02661nas a2200241   4500008004100000022001400041245022700055210006900282260000900351520169200360653002002052653001002072653002202082653002602104653001902130653002602149100001802175700002602193700002902219700002402248700002002272856012702292       2020                        eng d  a0721-759500aCombinatorial approach through in vitro regeneration and phytochemical profiling of ceropegia media (huber) ans.: a potential way forward in the conservation of an endangered medicinal plant from the western ghats in India0 aCombinatorial approach through in vitro regeneration and phytoch  cJUN 3 a<p>Ceropegia mediais an endemic and endangered plant as its propagation through seeds is unreliable due to low germination, slow growth and seedling decay under natural conditions. Also, tubers of this plant are edible serving as carbohydrate source with medicinal values leading to severe population decline in the natural habitat. To provide a sustainable solution, an efficient in vitro propagation protocol along with phytochemical profiling was developed forC. media. Callus cultures were induced from seedling and wild leaf tissues using the most effective Murashige and Skoog's (MS) medium with 2,4-dichlorophenoxyacetic acid (2,4-D; 2 mu M) and sucrose (3%). Somatic embryos were acquired on MS medium with 1 mu M 6-Benzylaminopurine (BAP) and 1 mu M 2,4-D. Conversion into plantlets was attained only from tissue culture-derived seedling leaf (TCDSL) explant. Further, in vitro tuberization was achieved from TCDSL callus with BAP and Naphthalene acetic acid (NAA). AgNO(3)as an elicitor had a positive effect on both fresh and dry weights of callus. Successful acclimatization (58%) was attained after two months resulting in normal phenotype in pots. Further, metabolite profiles of ten different tissues from wild and in vitro plants were compared. Total 82 compounds comprising alkaloids, fatty acids, fatty acid ester, steroids, terpenes and hydrocarbons were identified. Overall, results suggested enhanced production of selected metabolites with in vitro propagation and AgNO3, alleviating the problem of unavailability of planting materials. Thus, the current study might offer potential ways for the conservation of such RED enlisted species asC. media. [GRAHICS]</p>
10aCeropegia media10aGC-MS10aMicrotuberization10aSecondary metabolites10aSilver nitrate10asomatic embryogenesis1 aPandey, Meena1 aDholakia, Bhushan, B.1 aJayaramaiah, Ramesha, H.1 aPunekar, Sachin, A.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/combinatorial-approach-through-vitro-regeneration-and-phytochemical-profiling-ceropegia02001nas a2200253   4500008004100000022001400041245012600055210006900181260000800250300001100258490000800269520115500277653001401432653001201446653001601458653001601474653001401490653002601504100002401530700002201554700002901576700002001605856012201625       2025                        eng d  a0141-813000aMYB proteins: Versatile regulators of plant development, stress responses, and secondary metabolite biosynthetic pathways0 aMYB proteins Versatile regulators of plant development stress re  cFEB  a1385880 v2883 a<p>
	MYB proteins are ubiquitous in nature, regulating key aspects of plant growth and development. Although MYB proteins are known for regulating genes involved in secondary metabolite biosynthesis, particularly phenylpropanoids, their roles in terpenoid, glucosinolate, and alkaloid biosynthesis remain less understood. This review explores the structural and functional differences between activator and repressor MYB proteins along with their roles in plant growth, development, stress responses, and secondary metabolite production. MYB proteins serve as central hubs in protein-protein interaction networks that regulate expression of numerous genes involved in the adaptation of plants to varying environmental conditions. Thus, we also highlight key interacting partners of MYB proteins and their roles in these adaptation mechanisms. We further discuss the mechanisms regulating MYB proteins, including autoregulation, epigenetics, and post-transcriptional and post-translational modifications. Overall, we propose MYB proteins as versatile regulators for improving plant traits, stress responses, and secondary metabolite production.</p>
10aActivator10adefense10aDevelopment10aMYB protein10aRepressor10aSecondary metabolites1 aBhatt, Preshita, A.1 aGurav, Tanuja, P.1 aKondhare, Kirtikumar, R.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/myb-proteins-versatile-regulators-plant-development-stress-responses-and-secondary