02081nas a2200169   4500008004100000022001400041245017800055210006900233260011000302300001600412490000600428520128300434100002001717700002401737700002601761856012401787       2016                        eng d  a2046-206900aPhotoresponsive assemblies of linear-dendritic copolymers containing azobenzene in the dendron interior: the effect of the dendron structure on dye encapsulation and release0 aPhotoresponsive assemblies of lineardendritic copolymers contain  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAPR  a43163-431700 v63 a<p>Hydrophobic dendrons with different numbers and positions of azobenzenes as well as different groups benzyl and dodecyl, on the periphery were synthesised and attached to poly(ethylene glycol) using copper-catalysed azide-alkyne cycloaddition to obtain linear-dendritic copolymers. Self-assembly of the polymers in aqueous solution was characterised using dynamic light scattering (DLS), transmission electron microscopy (TEM) and critical micelle concentration (cmc). Formation of H-aggregates during micellisation was shown for polymers with a higher number of azobenzene units. Photoisomerisation of azobenzene in the assemblies was studied and the rate constant of thermal photoisomerisation was calculated. Release of hydrophobic dye Nile red upon photoisomerisation of azobenzene occurred without disruption of micellar aggregates. Dye release varied with the pathway - thermal or visible light irradiation, followed for cis-trans isomerisation. The encapsulation capacity of the micelles and extent of dye release in either pathway were found to be influenced by the dendron structure. A polymer with a lower number of azobenzenes and aliphatic periphery on the dendron showed significantly different behaviour than polymers with a larger number of aromatic units.</p>1 aKalva, Nagendra1 aBasutkar, Nitin, B.1 aAmbade, Ashootosh, V.  uhttps://library.ncl.res.in/content/photoresponsive-assemblies-linear-dendritic-copolymers-containing-azobenzene-dendron02223nas a2200241   4500008004100000245016000041210006900201260000800270300001100278490000800289520133700297653002101634653002301655653001801678653001601696653001701712653002301729100002901752700002601781700002601807700002601833856012201859       2023                        eng d00aPermeable polymersomes from temperature and pH dual stimuli-responsive PVCL-b-PLL block copolymers for enhanced cell internalization and lysosome targeting0 aPermeable polymersomes from temperature and pH dual stimulirespo  cAUG  a2134540 v1513 a<p>
	A series of dual stimuli-responsive block copolymers comprising temperature-responsive poly(N-vinyl-caprolactam) (PVCL) and biodegradable pH-responsive poly(L-lysine) (PLL) of varying chain length were syn-thesized by a combination of free radical polymerization and ring opening polymerization. The block copolymers formed micelles and vesicles (polymersomes) in response to temperature and pH, respectively, in aqueous so-lution. The nanoassemblies were characterized by transmission electron microscopy and dynamic light scattering techniques. Encapsulation of both hydrophobic and hydrophilic dyes in the polymersomes was shown. Doxo-rubicin (DOX) was loaded in the polymersomes and its controlled release in response to the two stimuli, inde-pendently and jointly, was studied. The drug was found to be released due to stimuli-induced increased permeability without disassembly of the polymersomes. A significant increase in the cellular uptake of the drug-loaded polymersomes at hyperthermia conditions was demonstrated at 41 degrees C and release of the drug upon localization in lysosomes was observed. Cellular internalization pathway of the polymersomes was investigated by competitive inhibition assay and a combination of endocytic pathways dominated by caveolae-mediated mechanism was found to be operative.</p>
10ablock copolymers10aControlled release10adrug delivery10aendocytosis10aPolymersomes10aStimuli-responsive1 aSurapaneni, Sai, Geetika1 aChoudhari, Shakeb, N.1 aAvhad, Shankarrao, V.1 aAmbade, Ashootosh, V.  uhttps://library.ncl.res.in/content/permeable-polymersomes-temperature-and-ph-dual-stimuli-responsive-pvcl-b-pll-block