01287nas a2200157   4500008004100000022001400041245004300055210004100098260011000139300001600249490000600265520073700271100002001008700002601028856007501054       2013                        eng d  a2046-206900aBiomass to biodegradable polymer (PLA)0 aBiomass to biodegradable polymer PLA  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJUN  a13558-135680 v33 a<p>Lignocellulosic biomass is renewable and cheap, and it has the potential to displace fossil fuels for the production of fuels and chemicals. Biomass derived lactic acid is an important compound that can be used as a chemical platform for the production of a variety of important chemicals on a large scale. The quality of the monomers of lactic acid and lactide, as well as the chemical changes induced during polymerization and processing, are crucial parameters for controlling the properties of the resulting polylactic acid (PLA) products. In this review, we outline the process of exploiting biomass for the production of polylactic acid, a biodegradable polymer which is well-known as a sustainable bioplastic material.</p>1 aSinghvi, Mamata1 aGokhale, Digambar, V.  uhttps://library.ncl.res.in/content/biomass-biodegradable-polymer-pla-001828nas a2200217   4500008004100000022001400041245015700055210006900212260009900281300001200380490000800392520089100400653003201291653002601323653003501349653003201384100002001416700002101436700002601457856012701483       2013                        eng d  a0960-852400aSupplementation of medium with diammonium hydrogen phosphate enhanced the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity0 aSupplementation of medium with diammonium hydrogen phosphate enh  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcOCT  a736-7390 v1463 a<p>The production of D-lactic acid by Lactobacillus lactis RM2-24 was investigated using modified media to increase the efficiency of the fermentation process. The results indicated that the addition of 5 g/l peptone and 1 g/l (NH4)(2)HPO4 enhanced D-lactic acid production by 32%, as compared to that obtained from non supplemented media, with a productivity of 3.0 g/l/h. Lactate dehydrogenase (LDH) expression profile in these different media was studied which resulted in appearance of additional LDH isoform produced by cells when they were grown in HSYE supplemented with (NH4)(2)HPO4. The additional LDH appears to be L-LDH contributing to production of L-lactic acid in the fermented broth. This is totally new information in the lactic acid fermentation and could be very useful to industries engaged in D-lactic acid production. (c) 2013 Elsevier Ltd. All rights reserved.</p>10aD(-) lactic acid production10aLactate dehydrogenase10aLactobacillus delbrueckii Uc-310aLactobacillus lactis RM2-241 aSinghvi, Mamata1 aJadhav, Akanksha1 aGokhale, Digambar, V.  uhttps://library.ncl.res.in/content/supplementation-medium-diammonium-hydrogen-phosphate-enhanced-d-lactate-dehydrogenase-001981nas a2200181   4500008004100000022001400041245010600055210006900161260011000230300001400340490000600354520122700360100002001587700002001607700001701627700002601644856012901670       2015                        eng d  a2046-206900aBiocatalyst development for lactic acid production at acidic pH using inter-generic protoplast fusion0 aBiocatalyst development for lactic acid production at acidic pH   aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcDEC  a2024-20310 v53 a<p>Global warming and environmental problems force us to develop sustainable processes based on the use of biocatalysts that are eco-friendly with the least potential toxicity. Lactic acid fermentation at neutral pH generates a large amount of gypsum during down-stream processing. Hence it is essential to develop Lactobacillus strains which produce lactic acid at acidic pH thus making the whole downstream process environmentally friendly. Fusant F3 was generated using protoplast fusion between Lactobacillus delbrueckii Mut Uc-3 and Acetobacter pasteurianus NCIM 2314 on solid media at pH 4.0. Fusant F3 was further treated by UV irradiation to generate a mutant, FM1, with improvements in acid tolerance which produced five-fold more lactic acid than the parent strain at acidic pH. The molecular studies using RAPD markers demonstrated that the fusant is derived from both the parental strains, Acetobacter and Lactobacillus and the mutant is derived from the fusant. The utilization of such acid tolerant strains could be able to produce free lactic acid at acidic pH without using neutralizing agents and will offer an effective means for designing environmentally benign processes for lactic acid production.</p>1 aSinghvi, Mamata1 aGurjar, Gayatri1 aGupta, Vidya1 aGokhale, Digambar, V.  uhttps://library.ncl.res.in/content/biocatalyst-development-lactic-acid-production-acidic-ph-using-inter-generic-protoplast-0