02617nas a2200313   4500008004100000022001400041245015100055210006900206260000900275300000700284490000700291520154400298653001701842653001501859653002201874653001501896653001201911653000801923100001901931700003501950700003201985700002002017700002402037700002702061700002602088700002102114700003602135856013202171       2026                        eng d  a1029-842800aOral administration of different forms of exogenous tau protein induces multi-organ defects across developmental stages in drosophila melanogaster0 aOral administration of different forms of exogenous tau protein   cAUG   a430 v443 a<p>
	Tau is a crucial protein involved in many neurodegenerative diseases, including Alzheimer's disease. Natively, Tau is an unfolded protein found predominantly in axons that play a vital role in maintaining and stabilizing microtubules. Under pathological circumstances, Tau disengages from microtubules and forms insoluble intracellular filaments on its own, building neurofibrillary tangles in the brains of Alzheimer's patients. Although Tau exists in aggregates found in the brain and gut of Alzheimer's patients, the function of Tau in the brain is well studied, while the role of Tau in the gut needs further investigation. We have investigated the effects of Tau monomer, oligomer, and aggregates at 10 &amp; micro;M concentration via feeding them to the third instar larvae. To evaluate the genotoxicity, we did 4', 6-diamidino-2-phenylindole staining, while dichloro-dihydro-fluorescein diacetate staining was performed to assess cytotoxicity. Furthermore, we performed a TUNEL assay to detect apoptotic cells. Additionally, we carry out an NBT assay for biochemical estimation of reactive oxygen species by extracting hemolymph. Phenotypes were examined in different stages, i.e., larvae, pupae, and adult flies. Histological studies were performed on eye, gut tissue and reproductive organs of D. melanogaster to check the cytoskeletal alterations. The current study finds that the administration of monomer, oligomer, and aggregate forms causes genotoxicity, cytotoxicity, and cytoskeletal defects in D. melanogaster.</p>
10aCytoskeleton10aDrosophila10aneurodegeneration10aPhenotypes10aRhabdom10aTau1 aSahu, Surajita1 aChidamabaram, Hariharakrishnan1 aAnanthanarayanan, Vaishnavi1 aQureshi, Tazeen1 aDesale, SmitaEknath1 aChandrashekar, Madhura1 aSanthoshkumar, Rashmi1 aMishra, Monalisa1 aChinnathambi, Subashchandrabose  uhttps://library.ncl.res.in/content/oral-administration-different-forms-exogenous-tau-protein-induces-multi-organ-defects-across