ahx-r)(4) or (r-ahx-r-r-apr-r)(2) increases the efficacy of such oligomers several-fold. Internalization of these oligomers in mammalian cell lines occurs by an energy-independent process. These oligomers show efficient delivery of biologically active plasmid DNA into CHO-K1 cells.</p>1 aPatil, Kiran, M.1 aNaik, Rangeetha, J.1 aRajpal1 aFernandes, Moneesha1 aGanguli, Munia1 aKumar, Vaijayanti, A.  uhttps://library.ncl.res.in/content/highly-efficient-r-x-r-type-carbamates-molecular-transporters-cellular-delivery-001962nas a2200277   4500008004100000022001400041245018700055210006900242260010600311300001400417490000700431520083800438653001801276653003001294653002901324653003401353653002001387100002101407700002401428700001601452700002001468700002601488700001901514700002401533856012701557       2014                        eng d  a0960-894X00aSecond generation, arginine-rich (R-X `-R)(4)-type cell-penetrating alpha-omega-alpha-peptides with constrained, chiral omega-amino acids (X `) for enhanced cargo delivery into cells0 aSecond generation argininerich RX R4type cellpenetrating alphaom  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcSEP  a4198-42020 v243 a<p>The syntheses of novel N-aminoalkyl proline-derived spacers (X') in polycationic (R-X'-R)-motif cell-penetrating alpha-omega-alpha-peptides are described as improved molecular transporters and their structural features studied by CD. FACS analysis shows enhanced cellular uptake and confocal microscopy indicates predominantly cytoplasmic localization. The oligomers are efficient at transporting pDNA into cells. The chirality together with the hydrophobicity and flexibility derived from the spacer chain are found to have marked influence on the cell-penetrating and cargo delivery properties of the cell-penetrating peptides (CPPs). The peptides containing N-(3-aminopropyl)-D-proline spacers are found to be the best at cell penetration and cargo delivery in the present study. (C) 2014 Elsevier Ltd. All rights reserved.</p>10a(R-X-R)-motif10aalpha-omega-alpha-Peptide10aCell-penetrating peptide10aConstrained chiral amino acid10aPeptidomimetics1 aPatil, Kiran, M.1 aNaik, Rangeetha, J.1 aVij, Manika1 aYadav, Amit, K.1 aKumar, Vaijayanti, A.1 aGanguli, Munia1 aFernandes, Moneesha  uhttps://library.ncl.res.in/content/second-generation-arginine-rich-r-x-r4-type-cell-penetrating-alpha-omega-alpha-peptides