01490nas a2200253   4500008004100000022001400041245008400055210006900139260010600208300001400314490000700328520053700335653002800872653002100900653002200921653002300943653002000966100002100986700002301007700002901030700002801059700003701087856011201124       2014                        eng d  a0040-403900aFungi mediated production and practical purification of (R)-(-)-3-quinuclidinol0 aFungi mediated production and practical purification of R3quinuc  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcOCT  a5911-59140 v553 a<p>A fungal system belonging to Mucoraceae family (Mucor piriformis) was explored for the asymmetric reduction of a prochiral ketone, 3-quinuclidinone (I) in an efficient manner to produce an important pharmaceutical precursor (R)-(-)-3-quinuclidinol (II) with similar to 96% enantiomeric excess. The efficiency of the process was improved by developing a cation exchange resin (Amberlite IR-120) which assisted the purification of water soluble metabolite II from fermentation media. (C) 2014 Elsevier Ltd. All rights reserved.</p>10a(R)-(-)-3-Quinuclidinol10aAmberlite IR-12010aBiotransformation10aIon exchange resin10aMucor pinformis1 aKolet, Swati, P.1 aJadhav, Dipesh, D.1 aPriyadarshini, Balaraman1 aSwarge, Bhagyashree, N.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/fungi-mediated-production-and-practical-purification-r-3-quinuclidinol-001705nas a2200241   4500008004100000022001400041245008800055210006900143260010600212300001400318490000700332520081800339653001101157653001901168653002301187653001101210653002201221100001901243700002101262700002301283700003701306856012001343       2014                        eng d  a0040-403900aLipase mediated separation of triterpene structural isomers, alpha- and beta-amyrin0 aLipase mediated separation of triterpene structural isomers alph  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcMAY  a3122-31250 v553 a<p>Pentacyclic triterpenoids alpha- and beta-amyrin possess a wide range of biological and pharmacological activities. High structural similarity between these two structural isomers makes their chromatographic separation an ineffective and tedious choice. In this study, Candida rugosa lipase catalyzed separation protocol for the isolation of individual isomers has been developed. In the presence of vinyl acetate as the acyl donor, Candida rugosa lipase carried out acetylation of beta-amyrin more efficiently as compared to alpha-amyrin leading to a kinetic separation. The conditions of transesterification reaction were optimized systematically, which was utilized to separate alpha- and beta-amyrin from a mixture obtained from the latex of Plumeria obtusa. (C) 2014 Elsevier Ltd. All rights reserved.</p>10aAmyrin10aCandida rugosa10aKinetic separation10aLipase10aStructural isomer1 aHaldar, Saikat1 aKale, Balaji, S.1 aJadhav, Dipesh, D.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/lipase-mediated-separation-triterpene-structural-isomers-alpha-and-beta-amyrin-001187nas a2200169   4500008004100000245010300041210006900144260000800213300001400221490000700235520054100242100002300783700002300806700002100829700003700850856013000887       2016                        eng d00aFungal mediated kinetic resolution of racemic acetates to (R)-alcohols using fusarium proliferatum0 aFungal mediated kinetic resolution of racemic acetates to Ralcoh  cOCT  a4563-45670 v573 a<p>Fungal mediated kinetic resolution of seven acyclic/aromatic acetates was achieved using Fusarium proliferatum to furnish (R)-alcohols in high enantiomeric excess (&gt;95%). The kinetic resolution was established as one-pot two-step de-esterification/oxidation biocatalytic process. Further, the preparative scale synthesis of (R)-(+)-1-phenylethanol was accomplished through de-esterification/oxidation of (+/-)-1-phenylethyl acetate using the whole cell of F. proliferatum NCIM 1105. (C) 2016 Elsevier Ltd. All rights reserved.</p>1 aJadhav, Dipesh, D.1 aPatil, Harshal, S.1 aChaya, Patil, S.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/fungal-mediated-kinetic-resolution-racemic-acetates-r-alcohols-using-fusarium-proliferatum03063nas a2200361   4500008004100000245013600041210006900177260000800246490000700254520182400261653003802085653001502123653002002138653002202158653001802180653003602198653001602234653002602250653003202276653002102308653002102329653001702350653002002367653002802387653001902415100001902434700001902453700002302472700003702495700002102532700001902553856012902572       2017                        eng d00aOral feeding with polyunsaturated fatty acids fosters hematopoiesis and thrombopoiesis in healthy and bone marrow-transplanted mice0 aOral feeding with polyunsaturated fatty acids fosters hematopoie  csep0 v473 a<p><span style="color: rgb(51, 51, 51); font-family: arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(248, 248, 248);">Hematopoietic stem cells play the vital role of maintaining appropriate levels of cells in blood. Therefore, regulation of their fate is essential for their effective therapeutic use. Here we report the role of polyunsaturated fatty acids (PUFAs) in regulating hematopoiesis which has not been explored well so far. Mice were fed daily for 10 days with n-6/n-3 PUFAs, viz. linoleic acid (LA), arachidonic acid (AA), alpha-linolenic acid and docosahexanoic acid (DHA) in four separate test groups with phosphate-buffered saline fed mice as control set. The bone marrow cells of PUFA-fed mice showed a significantly higher hematopoiesis as assessed using side population, Lin-Sca-1(+)ckit+, colony-forming unit (CFU), long-term culture, CFU-spleen assay and engraftment potential as compared to the control set. Thrombopoiesis was also stimulated in PUFA-fed mice. A combination of DHA and AA was found to be more effective than when either was fed individually. Higher incorporation of PUFAs as well as products of their metabolism was observed in the bone marrow cells of PUFA-fed mice. A stimulation of the Wnt, CXCR4 and Notch1 pathways was observed in PUFA-fed mice. The clinical relevance of this study was evident when bone marrow-transplanted recipient mice, which were fed with PUFAs, showed higher engraftment of donor cells, suggesting that the bone marrow microenvironment may also be stimulated by feeding with PUFAs. These data indicate that oral administration of PUFAs in mice stimulates hematopoiesis and thrombopoiesis and could serve as a valuable supplemental therapy in situations of hematopoietic failure. (C) 2017 Elsevier Inc. All rights reserved.</span></p>10aBone Marrow Transplantation (Bmt)10aCord Blood10aDown-regulation10aEx-vivo Expansion10aHematopoiesis10aHematopoietic Stem Cells (Hscs)10aLipid rafts10aMyocardial-infarction10aPolyunsaturated Fatty Acids10aProgenitor Cells10aProstaglandin-e210aSelf-renewal10aSpleen Colonies10aStem-cell Proliferation10aThrombopoiesis1 aLimbkar, Kedar1 aDhenge, Ankita1 aJadhav, Dipesh, D.1 aThulasiram, Hirekodathakallu, V.1 aKale, Vaijayanti1 aLimaye, Lalita  uhttps://library.ncl.res.in/content/oral-feeding-polyunsaturated-fatty-acids-fosters-hematopoiesis-and-thrombopoiesis-healthy01927nas a2200289   4500008004100000022001400041245009800055210006900153260000900222300001400231490000700245520098200252653001401234653002001248653002701268653001701295653001401312653001101326653002001337100002301357700002301380700001501403700002201418700003001440700003701470856013001507       2018                        eng d  a0960-894X00aRegioselective and efficient enzymatic synthesis of antimicrobial andrographolide derivatives0 aRegioselective and efficient enzymatic synthesis of antimicrobia  cAPR   a1132-11370 v283 a<p>Labdane diterpene andrographolide (1) is a major constituent of Andrographis paniculata and known to exhibit wide spectrum of biological activities. In this study, regioselective monoesters of (1) have been synthesized by using Amano lipase AK (Pseudomonas fluorescens) as a biocatalyst. Amano lipase AK was able to execute highly efficient esterification of hydroxyl group attached to C-14 carbon of (1) in presence of acyl donors. Among the various synthesized derivatives including two novel compounds such as andrographolide-14-propionate (3) and andrographolide-14-caproate (5) displayed antimicrobial activity against Staphylococcus aureus with low minimal inhibitory concentration (MIC) 4 mu g/mL and 16 mu g/mL respectively. Furthermore, they have shown low hemolysis activity at their respective MIC and increase in the permeability of the bacterial cell membrane as delineated by FITC uptake and SEM imaging studies. (C) 2018 Elsevier Ltd. All rights reserved.</p>10aAcylation10aAndrographolide10aAntimicrobial activity10abiocatalysis10aHemolysis10aLipase10aNatural product1 aPatil, Harshal, S.1 aJadhav, Dipesh, D.1 aPaul, Ajay1 aMulani, Fayaj, A.1 aKaregaonkar, Shrikant, J.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/regioselective-and-efficient-enzymatic-synthesis-antimicrobial-andrographolide-derivatives