02702nas a2200265   4500008004100000022001400041245010900055210006900164260000800233300001400241490000700255520179800262653003302060653002602093653001402119653003002133653001402163100002602177700002402203700001402227700002202241700002302263700002002286856013002306       2025                        eng d  a0965-040700aNigericin-induced apoptosis in acute myeloid leukemia via mitochondrial dysfunction and oxidative stress0 aNigericininduced apoptosis in acute myeloid leukemia via mitocho  cAUG  a2161-21740 v333 a<p>
	Background: Acute Myeloid Leukemia (AML) is a highly aggressive clonal hematological malignancy with limited treatment options. This study aimed to evaluate the therapeutic potential of nigericin, a polyether ionophore derived from Streptomyces DASNCL-29, as a mitochondrial-targeted agent for AML treatment. Methods: Nigericin was isolated from Streptomyces DASNCL-29 and characterized via chromatography and NMR. Its cytotoxicity was tested in MOLM13 (sensitive and venetoclax-resistant) and HL60 (sensitive and cytarabine-resistant) cells using the MTT assay. Mitochondrial dysfunction was assessed by measuring reactive oxygen species (ROS), mitochondrial membrane potential (Arum), and mitochondrial mass. Apoptosis was evaluated with Annexin V/PI assays and immunoblotting, while proteomic analysis was conducted using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) to identify differentially regulated proteins. Results: Nigericin demonstrated potent cytotoxicity with IC50 values of 57.02 nM in MOLM13-sensitive, 35.29 nM in MOLM13-resistant, 20.49 nM in HL60-sensitive, and 1.197 nM in HL60-cytarabine-resistant cells. Apoptosis was confirmed by Annexin V/PI staining and caspase-3/PARP cleavage, along with MCL-1 downregulation. Mitochondrial dysfunction was evident from increased ROS, reduced Arum, and decreased mitochondrial mass. Proteomic profiling identified 264 dysregulated proteins, including a 3.8-fold upregulation of Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit A (SDHA). Conclusion: Nigericin induces apoptosis in AML cells by disrupting mitochondrial function and enhancing oxidative stress. Its nanomolar potency highlights the need for further mechanistic studies and in vivo evaluations to explore its potential in AML treatment.</p>
10aAcute myeloid leukemia (AML)10aAntineoplastic agents10aApoptosis10aMitochondrial dysfunction10aNigericin1 aArun, Bhavyadharshini1 aGopinath, Prarthana1 aJha, Anup1 aTripathi, Nishtha1 aDastager, Syed, G.1 aHasan, Syed, K.  uhttps://library.ncl.res.in/content/nigericin-induced-apoptosis-acute-myeloid-leukemia-mitochondrial-dysfunction-and-oxidative