02358nas a2200301 4500008004100000022001400041245018500055210006900240260006700309300001200376490000700388520119500395653002501590653002501615653003501640653001901675653001201694100003101706700002401737700002901761700002001790700002401810700001901834700001901853700002501872700003001897856012901927 2014 eng d a8756-793800aProtein-coated polymer as a matrix for enzyme immobilization: immobilization of trypsin on bovine serum albumin-coated allyl glycidyl ether-ethylene glycol dimethacrylate copolymer0 aProteincoated polymer as a matrix for enzyme immobilization immo a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcMAR a317-3230 v303 a

Allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) shows excellent bovine serum albumin (BSA) adsorption (up to 16% (w/w)) at pH 8.0 and the adsorbed BSA is strongly bound. This protein-coated polymer provides a novel matrix with naturally existing functional groups such as thiol, amino, and carboxylic acid that are available for covalent immobilization of functional enzymes. Employing appropriate strategies, trypsin as a model protein was covalently bound to BSA-coated matrix both independently, and in a stepwise manner on the same matrix, with less than 5% loss of enzyme activity during immobilization. Glutaraldehyde crosslinking after immobilization provide stable enzyme preparation with activity of 510 units/g recycled up to six times without loss of enzyme activity. AFM studies reveal that the polymer surface has protein peaks and valleys rather than a uniform monolayer distribution of the protein and the immobilized enzyme preparation can best be described as polymer supported cross-linked enzyme aggregates (CLEAs). (c) 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:317-323, 2014

10aallyl glycidyl ether10aBovine serum albumin10aethylene glycol dimethacrylate10aImmobilization10aTrypsin1 aJasti, Lakshmi, Swarnalath1 aDola, Sandhya, Rani1 aKumaraguru, Thenkrishnan1 aBajja, Sreedhar1 aFadnavis, Nitin, W.1 aAddepally, Uma1 aRajdeo, Kishor1 aPonrathnam, Surendra1 aDeokar, Sarika, Babasaheb uhttps://library.ncl.res.in/content/protein-coated-polymer-matrix-enzyme-immobilization-immobilization-trypsin-bovine-serum-001635nas a2200265 4500008004100000022001400041245010100055210006900156260007300225300001000298490000800308520069500316653001401011653001301025653002501038653001501063653001401078653002201092100002901114700002701143700002401170700001901194700002401213856013201237 2014 eng d a0926-860X00aRegioselective acylation of 2-methoxy naphthalene catalyzed by supported 12-phosphotungstic acid0 aRegioselective acylation of 2methoxy naphthalene catalyzed by su aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcSEP a55-610 v4863 a

12-Phosphotungstic acid supported on silica gel, zirconium sulfate, and a combination of silica gel and zirconium sulfate (50% w/w) were employed as solid acid catalysts for regioselective acylation of 2-methoxynaphtalene with acetic anhydride. 1-(6-Methoxynaphthalen-2-yl)ethanone (2,6-AMN), a commercially important intermediate for production of Naproxen, was obtained with excellent selectivity (>98%) at 67-68% conversion using 12-phosphotungstic acid supported on silica gel 20% (w/w) in refluxing tetrachloroethane. The unreacted starting material can be easily separated from the product by a simple crystallization from nonane. (C) 2014 Elsevier B.V. All rights reserved.

10aAcylation10aNaproxen10aPhosphotungstic acid10aSilica gel10aSupported10aZirconium sulfate1 aKumaraguru, Thenkrishnan1 aDevi, Ayala, Vedamayee1 aSiddaiah, Vidavalur1 aRajdeo, Kishor1 aFadnavis, Nitin, W. uhttps://library.ncl.res.in/content/regioselective-acylation-2-methoxy-naphthalene-catalyzed-supported-12-phosphotungstic-acid-002180nas a2200253 4500008004100000022001400041245009300055210006900148260009500217300001000312490000700322520129400329653001601623653002101639653001901660653001401679653002201693653001201715100001901727700002301746700001801769700002401787856011501811 2016 eng d a0960-308500aImmobilization of pectinase on reusable polymer support for clarification of apple juice0 aImmobilization of pectinase on reusable polymer support for clar a165-189 RAILWAY TERRACE, DAVIS BLDG, RUGBY CV21 3HQ, ENGLANDbINST CHEMICAL ENGINEERScJUL a12-190 v993 a

Pectinase (E.C.3.2.1.15) was successfully immobilized on recyclable polymer matrix. The immobilization matrix was prepared by reaction of polyethyleneimine (mol. wt. 70,000) with epoxy-activated acrylate copolymer DILBEAD-VWR. The enzyme pectinase was first adsorbed on the polymer at pH 7.0 via ion exchange and then stabilized by crosslinking with dextran aldehyde. While the free enzyme shows a pH-optimum of 5.0, the immobilized enzyme exhibited high level of activity in a broad pH range of pH 3.0-7.0. Although the thermal stability of free and immobilized enzymes was similar, at room temperature, the immobilized enzyme could be recycled more than 10 times with loss of less than 5% of its activity during clarification of apple juice. On the eventual loss of enzyme activity, the immobilized enzyme and dextran aldehyde can be easily removed from the polymer by a simple treatment with 1N HCl and the polymer can be reused for immobilization of a fresh batch of enzyme. This support also can be reused several times, making the process economically attractive. The properties of apple juice treated with immobilized enzyme were similar to those of that treated with free pectinase. (C) 2016 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

10aApple juice10aDextran aldehyde10aImmobilization10aPectinase10aPolyethyleneimine10aPolymer1 aRajdeo, Kishor1 aHarini, Tirunagari1 aLavanya, Kuna1 aFadnavis, Nitin, W. uhttps://library.ncl.res.in/content/immobilization-pectinase-reusable-polymer-support-clarification-apple-juice05855nas a2200301 4500008004100000022001400041245011100055210006900166260000800235300001600243490000700259520486100266653003905127653001805166653002405184653001505208653002705223100001905250700001605269700001805285700002005303700001905323700001905342700002505361700001905386700002405405856012405429 2023 eng d a0170-083900aNovel shear thickening fluids possessing high shear rates using monodispersed silica nanoparticles and PEG0 aNovel shear thickening fluids possessing high shear rates using  cDEC a13069-130980 v803 a

Higher shear rate possessing homogeneous shear thickening fluids were synthesized using monodispersed silica nanoparticles and polyethylene glycol (PEG). Novel homogeneous methodology was developed for the synthesis of shear thickening fluids using monodispersed silica nanoparticles and PEG. Shear rate of shear thickening fluids (STF) was determined using rheometer at room temperature. The normal shear rate was observed in the range of 100 to 1200 s(-1), whereas higher shear rate 2000 s(-1) was obtained using monodispersed silica nanoparticles, PEG and shear rate enhancer. Monodispersed silica nanoparticles were synthesized in a typical one-pot using modified Stober's method at room temperature. Precursors were used for the synthesis of monodispersed silica nanoparticles such as tetraethyl orthosilicate (TEOS), ethanol, deionized water and ammonia as catalyst. Particle size of monodispersed silica nanoparticles was in the range of 100-1200 nm, and particle size distribution was varied from 0.000 to 0.221. Synthesis of monodispersed silica nanoparticles was studied by various parameters, namely effect of TEOS concentration, grade of ethyl alcohol, ammonia concentration and water concentration. TEOS concentration effect shows particle size and particle size distribution increases with increase in concentration of TEOS. Even though concentration of TEOS was increased up to 5 times the observation was same for all particle sizes such as 100, 300 and 500 nm. Particularly in the case of 300 nm monodispersed silica nanoparticle synthesis, as TEOS concentration was increased from 1 to 5X (5 times) the particle size was increased from 331.7, 447.8, 497.0, 512.0 and 531.7 nm and particle size distribution was 0.005, 0.000, 0.006, 0.007 and 0.089, respectively. The effect of grade of ethyl alcohol illustrates that Indian rectified spirit shows almost similar results with respect to China make ethyl alcohol. Comparative study of China and Indian rectified spirit shows silica nanoparticle size was 174.6 and 174.2 nm, and particle size distribution was 0.065 and 0.071, respectively. Ammonia concentration effect explains particle size and particle size distribution increases with increase in concentration of ammonia. Water concentration effect shows particle size and particle size distribution increases with increase in concentration of ammonia. Comparative data of water concentration effect for 1316 and 1974 mL shows particle size were 321.7 and 488.0 nm, and particle size distribution was 0.083 and 0.05, respectively, under similar conditions. Morphological studies displayed the shape of the silica nanoparticles was spherical, monodispersed and isolated. A typical TEM image of monodispersed silica nanoparticles for 500 nm was observed with extremely low polydispersity, i.e., 0.000. It is very difficult to get such a type of excellent photograph of monodispersity by Stober's method particularly on large scale. Rheology study of homogeneous shear thickening fluids studied by varying the parameters such as composition of silica nanoparticles with PEG, effect of amount of solvent, effect of refluxing time, effect of additive with respect to the mol. wt. of PEG and effect of additive such as Tannin. All these parameters of STF explored with respect to the shear rate and shear viscosity. Effect of ethanol amount on STF demonstrates the shear rate increases with increase in amount of solvent. Effect of refluxing time on STF exhibits the shear rate increases with increase in refluxing time. Effect of composition based on 500 nm monodispersed silica nanoparticles and PEG 200 with respect to 60:40, 65:35 and 70:30 composition was studied and the results predict that shear rate increases with increase in silica percentage in composition, i.e., 118, 265 and 1200 s(-1) for 60:40, 65:35 and 70:30 composition, respectively, whereas maximum viscosity decreases with increase in silica content, i.e., 2.559, 1.420 and 1.200 Pa.s for 60:40, 65:35 and 70:30 composition, respectively. Effect of additive on shear rate and shear viscosity illustrates that shear rate decreases with increase in additive percentage in composition, whereas maximum viscosity increases with increase in additive content. Effect of additives on shear rate and shear viscosity was performed using 65:35 composition with respect to PEG 200 along with PEG 6000 shows shear rate was decreased from 142 to 6.42 s(-1) and maximum viscosity was increased from 56.1 to 571.0 Pa.s when the quantity of PEG 6000 was doubled. In general, additive decreases shear rate and increases shear viscosity (maximum). But effect of Tannin as an additive on 500 nm monodispersed silica nanoparticles with PEG 200 using 70:30 composition showed enormous increase in shear rate, i.e., 2000 s(-1). This is anomalous behavior of additive was observed.

10aMonodispersed silica nanoparticles10aNanocomposite10aPolyethylene glycol10aShear rate10aShear thickening fluid1 aChavan, Nayaku1 aDhage, Atul1 aWale, Apparav1 aThorave, Asmita1 aRajdeo, Kishor1 aKamble, Sanjay1 aPonrathnam, Surendra1 aTambe, Sanjeev1 aVerma, Sanjeevkumar uhttps://library.ncl.res.in/content/novel-shear-thickening-fluids-possessing-high-shear-rates-using-monodispersed-silica