01994nas a2200169   4500008004100000022001400041245009700055210006900152260011000221300001400331490000600345520127700351100002001628700002001648700002601668856013001694       2015                        eng d  a1759-995400aControlled micellar disassembly of photo- and pH-cleavable linear-dendritic block copolymers0 aControlled micellar disassembly of photo and pHcleavable lineard  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAUG  a6826-68350 v63 a<p>A linear-dendritic block copolymer with both photo-and pH-cleavable linkages at the junction between the hydrophobic dendron and hydrophilic linear polymer was synthesised. The photocleavable o-nitrobenzyl group attached to alkyne was introduced at the focal point of polyester dendron and acid-degradable acetal linkage with terminal azide was introduced at one end of linear polyethylene glycol. The two segments were connected using click chemistry. The copolymer was found to self-assemble into micelle-like aggregates in dilute aqueous solution. Stimuli-responsive disassembly of the aggregates was studied by monitoring fluorescence emission of the encapsulated hydrophobic dye and dynamic light scattering (DLS). Products of acetal cleavage at acidic pH were analyzed by gel permeation chromatography and NMR spectroscopy. The photo and pH stimuli were applied separately as well as simultaneously to study the rate of dye release. The synergistic effect of the two stimuli on dye release was demonstrated and the non-cytotoxic nature of the polymer was shown using the MTT assay. Cell uptake of DOX-loaded micelles and photo-induced release of the drug resulting in significantly higher cytotoxicity than by free DOX was demonstrated using MDA-MB-231 cells.</p>1 aKalva, Nagendra1 aParekh, Nimisha1 aAmbade, Ashootosh, V.  uhttps://library.ncl.res.in/content/controlled-micellar-disassembly-photo-and-ph-cleavable-linear-dendritic-block-copolymers-001631nas a2200181   4500008004100000022001400041245010800055210006900163260007100232300001200303490000600315520092400321100001601245700002001261700002001281700002201301856012601323       2016                        eng d  a2161-165300aControlled synthesis of end-functionalized mannose-6-phosphate glycopolypeptides for lysosome targeting0 aControlled synthesis of endfunctionalized mannose6phosphate glyc  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcJUL  a809-8130 v53 a<p>The ubiquitous expression of the mannose-6-phosphate receptor on the majority of human cells makes it a valid target in the quest to deliver therapeutics selectively to the lysosome. In this work end-functionalized polyvalent mannose-6-phosphate glycopolypeptides (M6P-GPs) with high molecular weights (up to 22 kDa) have been synthesized via NCA polymerization. These synthetic M6P-GPs were found to display minimal toxicity to cells in vitro and show exceptional selectivity for trafficking into lysosomes in various cell lines. Comparison of the cellular uptake behavior of M6P-GP and the corresponding mannose-GP polymer reveals that incorporation of the phosphate moiety at the 6-position of mannose completely alters its trafficking behavior and becomes exclusively lysosome specific. We also demonstrate that trafficking of M6P-GPs in mammalian cells is likely associated with the CI-MPR receptor pathway.</p>1 aDas, Soumen1 aParekh, Nimisha1 aMondal, Basudeb1 aGupta, Sayam, Sen  uhttps://library.ncl.res.in/content/controlled-synthesis-end-functionalized-mannose-6-phosphate-glycopolypeptides-lysosome