02365nas a2200301 4500008004100000022001400041245011000055210006900165260000800234300001200242490000700254520141200261653001301673653001001686653001601696653002301712653002101735653001501756653001701771100001601788700002601804700002501830700002101855700002401876700002001900700002001920856012301940 2020 eng d a1073-608500aDifferential modulation in metabolites revealed with the improvement in the shelf-life of alphonso fruits0 aDifferential modulation in metabolites revealed with the improve cOCT a508-5200 v623 a

Globally farmers have difficulty in extending the shelf-life of the tropical fruits due to their perishable nature. The present study aimed to assess the effect of hexanal nano-formulation treatment (NFT) on the shelf-life of Alphonso mango. Further, volatilomics was performed to explore the molecular basis of such effect. Untreated and treated fruits were sampled starting from 5th to 21st day after NFT at an interval of 4 days. Moderate changes in visual and digital colour parameters were evident from the intact and dissected fruits of NFT set compared to untreated fruits. Biochemical assays affirmed the phenotypic differences with significant changes in the colour imparting compounds like carotenoids and anthocyanins among them. Further, gas chromatography-mass spectrometry analysis revealed significant qualitative and quantitative variations in the different classes of compounds like lactones, furanones, esters, aldehydes and alcohols. Some of the key metabolites showed differential modulations among the NFT and untreated fruit sets indicating their potential role in various processes, which ultimately might have resulted in delayed ripening of the mango. Overall, this study has demonstrated the beneficial effect of hexanal and identified important metabolites with the enhanced shelf-life in Alphonso that could be useful for farmers and mango-based food/flavour industries.

10aAlphonso10aGC-MS10aMetabolites10aNano-biotechnology10aNano-formulation10aShelf-life10aVolatilomics1 aDar, Saleem1 aDholakia, Bhushan, B.1 aShanmugam, Haripriya1 aGupta, Vidya, S.1 aSubramanian, K., S.1 aSubramanian, J.1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/differential-modulation-metabolites-revealed-improvement-shelf-life-alphonso-fruits02347nas a2200265 4500008004100000022001400041245014700055210006900202260000800271300001100279490000800290520144700298653002101745653001201766653001601778653001301794653001901807653001401826100002301840700002701863700002501890700002001915700002001935856012601955 2022 eng d a0168-945200aDietary influence on modulation of Helicoverpa armigera oral secretion composition leading to differential regulation of tomato plant defense.0 aDietary influence on modulation of Helicoverpa armigera oral sec cJAN a1111200 v3143 a

Little is known about how different plant-based diets influence the insect herbivores' oral secretion (OS) composition and eventually the plant defense responses. We analyzed the OS composition of the generalist Lepidopteran insect, Helicoverpa armigera feeding on the host plant tomato (OSH), non-host plant capsicum (OSNH), and artificial diet (OSAD) using Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry. Higher numbers and levels of alkaloids and terpenoids were observed in OSH and OSNH, respectively while OSAD was rich in phospholipids. Interestingly, treatment of H. armigera OSAD, OSH and OSNH on wounded tomato leaves showed differential expression of (i) genes involved in JA and SA biosynthesis and their responsive genes, and (ii) biosynthetic pathway genes of chlorogenic acid (CGA) and trehalose, which exhibited increased accumulation along with several other plant defensive metabolites. Specifically, high levels of CGA were detected after OSH and OSNH treatments in tomato leaves. There was higher expression of the genes involved in phenyl-propanoid biosynthesis, which may lead to the increased accumulation of CGA and related metabolites. In the insect bioassay, CGA significantly inhibited H. armigera larval growth. Our results underline the differential accumulation of plant and insect OS metabolites and identified potential plant metabolite(s) affecting insect growth and development.

10aChlorogenic acid10adefense10aMetabolites10aNon-host10aOral secretion10atrehalose1 aKallure, Gopal, S.1 aShinde, Balkrishna, A.1 aBarvkar, Vitthal, T.1 aKumari, Archana1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/dietary-influence-modulation-helicoverpa-armigera-oral-secretion-composition-leading-002666nas a2200241 4500008004100000022001400041245009700055210006900152260000800221300001100229490000800240520185700248653002502105653002002130653002002150653002602170653001602196100002302212700002802235700002002263700001902283856012202302 2025 eng d a0960-741200aNavigating the challenges of engineering composite specialized metabolite pathways in plants0 aNavigating the challenges of engineering composite specialized m cMAR ae701000 v1213 a

Plants are a valuable source of diverse specialized metabolites with numerous applications. However, these compounds are often produced in limited quantities, particularly under unfavorable ecological conditions. To achieve sufficient levels of target metabolites, alternative strategies such as pathway engineering in heterologous systems like microbes (e.g., bacteria and fungi) or cell-free systems can be employed. Another approach is plant engineering, which aims to either enhance the native production in the original plant or reconstruct the target pathway in a model plant system. Although increasing metabolite production in the native plant is a promising strategy, these source plants are often exotic and pose significant challenges for genetic manipulation. Effective pathway engineering requires comprehensive prior knowledge of the genes and enzymes involved, as well as the precursor, intermediate, branching, and final metabolites. Thus, a thorough elucidation of the biosynthetic pathway is closely linked to successful metabolic engineering in host or model systems. In this review, we highlight recent advances in strategies for biosynthetic pathway elucidation and metabolic engineering. We focus on efforts to engineer complex, multi-step pathways that require the expression of at least eight genes for transient and three genes for stable transformation. Reports on the engineering of complex pathways in stably transformed plants remain relatively scarce. We discuss the major hurdles in pathway elucidation and strategies for overcoming them, followed by an overview of achievements, challenges, and solutions in pathway reconstitution through metabolic engineering. Recent advances including computer-based predictions offer valuable platforms for the sustainable production of specialized metabolites in plants.

10aBiosynthetic pathway10aEnzyme activity10aGene expression10aMetabolic engineering10aMetabolites1 aGharat, Sachin, A.1 aTamhane, Vaijayanti, A.1 aGiri, Ashok, P.1 aAharoni, Asaph uhttps://library.ncl.res.in/content/navigating-challenges-engineering-composite-specialized-metabolite-pathways-plants