02264nas a2200301   4500008004100000022001400041245011700055210006900172260009000241300002600331490000600357520118200363100002401545700002701569700001901596700002701615700002001642700002001662700001401682700001601696700002001712700002101732700002001753700002001773700001801793700001901811856013201830       2016                        eng d  a2041-172300aGAME9 regulates the biosynthesis of steroidal alkaloids and upstream isoprenoids in the plant mevalonate pathway0 aGAME9 regulates the biosynthesis of steroidal alkaloids and upst  aMACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLANDbNATURE PUBLISHING GROUPcFEB  aArticle Number: 106540 v73 a<p>Steroidal glycoalkaloids (SGAs) are cholesterol-derived molecules produced by solanaceous species. They contribute to pathogen defence but are toxic to humans and considered as anti-nutritional compounds. Here we show that GLYCOALKALOID METABOLISM 9 (GAME9), an APETALA2/Ethylene Response Factor, related to regulators of alkaloid production in tobacco and Catharanthus roseus, controls SGA biosynthesis. GAME9 knockdown and overexpression in tomato and potato alters expression of SGAs and upstream mevalonate pathway genes including the cholesterol biosynthesis gene STEROL SIDE CHAIN REDUCTASE 2 (SSR2). Levels of SGAs, C24-alkylsterols and the upstream mevalonate and cholesterol pathways intermediates are modified in these plants. Delta(7)-STEROL-C5(6)-DESATURASE (C5-SD) in the hitherto unresolved cholesterol pathway is a direct target of GAME9. Transactivation and promoter-binding assays show that GAME9 exerts its activity either directly or cooperatively with the SlMYC2 transcription factor as in the case of the C5-SD gene promoter. Our findings provide insight into the regulation of SGA biosynthesis and means for manipulating these metabolites in crops.</p>1 aCardenas, Pablo, D.1 aSonawane, Prashant, D.1 aPollier, Jacob1 aBossche, Robin, Vanden1 aDewangan, Veena1 aWeithorn, Efrat1 aTal, Lior1 aMeir, Sagit1 aRogachev, Ilana1 aMalitsky, Sergey1 aGiri, Ashok, P.1 aGoossens, Alain1 aBurdman, Saul1 aAharoni, Asaph  uhttps://library.ncl.res.in/content/game9-regulates-biosynthesis-steroidal-alkaloids-and-upstream-isoprenoids-plant-mevalonate-1