02126nas a2200217   4500008004100000022001400041245009900055210006900154260007100223300001400294490000700308520131800315100001801633700002201651700001601673700002601689700002101715700002001736700002201756856013001778       2012                        eng d  a1525-779700aControlled synthesis of O-glycopolypeptide polymers and their molecular recognition by lectins0 aControlled synthesis of Oglycopolypeptide polymers and their mol  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAY  a1287-12950 v133 a<p>The facile synthesis of high molecular weight water-soluble O-glycopolypeptide polymers by the ring-opening polymerization of their corresponding N-carboxyanhydride (NCA) in very high yield (overall yield &gt; 70%) is reported. The per-acetylated-O-glycosylated lysine-NCA monomers, synthesized using stable glycosyl donors and a commercially available protected amino acid in very high yield, was polymerized using commercially available amine initiators. The synthesized water-soluble glycopolypeptides were found to be alpha-helical in aqueous solution. However, we were able to control the secondary conformation of the glycopolypeptides (alpha-helix vs nonhelical structures) by polymerizing raceznic amino acid glyco NCAs. We have also investigated the binding of the glycopolypeptide poly(alpha-manno-O-lys) with the lectin Con-A using precipitation and hemagglutination assays as well as by isothermal titration calorimetry (ITC). The ITC results clearly show that the binding process is enthalpy driven for both alpha-helical and nonhelical structures, with negative entropic contribution. Binding stoichiometry for the glycopolypeptide poly(alpha-manno-O-lys) having a nonhelical structure was slightly higher as compared to the corresponding polypeptide which adopted an alpha-helical structure.</p>1 aPati, Debasis1 aShaikh, Ashif, Y.1 aDas, Soumen1 aNareddy, Pavan, Kumar1 aSwamy, Musti, J.1 aHotha, Srinivas1 aGupta, Sayam, Sen  uhttps://library.ncl.res.in/content/controlled-synthesis-o-glycopolypeptide-polymers-and-their-molecular-recognition-lectins-002237nas a2200205   4500008004100000022001400041245012400055210006900179260007100248300001400319490000700333520144600340100002201786700001601808700001801824700002001842700002201862700002001884856012701904       2014                        eng d  a1525-779700aCationic charged helical glycopolypeptide using ring opening polymerization of 6-deoxy-6-azido-glyco-N-carboxyanhydride0 aCationic charged helical glycopolypeptide using ring opening pol  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcOCT  a3679-36860 v153 a<p>Glycopolypeptides with a defined secondary structure are of significance in understanding biological phenomena. Synthetic glycopolypeptides, or polypeptides featuring pendant carbohydrate moieties, have been of particular interest in the field of tissue engineering and drug delivery. In this work, we have synthesized charged water-soluble glycopolypeptides that adopt a helical conformation in water. This was carried out by the synthesis of a glyco-N-carboxyanhydride (glyco-NCA) containing an azide group at the sixth position of the carbohydrate ring. Subsequently, the NCA was polymerized to obtain azide-containing glycopolypeptides having good control over molecular weight and polydispersity index (PDI) in high yields. We were also able to control the incorporation of the azide group by synthesizing random co-glycopolypeptide containing 6-deoxy-6-azido and regular 6-OAc functionalized glucose. This azide functionality allows for the easy attachment of a bioactive group, which could potentially enhance the biological activity of the glycopolypeptide. We were able to obtain water-soluble charged glycopolypeptides by both reducing the azide groups into amines and using CuAAC with propargylamine. These charged glycopolypeptides were shown to have a helical conformation in water. Preliminary studies showed that these charged glycopolypeptides showed good biocompatibility and were efficiently taken up by HepG2 cells.</p>1 aShaikh, Ashif, Y.1 aDas, Soumen1 aPati, Debasis1 aDhaware, Vinita1 aGupta, Sayam, Sen1 aHotha, Srinivas  uhttps://library.ncl.res.in/content/cationic-charged-helical-glycopolypeptide-using-ring-opening-polymerization-6-deoxy-6-0