02020nas a2200265   4500008004100000022001400041245010300055210006900158260007300227300001200300490000800312520108200320653001501402653001701417653001901434653001201453653001201465100003101477700002401508700001901532700001901551700003001570700002501600856012901625       2014                        eng d  a0927-776500aComparison of polymer induced and solvent induced trypsin denaturation: the role of hydrophobicity0 aComparison of polymer induced and solvent induced trypsin denatu  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAPR  a201-2050 v1163 a<p>Trypsin adsorption from aqueous buffer by various copolymers of allyl glycidyl ether-ethylene glycol dimethacrylate (AGE-EGDM) copolymer with varying crosslink density increases with increasing crosslink density and the effect slowly wears off after reaching a plateau at 50% crosslink density. The copolymer with 25% crosslink density was reacted with different amines with alkyl/aryl side chains to obtain a series of copolymers with 1,2-amino alcohol functional groups and varying hydrophobicity. Trypsin binding capacity again increases with hydrophobicity of the reacting amine and a good correlation between logP(octanol) of the amine and protein binding is observed. The bound trypsin is denatured to the extent of 90% in spite of the presence of hydrophilic hydroxyl and amino groups. The behavior was comparable to that in mixtures of aqueous buffer and water-miscible organic co-solvents where the solvent concentration required to deactivate 50% of the enzyme (C-50) is dependent on logP(octanol) of the co-solvent. (C) 2014 Elsevier B.V. All rights reserved.</p>10aAdsorption10aDenaturation10aHydrophobicity10aPolymer10aTrypsin1 aJasti, Lakshmi, Swarnalath1 aFadnavis, Nitin, W.1 aAddepally, Uma1 aDaniels, Siona1 aDeokar, Sarika, Babasaheb1 aPonrathnam, Surendra  uhttps://library.ncl.res.in/content/comparison-polymer-induced-and-solvent-induced-trypsin-denaturation-role-hydrophobicity-0