02668nas a2200277 4500008004100000022001400041245008000055210006900135260007400204300001200278490000800290520174300298653001502041653002402056653003302080653001302113100002402126700002502150700002602175700001402201700002002215700001702235700001602252700001602268856010602284 2014 eng d a0003-607200aRoseovarius azorensis sp nov., isolated from seawater at espalamaca, azores0 aRoseovarius azorensis sp nov isolated from seawater at espalamac aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR a571-5780 v1053 a

A Gram-negative, motile, non-spore forming, rod shaped aerobic bacterium, designated strain SSW084(T), was isolated from a surface seawater sample collected at Espalamaca (38A degrees 33'N; 28A degrees 39'W), Azores. Growth was found to occur from 15 to 40 A degrees C (optimum 30 A degrees C), at pH 7.0-9.0 (optimum pH 7.0) and with 25-100 % seawater or 0.5-7.0 % NaCl in the presence of Mg2+ and Ca2+; no growth was found with NaCl alone. Colonies on seawater nutrient agar were observed to be punctiform, white, convex, circular, smooth, and translucent. Strain SSW084(T) did not grow on Zobell marine agar and tryptic soy agar even when seawater supplemented. The major respiratory quinone was found to be Q-10 and the G + C content was determined to be 61.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SSW084(T) belongs to the genus Roseovarius and that its closest neighbours are Roseovarius tolerans EL-172(T), Roseovarius mucosus DFL-24(T) and Roseovarius lutimaris 112(T) with 95.7, 95.4 and 95.3 % sequence similarity respectively. The remaining species of Roseovarius showed < 95 % similarity. The polar lipids of strain SSW084(T) were determined to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified lipid and one unidentified aminolipid. The major fatty acids identified were identified as C-18:1 omega 7c (52.5 %) and C-16:0 (13.8 %). On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain SSW084(T) is considered to represent a novel species of the genus Roseovarius, for which Roseovarius azorensis sp. nov is proposed. The type strain is SSW084(T) (=KCTC 32421(T) = MTCC 11812(T)).

10aEspalamaca10aPolyphasic taxonomy10aRoseovarius azorensis sp nov10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana uhttps://library.ncl.res.in/content/roseovarius-azorensis-sp-nov-isolated-seawater-espalamaca-azores-002579nas a2200265 4500008004100000022001400041245014800055210006900203260007400272300001400346490000800360520159000368653001101958653003701969653002202006653002402028653001302052100002402065700002502089700002602114700001402140700001602154700001602170856012702186 2015 eng d a0003-607200aCitreicella manganoxidans sp nov., a novel manganese oxidizing bacterium isolated from a shallow water hydrothermal vent in Espalamaca (Azores)0 aCitreicella manganoxidans sp nov a novel manganese oxidizing bac aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC a1433-14390 v1083 a

A Gram-stain negative, non-motile, non-spore forming, aerobic and rod or narrow lemon-shaped bacterial strain, VSW210(T), was isolated from surface seawater in a shallow water hydrothermal vent region in Espalamaca (Azores). Strain VSW210(T) was found to grow optimally at 30 A degrees C, at pH 7 and in the presence of 2-6 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain VSW210(T) clusters with the type strain Citreicella marina CK-I3-6(T) (sequence similarity value of 99.6 %), but DNA-DNA hybridization showed DNA-DNA relatedness between the strain VSW210(T) and C. marina CK-I3-6(T) to be 55.8 +/- A 3.2 %. The DNA G+C content of strain VSW210(T) was determined to be 67.4 mol%. The cellular fatty acid profiles of strain VSW210(T) was found to contain C-18:1 omega 7c (80.1 %) and C-16:0 (9.2 %). The major polar lipids in strain VSW210(T) were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid. Strain VSW210(T) was found to be able to oxidize soluble Mn(II) to insoluble MnO2, which was confirmed with LBB staining. Differential phenotypic properties and genetic uniqueness revealed that this strain VSW210(T) is distinguishable from other species of the genus Citreicella. On the basis of the data presented, strain VSW210(T) is considered to represent a novel species of the genus Citreicella, for which the name Citreicella manganoxidans sp. nov. is proposed. The type strain is VSW210(T) (=KCTC 32497(T) = MCC 2286(T)).

10aAzores10aCitreicella manganoxidans sp nov10aHydrothermal vent10aPolyphasic taxonomy10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aDastager, Syed, Gulam1 aLiu, Qing1 aLi, Wen-Jun1 aColaco, Ana uhttps://library.ncl.res.in/content/citreicella-manganoxidans-sp-nov-novel-manganese-oxidizing-bacterium-isolated-shallow-002522nas a2200301 4500008004100000022001400041245012300055210006900178260000800247300001200255490000800267520152700275653001501802653002801817653002801845653002401873653001301897100002401910700002501934700002001959700002601979700001402005700002002019700001702039700001602056700001602072856013202088 2015 eng d a0003-607200aNioella nitratireducens gen. nov., sp nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island0 aNioella nitratireducens gen nov sp nov a novel member of the fam cFEB a589-5950 v1073 a

A novel Gram-negative, non-spore forming, rod-shaped aerobic bacterium, designated SSW136(T), was isolated from a surface seawater sample collected at Espalamaca (in Faial Island), Azores. Growth was found to occur from 10 to 37 A degrees C, pH 6.0-8.0, and with 2-11 % of NaCl. 16S rRNA gene sequence indicated that the strain SSW136(T) belongs to the family Rhodobacteraceae. Strain SSW136(T) exhibited 96.3, 95.9, 95.7 and 95.5 sequence similarity to the type strains Oceanicola litoreus M-M22(T), Roseovarius aestuarii SMK-122(T), Marivita geojedonensis DPG-138(T), and Pseudoruegeria aquimaris SW-255(T) respectively. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSW136(T) was affiliated to the family Rhodobacteraceae and formed a separate branch. The G+C content was 63.5 mol%. The major respiratory quinone was found to be Q-10. The polar lipids of strain SSW136(T) consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and three unidentified phospholipids. The major fatty acids were C-18:1 omega 7c (46.5 %), Cyclo-C-19:0 omega 8c (16.0 %) and C-16:0 (12.8 %). On the basis of the morphological, genotypic, chemotaxonomic characteristics and low DNA-DNA relatedness, strain SSW136(T) is proposed to represent a novel genus and novel species, Nioella nitratireducens gen. nov., sp. nov., in the family Rhodobacteraceae. The type strain is SSW136(T) (=KCTC 32417(T) = NCIM 5499(T)).

10aEspalamaca10aNioella nitratireducens10aNovel genus and species10aPolyphasic taxonomy10aSeawater1 aRajasabapathy, Raju1 aMohandass, Chellandi1 aYoon, Jung-Hoon1 aDastager, Syed, Gulam1 aLiu, Qing1 aKhieu, Thi-Nhan1 aSon, Chu, Ky1 aLi, Wen-Jun1 aColaco, Ana uhttps://library.ncl.res.in/content/nioella-nitratireducens-gen-nov-sp-nov-novel-member-family-rhodobacteraceae-isolated-azorean02731nas a2200289 4500008004100000022001400041245010000055210006900155260007400224300001200298490000800310520170400318653001602022653001702038653002402055653003602079100002302115700002502138700002402163700002302187700001502210700001602225700002402241700002602265700002602291856012402317 2016 eng d a0003-607200aStreptomyces lonarensis sp nov., isolated from lonar lake, a meteorite salt water lake in India0 aStreptomyces lonarensis sp nov isolated from lonar lake a meteor aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a225-2350 v1093 a

A novel alkaliphilic actinomycete, strain NCL716(T), was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7-11 at 28 A degrees C. The cell wall was found to contain ll-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C-16:0 (46.8 %), C-17:1 (12.4 %), anteiso-C-15:0 (5.1 %) and anteiso-C-17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H-6) (70.3 %), MK-9 (H-4) (15.5 %) and MK-9 (H-8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716(T) shares > 99 % similarity with that of Streptomyces bohaiensis strain 11A07(T), DNA-DNA hybridization revealed only 33.2 +/- A 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716(T) (=DSM 42084(T) = MTCC 11708(T) = KCTC 39684(T)).

10aAlkaliphile10aDDH analysis10aPolyphasic taxonomy10aStreptomyces lonarensis sp nov.1 aSharma, Trupti, K.1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aShinde, Vidhya, K.1 aZhan, Jing1 aLi, Wen-Jun1 aRele, Meenakshi, V.1 aDastager, Syed, Gulam1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/streptomyces-lonarensis-sp-nov-isolated-lonar-lake-meteorite-salt-water-lake-india-0