01773nas a2200229 4500008004100000022001400041245014100055210006900196260007500265300001200340490000700352520086000359653002401219653004301243653001201286653003401298653002201332100001901354700002401373700002601397856012001423 2013 eng d a0006-292800aCloning and sequence analysis of three variants of the gene encoding alkaline xylanase C from the alkaliphilic bacillus sp (NCL 87-6-10)0 aCloning and sequence analysis of three variants of the gene enco a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGER/PLENUM PUBLISHERScOCT a737-7490 v513 a

Alkaline xylanase C from the alkaliphilic Bacillus sp. (NCL 87-6-10) has a low molecular weight and alkaline pI and is cellulase-free, properties compatible with its use in the prebleaching of pulp. We report here the cloning and sequence analysis of three variants of the gene encoding xylanase C; xyl C1, xyl C2, and xyl C3. In phylogenetic analysis, the three xylanase C variants clustered into a single group along with other reported alkaline xylanases. Residues contributing to the alkaline pH were present in all three variants. DNA and protein sequence comparison of these variants with other reported alkaline xylanases revealed silent mutations, some of which are due to codon preference in the respective organisms. The recombinant Xyl C1 that was successfully expressed in E. coli BL21 (DE3) had properties similar to the native enzyme.

10aAlkaline xylanase C10aAlkaliphilic Bacillus sp (NCL 87-6-10)10aCloning10aE. coli BL21 (DE3) expression10aSequence analysis1 aSharma, Poonam1 aRele, Meenakshi, V.1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/cloning-and-sequence-analysis-three-variants-gene-encoding-alkaline-xylanase-c-001861nas a2200229 4500008004100000022001400041245014200055210006900197260007400266300001000340490000800350520093200358653002901290653002401319653002801343653002601371653003501397100002101432700002601453700002601479856012601505 2015 eng d a0016-670700aMale biased gene flow in banana pseudostem weevil (Odoiporus longicollis Oliver) as revealed by analysis of the COI-tRNA(Leu) COII region0 aMale biased gene flow in banana pseudostem weevil Odoiporus long aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a85-920 v1433 a

The genetic diversity amongst thirty weevils representing six Indian populations of banana pseudostem weevil i.e. Odoiporus longicollis (Oliver) was estimated by sequence analysis of the partial COI-tRNA(Leu)-COII region. The sequences exhibited AT bias typical of insect mitochondrial DNA which was highest in the first codon position of COI and in the third codon position of COII. There was no phylogeographic distribution of the populations. The Fu and Li's D and F tests were non-significant for this mitochondrial region. No Wolbachia infection was detected in any of the populations. The genetic differentiation amongst the populations was highly significant (p < 0.001; chi(2) = 123.333; df = 75), suggesting restricted gene flow between the populations. This result did not correlate with that obtained with nuclear rDNA markers i.e. ITS1 and ITS2, suggesting a male biased gene flow between the populations.

10aBanana pseudostem weevil10aCOI-tRNA(Leu) -COII10aGenetic differentiation10aMale biased gene flow10aOdoiporus longicollis (Oliver)1 aShankar, Pallavi1 aKulkarni, Vishvas, M.1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/male-biased-gene-flow-banana-pseudostem-weevil-odoiporus-longicollis-oliver-revealed-002731nas a2200289 4500008004100000022001400041245010000055210006900155260007400224300001200298490000800310520170400318653001602022653001702038653002402055653003602079100002302115700002502138700002402163700002302187700001502210700001602225700002402241700002602265700002602291856012402317 2016 eng d a0003-607200aStreptomyces lonarensis sp nov., isolated from lonar lake, a meteorite salt water lake in India0 aStreptomyces lonarensis sp nov isolated from lonar lake a meteor aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a225-2350 v1093 a

A novel alkaliphilic actinomycete, strain NCL716(T), was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7-11 at 28 A degrees C. The cell wall was found to contain ll-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C-16:0 (46.8 %), C-17:1 (12.4 %), anteiso-C-15:0 (5.1 %) and anteiso-C-17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H-6) (70.3 %), MK-9 (H-4) (15.5 %) and MK-9 (H-8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716(T) shares > 99 % similarity with that of Streptomyces bohaiensis strain 11A07(T), DNA-DNA hybridization revealed only 33.2 +/- A 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716(T) (=DSM 42084(T) = MTCC 11708(T) = KCTC 39684(T)).

10aAlkaliphile10aDDH analysis10aPolyphasic taxonomy10aStreptomyces lonarensis sp nov.1 aSharma, Trupti, K.1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aShinde, Vidhya, K.1 aZhan, Jing1 aLi, Wen-Jun1 aRele, Meenakshi, V.1 aDastager, Syed, Gulam1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/streptomyces-lonarensis-sp-nov-isolated-lonar-lake-meteorite-salt-water-lake-india-002839nas a2200325 4500008004100000245014800041210006900189260000800258490000700266520174000273653001002013653002202023653001902045653002602064653001402090653002202104653002202126653002702148653001202175653001402187653000802201653001002209653004502219653002702264100002802291700001902319700001902338700002602357856013002383 2017 eng d00aGenetic variability in Indian populations of banana corm weevil [Cosmopolites sordidus (Coleoptera: Curculionidae)] assessed by RAPDs and AFLPs0 aGenetic variability in Indian populations of banana corm weevil  cSEP0 v373 a

Cosmopolites sordidus (Germar), commonly known as banana corm weevil, is an important economic pest in Asia that can cause severe yield loss depending upon the stage at which infestation occurs. In spite of its economic importance, little is known about the population structure of this pest in India. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) were used to characterize the population genetic structure of C. sordidus collected from five hot spot locations in India. Nineteen RAPD primers and five selective AFLP primer combinations generated 147 and 304 amplification products, respectively. UPGMA dendrograms generated with both marker systems failed to reveal populations clustered based on geographic distance, which was confirmed by the Mantel test, which did not show a strong correlation between genetic distance and geographic distance. Values of indices of genetic identity showed that the populations were closely related. Though the gene flow estimate (Nm) between the populations was 0.469, suggesting restricted gene flow, the populations are not genetically distinct. These observations suggest that the range expansion of this banana pest in India has taken place through transport of infested corms and plant material, resulting in genetically close populations that are geographically distinct. These results provide important information on the population structure of this pest in India, which will aid in designing suitable strategies for its control and management, especially with respect to insecticide resistance.

10aAflps10aArbitrary Primers10aBemisia-tabaci10aCosmopolites Sordidus10adiversity10aGenetic diversity10aGermar Coleoptera10ainsecticide resistance10aMarkers10aMusa Spp.10aPcr10aRapds10aRestricted Gene Flow Natural-populations10aScirpophaga-incertulas1 aYadav, Sweta, Kumari U.1 aSingh, Jyotsna1 aPadmanaban, B.1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/genetic-variability-indian-populations-banana-corm-weevil-cosmopolites-sordidus-coleoptera01801nas a2200169 4500008004100000022001400041245015800055210006900213260000800282300001300290490000700303520112700310100002601437700002101463700002601484856012101510 2018 eng d a1742-758400aAnalyses of the internal transcribed rDNA spacers (ITS1 and ITS2) of Indian weevils of Odoiporus longicollis (Olivier) reveal gene flow between locations0 aAnalyses of the internal transcribed rDNA spacers ITS1 and ITS2  cDEC a313-329 0 v383 aAn important step towards developing a successful integrated pest management (IPM) programme for the control of banana pseudostem weevil, Odoiporus longicollis (Olivier), a serious pest of banana in India, is the study of the population structure of the pest. In the present study, the genetic variation among 30 individual weevils of O. longicollis collected from six different locations was assessed by analysing the primary nucleotide sequences of the rDNA ITS1 and ITS2 regions. AMOVA, Mantel test, and the maximum likelihood trees of the haplotypes failed to reveal any phylogeographic structuring, which was confirmed by haplotype analysis and genetic differentiation estimates. The results indicate that the locations are not differentiated, i.e. there is gene flow between the locations. The star-shaped networks revealed a signature of demographic expansion that was confirmed by the different demographic tests. These results provide important information, which is essential for the development of suitable strategies for the control of this banana pest, as well as management of its resistance to insecticides.1 aKumar, Lalitha, Sunil1 aShankar, Pallavi1 aKulkarni, Vishvas, M. uhttps://library.ncl.res.in/content/analyses-internal-transcribed-rdna-spacers-its1-and-its2-indian-weevils-odoiporus01814nas a2200217 4500008004100000022001400041245018100055210006900236260000800305300001000313490000700323520097900330653003501309653001801344653000901362653000901371653003601380653002401416100002601440856013001466 2019 eng d a1742-758400aSecondary structure of the internal transcribed rDNA (ITS) regions of cosmopolites sordidus (Germar) and odoiporus longicollis (Olivier): a first report in family Curculionidae0 aSecondary structure of the internal transcribed rDNA ITS regions cMAR a53-610 v393 a

In the present work the consensus secondary structure of the internal transcribed rDNA spacers ITS1 and ITS2 of two weevils i.e. Cosmopolites sordidus (Germar) and Odoiporus longicollis (Olivier) has been described. The secondary structures of the ITS1 region of C.sordidus and O.longicollis have ten and five helices respectively, emerging from a central core. Two and five helices emerge from a central core in the ITS2 secondary structures of C.sordidus and O.longicollis respectively, and do not conform to the `paneukaryotic' four domain model. The ITS2 sequences of these two weevils share three regions with maximum sequence identity. Of these, two are present at the 5' and 3' end and share structural similarity, suggesting their possible role in the processing of the rRNA. This is the first report of the secondary structure of the ITS region in family Curculionidae which can serve as a valuable resource for homology modelling and phylogenetic studies.

10aCosmopolites sordidus (Germar)10aCurculionidae10aITS110aITS210aOdoiporus longicollis (Olivier)10aSecondary structure1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/secondary-structure-internal-transcribed-rdna-its-regions-cosmopolites-sordidus-germar-and