02381nas a2200265   4500008004100000022001400041245014900055210006900204260009900273300001000372490000700382520140800389653000601797653000801803653003301811653002301844653001201867653001501879653001401894653002501908100001901933700001801952700001401970856013101984       2013                        eng d  a0964-830500aApproach towards biocatalytic valorisation of barley beta-glucan for bioethanol production using 1,3-1,4 beta-glucanase and thermotolerant yeast0 aApproach towards biocatalytic valorisation of barley betaglucan   aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcAUG  a81-860 v823 a<p>The exploitation of renewable resource containing polymers other than cellulose and hemicellulose are critically important for the feasibility of biofuel production. The potential of 1,3-1,4 glucan 4-gluconohydrolyase mediated saccharification of barley beta-glucan (BG) was investigated for ethanol production using thermotolerant Saccharomyces sp. A maximum hydrolysis of 71% was obtained in 24 h using in-house produced 1,3-1,4 beta-glucanase from an alkalothermophilic Thermomonospora sp. whereas the hydrolysis was 100% with Accellerase (TM) 1000. The synergistic effect of beta-glucosidase and 1,3-1,4 beta-glucanase was demonstrated by the exogenous addition of beta-glucosidase to Thermomonospora 1,3-1,4 beta-glucanase which resulted in complete hydrolysis of BG. The hydrolysates of BG obtained using Accellerase or a cocktail of Thermomonospora 1,3-1,4 beta-glucanase and beta-glucosidase when fermented with free cells of Saccharomyces at 40 degrees C produced an ethanol yield of 0.44 g g(-1) and 0.46 g g(-1) respectively and when fermented with immobilized cells produced a yield of 0.49 g g(-1). The Ca-alginate immobilized yeast cells were reused nine times at 40 degrees C with 100% fermentation efficiency. The economics of barley-to-fuel ethanol program will ameliorate if in addition to barley starch, beta-glucan is also utilized. (c) 2013 Elsevier Ltd. All rights reserved.</p>10a110a3-110a4 Glucan 4-gluconohydrolyase10aBarley beta-glucan10aEthanol10aHydrolysis10aSynergism10aThermotolerant yeast1 aDivate, Rupesh1 aMenon, Vishnu1 aRao, Mala  uhttps://library.ncl.res.in/content/approach-towards-biocatalytic-valorisation-barley-beta-glucan-bioethanol-production-using-0