02325nas a2200193   4500008004100000022001400041245006000055210005800115260011800173300001200291490000700303520162400310100002601934700001901960700003001979700001802009700001602027856008802043       2014                        eng d  a1466-502600aKocuria indica sp nov., isolated from a sediment sample0 aKocuria indica sp nov isolated from a sediment sample  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcMAR  a869-8740 v643 a<p>An actinobacterial strain was isolated from a sediment sample from Chorao Island, in Goa province, India, and subjected to a taxonomic investigation. The isolate, designated NIO-1021(T), was a Gram-stain positive, aerobic, non-motile and coccoid. Strain NIO-1021(T) was identified as a member of the genus Kocuria by a polyphasic approach. Strain NIO-1021(T) could be differentiated from other members of the genus Kocuria on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain NIO-1021(T) to the most closely related species, Kocuria marina KCTC 9943(T), was 98.6% with 19 nt differences). Furthermore, DNA-DNA hybridization analysis revealed that the novel strain had lower relatedness with the type strains of other members of the genus Kocuria. The strain formed a monophyletic clade with K. marina with 100% bootstrap values. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified lipids. The predominant menaquinone was MK-7(H-2). The major fatty acids were anteiso-C-15:0, iso-C-(16:0) and anteiso-C-17:0. The DNA G+C content of strain NIO-1021(T) was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification as representing a member of the genus Kocuria. On the basis of phenotypic, chemotypic and molecular characteristics, strain NIO-1021(T) is considered to represent a novel species of the genus Kocuria, for which the name Kocuria indica sp. nov. is proposed, with strain NIO-1021(T) (=NCIM 5455(T)=DSM 25126(T)=CCTCC AA 209050(T)) as the type strain.</p>1 aDastager, Syed, Gulam1 aTang, Shan-Kun1 aSrinivasan, Krishnamurthi1 aLee, Jae-Chan1 aLi, Wen-Jun  uhttps://library.ncl.res.in/content/kocuria-indica-sp-nov-isolated-sediment-sample-000625nas a2200181   4500008004100000022001400041245008400055210006900139260000800208300000900216490000700225100002300232700001900255700003000274700001800304700001600322856010500338       2017                        eng d  a1466-502600aKocuria indica sp. nov., isolated from a sediment sample (vol 64, pg 869, 2014)0 aKocuria indica sp nov isolated from a sediment sample vol 64 pg   cMAY  a16200 v671 aDastager, Syed, G.1 aTang, Shan-Kun1 aSrinivasan, Krishnamurthi1 aLee, Jae-Chan1 aLi, Wen-Jun  uhttps://library.ncl.res.in/content/kocuria-indica-sp-nov-isolated-sediment-sample-vol-64-pg-869-2014