02668nas a2200277   4500008004100000022001400041245008000055210006900135260007400204300001200278490000800290520174300298653001502041653002402056653003302080653001302113100002402126700002502150700002602175700001402201700002002215700001702235700001602252700001602268856010602284       2014                        eng d  a0003-607200aRoseovarius azorensis sp nov., isolated from seawater at espalamaca, azores0 aRoseovarius azorensis sp nov isolated from seawater at espalamac  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR  a571-5780 v1053 a<p>A Gram-negative, motile, non-spore forming, rod shaped aerobic bacterium, designated strain SSW084(T), was isolated from a surface seawater sample collected at Espalamaca (38A degrees 33'N; 28A degrees 39'W), Azores. Growth was found to occur from 15 to 40 A degrees C (optimum 30 A degrees C), at pH 7.0-9.0 (optimum pH 7.0) and with 25-100 % seawater or 0.5-7.0 % NaCl in the presence of Mg2+ and Ca2+; no growth was found with NaCl alone. Colonies on seawater nutrient agar were observed to be punctiform, white, convex, circular, smooth, and translucent. Strain SSW084(T) did not grow on Zobell marine agar and tryptic soy agar even when seawater supplemented. The major respiratory quinone was found to be Q-10 and the G + C content was determined to be 61.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SSW084(T) belongs to the genus Roseovarius and that its closest neighbours are Roseovarius tolerans EL-172(T), Roseovarius mucosus DFL-24(T) and Roseovarius lutimaris 112(T) with 95.7, 95.4 and 95.3 % sequence similarity respectively. The remaining species of Roseovarius showed &lt; 95 % similarity. The polar lipids of strain SSW084(T) were determined to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified lipid and one unidentified aminolipid. The major fatty acids identified were identified as C-18:1 omega 7c (52.5 %) and C-16:0 (13.8 %). On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain SSW084(T) is considered to represent a novel species of the genus Roseovarius, for which Roseovarius azorensis sp. nov is proposed. The type strain is SSW084(T) (=KCTC 32421(T) = MTCC 11812(T)).</p>10aEspalamaca10aPolyphasic taxonomy10aRoseovarius azorensis sp nov10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana  uhttps://library.ncl.res.in/content/roseovarius-azorensis-sp-nov-isolated-seawater-espalamaca-azores-002522nas a2200301   4500008004100000022001400041245012300055210006900178260000800247300001200255490000800267520152700275653001501802653002801817653002801845653002401873653001301897100002401910700002501934700002001959700002601979700001402005700002002019700001702039700001602056700001602072856013202088       2015                        eng d  a0003-607200aNioella nitratireducens gen. nov., sp nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island0 aNioella nitratireducens gen nov sp nov a novel member of the fam  cFEB  a589-5950 v1073 a<p>A novel Gram-negative, non-spore forming, rod-shaped aerobic bacterium, designated SSW136(T), was isolated from a surface seawater sample collected at Espalamaca (in Faial Island), Azores. Growth was found to occur from 10 to 37 A degrees C, pH 6.0-8.0, and with 2-11 % of NaCl. 16S rRNA gene sequence indicated that the strain SSW136(T) belongs to the family Rhodobacteraceae. Strain SSW136(T) exhibited 96.3, 95.9, 95.7 and 95.5 sequence similarity to the type strains Oceanicola litoreus M-M22(T), Roseovarius aestuarii SMK-122(T), Marivita geojedonensis DPG-138(T), and Pseudoruegeria aquimaris SW-255(T) respectively. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSW136(T) was affiliated to the family Rhodobacteraceae and formed a separate branch. The G+C content was 63.5 mol%. The major respiratory quinone was found to be Q-10. The polar lipids of strain SSW136(T) consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and three unidentified phospholipids. The major fatty acids were C-18:1 omega 7c (46.5 %), Cyclo-C-19:0 omega 8c (16.0 %) and C-16:0 (12.8 %). On the basis of the morphological, genotypic, chemotaxonomic characteristics and low DNA-DNA relatedness, strain SSW136(T) is proposed to represent a novel genus and novel species, Nioella nitratireducens gen. nov., sp. nov., in the family Rhodobacteraceae. The type strain is SSW136(T) (=KCTC 32417(T) = NCIM 5499(T)).</p>10aEspalamaca10aNioella nitratireducens10aNovel genus and species10aPolyphasic taxonomy10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aYoon, Jung-Hoon1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana  uhttps://library.ncl.res.in/content/nioella-nitratireducens-gen-nov-sp-nov-novel-member-family-rhodobacteraceae-isolated-azorean