02258nas a2200193   4500008004100000022001400041245011700055210006900172260011800241300001400359490000700373520143400380100002401814700002501838700003001863700001901893700002601912856012601938       2014                        eng d  a1466-502600aDomibacillus enclensis sp nov., isolated from marine sediment, and emended description of the genus domibacillus0 aDomibacillus enclensis sp nov isolated from marine sediment and   aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcDEC  a4098-41020 v643 a<p>A novel red-pigmented bacterial strain, designated NIO-1016(T), was isolated from a sediment sample from Chorao Island, India and was investigated by a polyphasic taxonomic approach. The strain was Gram-reaction-positive, strictly aerobic, motile and catalase-positive and produced spherical to slightly ellipsoidal endospores with subterminal position on swollen sporangia. The genomic DNA G+C content was 46.9 molok. The major fatty acids were anteiso-C-15:0, anteiso-C-17:0, iSO-C-15:0 and C-16:0. The predominant quinones were MK-6 (89.3 %) and MK-7 (8.7 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, and an unidentified phospholipid. meso-Diaminopimelic acid (type My) was present in the cell-wall peptidoglycan and the major whole-cell sugars were glucose and ribose. The closest phylogenetic neighbours were identified as Domibacillus robiginosus DSM 25058(T) (98.0% similarity) and Domibacillus indicus DSM 28032(T) (97.2% similarity), other species of the genus Bacillus displayed below 96% similarity. Phylogenetic, physiological, biochemical and morphological differences between strain NI0-1016(T) and its closest neighbours indicate that this strain represents a novel species in the genus Domibacillus in the family Bacillaceae for which the name Domibacillus enclensis sp. nov. is proposed with the type species N10-1016(T) (=DSM 25145(T)=NCIM 5462(T)=CCTCC AB 2011121(T)).</p>1 aSonalkar, Vidya, V.1 aMawlankar, Rahul, R.1 aKrishnamurthi, Srinivasan1 aTang, Shan-Kun1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/domibacillus-enclensis-sp-nov-isolated-marine-sediment-and-emended-description-genus-002460nas a2200253   4500008004100000022001400041245006500055210006300120260007400183300001200257490000800269520164200277653001301919653001401932653001501946653001301961100002401974700002501998700002002023700001802043700002402061700002602085856009502111       2015                        eng d  a0003-607200aBacillus filamentosus sp nov., isolated from sediment sample0 aBacillus filamentosus sp nov isolated from sediment sample  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB  a433-4410 v1073 a<p>A novel Gram-stain positive, endospore-forming bacterium, designated SGD-14(T), was isolated from a marine sediment sample in Goa Province, India. Cells of the isolate were found to be strictly aerobic. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SGD-14(T) showed a similarity of 99.5 % with Bacillus endophyticus and similarities to other Bacillus type strains were below 96 %. The whole-cell sugar pattern was found to consist of ribose, xylose and glucose. The predominant menaquinone was identified as MK-7 and the major fatty acids as anteiso-C-15:0, iso-C-15:0, iso-C-16:0, anteiso-C-17:0, C-16:0 and iso-C-14:0. The strain was found to grow optimally at 30 A degrees C and pH 7.0-7.5. DNA G + C content was determined to be 39.6 mol%. The phospholipid pattern was found to consist of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. DNA-DNA hybridization studies between strain SGD-14(T) and B. endophyticus CIP106778(T) showed that strain SGD-14(T) exhibited &lt; 70 % similarity with B. endophyticus CIP106778(T). Differences in phenotypic and chemotaxonomic characteristics between the novel strain and B. endophyticus CIP106778(T) further confirmed that this isolate represents novel species. Phylogenetic analysis showed that strain SGD-14(T) fits in the same clade with B. endophyticus with 100 % bootstrap values. Strain SGD-14(T) is therefore considered to represent a novel species of the genus Bacillus, for which the name Bacillus filamentosus sp. nov. is proposed. The type strain of Bacillus filamentosus is SGD-14(T) = (=NCIM 5491(T) = DSM 27955(T)).</p>10aBacillus10aFilaments10aPolyphasic10aSediment1 aSonalkar, Vidya, V.1 aMawlankar, Rahul, R.1 aRamana, Venkata1 aJoseph, Neeta1 aShouche, Yogesh, S.1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/bacillus-filamentosus-sp-nov-isolated-sediment-sample-002389nas a2200265   4500008004100000022001400041245007400055210006900129260007400198300001200272490000800284520150300292653001301795653001601808653002101824653001501845100002401860700002501884700002401909700002001933700001801953700002401971700002601995856010202021       2015                        eng d  a0003-607200aDeinococcus enclensis sp nov., isolated from a marine sediment sample0 aDeinococcus enclensis sp nov isolated from a marine sediment sam  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN  a141-1480 v1073 a<p>A novel pale-pink coloured strain, designated NIO-1023(T), was isolated from a marine sediment sample from Chorao Island, Goa, India. The taxonomic position of strain NIO-1023(T) was investigated by using a polyphasic approach. The cells were observed to be Gram-stain positive, coccal shaped and non-spore forming. Phylogenetic analyses using the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The strain NIO-1023(T) showed highest 16S rRNA gene sequence similarities with Deinococcus ficus (97.8 %), whereas other Deinococcus species showed less than 95 % sequence similarity. The DNA-DNA relatedness with respect to D. ficus CC-FR2-10(T) was 23.9 %. Chemotaxonomic data revealed that strain NIO-1023(T) contains only menaquinone MK-8 as the respiratory quinone and a complex polar lipid profile consisting of different unidentified glycolipids and polar lipids, two unknown phospholipids and three unknown phosphoglycolipids. As in other deinococci, one of these phosphoglycolipids was predominant in the profile. The predominant fatty acids were identified as C-17:1 w8c, C-16:1 w6c/w7c, C-15:1 w6c and C-17:1 w9c. The genomic DNA G + C content of strain NIO-1023(T) was determined to be 67.2 mol%. The biochemical and chemotaxonomic properties demonstrate that strain NIO-1023(T) represents a novel species, for which the name Deinococcus enclensis sp. nov. is proposed. The type strain is NIO-1023(T) (=DSM 25127(T) = NCIM 5456(T)).</p>10a16S rRNA10aDeinococcus10aMarine sediments10aPolyphasic1 aThorat, Meghana, N.1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aRamana, Venkata1 aJoseph, Neeta1 aShouche, Yogesh, S.1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/deinococcus-enclensis-sp-nov-isolated-marine-sediment-sample-002018nas a2200241   4500008004100000022001400041245006100055210005900116260011800175300001400293490000700307520117200314100002601486700002501512700002401537700002401561700001701585700001801602700003001620700001901650700001601669856009101685       2015                        eng d  a1466-502600aExiguobacterium enclense sp nov., isolated from sediment0 aExiguobacterium enclense sp nov isolated from sediment  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcMAY  a1611-16160 v653 a<p>A Gram-stain-positive bacterium, designated strain NIO-1109(T), was isolated from a marine sediment sample from Chorao Island, Goa, India. Phenotypic and chemotaxonomic characteristics and data from phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1109(T) was related to the genus Exiguobacterium. Strain NIO-1109(T) exhibited &gt;98.0% 16S rRNA gene sequence similarity with respect to Exiguobacterium indicum HHS 31(T) (99.5%) and Exiguobacterium acetylicum NCIMB 9889(T) (99.1 %); the type strains of other species showed &lt;98% similarity. Levels of DNA DNA relatedness between strain NIO-1109(T) and E. acetylicum DSM 20416(T) and E indicum LMG 23471(T) were less than 70% (33.0 +/- 2.0 and 37 +/- 32%, respectively). Strain NIO-1109(T) also differed from these two closely related species in a number of phenotypic traits. Based on phenotypic, chemotaxonomic and phylogenetic data, strain NIO-1109(T) is considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium enclense sp. nov. is proposed. The type strain is NIO-1109(T) (=NCIM 5457(T)= DSM 25128(T)=CCTCC AB 2011124(T)).</p>1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aThorat, Meghana, N.1 aMual, Poonam1 aVerma, Ashish1 aKrishnamurthi, Srinivasan1 aTang, Shan-Kun1 aLi, Wen-Jun  uhttps://library.ncl.res.in/content/exiguobacterium-enclense-sp-nov-isolated-sediment-002334nas a2200217   4500008004100000022001400041245006700055210006500122260011800187300001400305490000700319520152900326100002501855700001701880700002401897700002401921700001801945700003001963700002601993856009702019       2015                        eng d  a1466-502600aMicrobacterium enclense sp nov., isolated from sediment sample0 aMicrobacterium enclense sp nov isolated from sediment sample  aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcJUL  a2064-20700 v653 a<p>A novel bacterium (strain NIO-1002(T)) belonging to the genus Microbacterium was isolated from a marine sediment sample in Chorao Island, Goa Province, India. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Cells of this strain were Gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. It grew in 0-12 % (w/v) NaCl and at 25-37 degrees C, with optimal growth at 30 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1002(T) is associated with members of the genus Microbacterium, with highest sequence similarity with Microbacterium hominis CIP 105731(T) (98.1%) and Microbacterium testaceum KCTC 9103(T) (98.0 %). Within the phylogenetic tree, this novel strain shared a branching point with M. hominis CIP 105731(T). The DNA G+C content was 66.5 mol% and DNA DNA hybridization relatedness between NI-1002(T), M. hominis CIP 105731(T) and M. testaceum KCTC 9103(T) was 39.0 +/- 2.0 % and 41.0 +/- 2.0 %, respectively. The major fatty acids were ai-C-15:0, i-C-16:0 and al-C-17:0 and the diamino acid in the cell-wall peptidoglycan of NIO-1002(T) was lysine. Data obtained from DNA-DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain NIO-1002(T) represents a novel species within the genus Microbacterium. The name Microbacterium enclense sp. nov. is proposed, with NIO-1002(T) (=NCIM 5454(T)=DSM 25125(T)=CCTCC AB 2011120(T)) as the type strain.</p>1 aMawlankar, Rahul, R.1 aMual, Poonam1 aSonalkar, Vidya, V.1 aThorat, Meghana, N.1 aVerma, Ashish1 aSrinivasan, Krishnamurthi1 aDastager, Syed, Gulam  uhttps://library.ncl.res.in/content/microbacterium-enclense-sp-nov-isolated-sediment-sample-002159nas a2200229   4500008004100000022001400041245016500055210006900220260008600289300001200375490000700387520123900394653001001633653001801643653001601661653002501677100002901702700002401731700002301755700002101778856013001799       2016                        eng d  a1082-606800aPurification and partial characterization of novel penicillin V acylase from Acinetobacter sp AP24 isolated from loktak lake, an indo-burma biodiversity hotspot0 aPurification and partial characterization of novel penicillin V   a530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USAbTAYLOR & FRANCIS INCcOCT  a524-5300 v463 a<p>Members of the bacterial genus Acinetobacter have attracted great attention over the past few decades, on account of their various biotechnological applications and clinical implications. In this study, we are reporting the first experimental penicillin V acylase (PVA) activity from this genus. Penicillin acylases are pharmaceutically important enzymes widely used in the synthesis of semisynthetic beta-lactam antibiotics. The bacterium, identified as Acinetobacter sp. AP24, was isolated from the water of Loktak Lake (Manipur, India), an Indo-Burma biodiversity hotspot. PVA production was increased threefold in an optimized medium with 0.2% sodium glutamate and 1% glucose as nitrogen and carbon sources respectively, after 24hr of fermentation at 28 degrees C and pH 7.0 with shaking at 180rpm. The enzyme was purified to homogeneity by cation-exchange chromatography using SP-sepharose resin. The PVA is a homotetramer with subunit molecular mass of 34 kD. The enzyme was highly specific toward penicillin V with optimal hydrolytic activity at 40 degrees C and pH 7.5. The enzyme was stable from pH 5.0 to 9.0 at 25 degrees C for 2hr. The enzyme retained 75% activity after 1hr of incubation at 40 degrees C at pH 7.5.</p>10a6-APA10aAcinetobacter10aLoktak Lake10aPenicillin V acylase1 aPhilem, Pushparani, Devi1 aSonalkar, Vidya, V.1 aDharne, Mahesh, S.1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/purification-and-partial-characterization-novel-penicillin-v-acylase-acinetobacter-sp-ap2402731nas a2200289   4500008004100000022001400041245010000055210006900155260007400224300001200298490000800310520170400318653001602022653001702038653002402055653003602079100002302115700002502138700002402163700002302187700001502210700001602225700002402241700002602265700002602291856012402317       2016                        eng d  a0003-607200aStreptomyces lonarensis sp nov., isolated from lonar lake, a meteorite salt water lake in India0 aStreptomyces lonarensis sp nov isolated from lonar lake a meteor  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB  a225-2350 v1093 a<p>A novel alkaliphilic actinomycete, strain NCL716(T), was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7-11 at 28 A degrees C. The cell wall was found to contain ll-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C-16:0 (46.8 %), C-17:1 (12.4 %), anteiso-C-15:0 (5.1 %) and anteiso-C-17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H-6) (70.3 %), MK-9 (H-4) (15.5 %) and MK-9 (H-8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716(T) shares &gt; 99 % similarity with that of Streptomyces bohaiensis strain 11A07(T), DNA-DNA hybridization revealed only 33.2 +/- A 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716(T) (=DSM 42084(T) = MTCC 11708(T) = KCTC 39684(T)).</p>10aAlkaliphile10aDDH analysis10aPolyphasic taxonomy10aStreptomyces lonarensis sp nov.1 aSharma, Trupti, K.1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aShinde, Vidhya, K.1 aZhan, Jing1 aLi, Wen-Jun1 aRele, Meenakshi, V.1 aDastager, Syed, Gulam1 aKumar, Lalitha, Sunil  uhttps://library.ncl.res.in/content/streptomyces-lonarensis-sp-nov-isolated-lonar-lake-meteorite-salt-water-lake-india-000722nas a2200205   4500008004100000022001400041245009200055210006900147260000800216300000900224490000700233100002500240700001700265700002400282700002400306700001800330700003000348700002300378856011500401       2019                        eng d  a1466-502600aMicrobacterium enclense sp. nov., isolated from sediment sample (vol 65, pg 2064, 2015)0 aMicrobacterium enclense sp nov isolated from sediment sample vol  cMAY  a15280 v691 aMawlankar, Rahul, R.1 aMual, Poonam1 aSonalkar, Vidya, V.1 aThorat, Meghana, N.1 aVerma, Ashish1 aSrinivasan, Krishnamurthi1 aDastager, Syed, G.  uhttps://library.ncl.res.in/content/microbacterium-enclense-sp-nov-isolated-sediment-sample-vol-65-pg-2064-2015