01918nas a2200205   4500008004100000022001400041245011000055210006900165260000800234300001200242490000700254520118700261100002701448700002201475700002101497700002801518700002301546700002401569856011901593       2025                        eng d  a1525-779700aVat-based 3D-bioprinted scaffolds from photocurable bacterial levan for osteogenesis and immunomodulation0 aVatbased 3Dbioprinted scaffolds from photocurable bacterial leva  cJAN  a954-9660 v263 a<p>
	Emerging techniques of additive manufacturing, such as vat-based three-dimensional (3D) bioprinting, offer novel routes to prepare personalized scaffolds of complex geometries. However, there is a need to develop bioinks suitable for clinical translation. This study explored the potential of bacterial-sourced methacrylate levan (LeMA) as a bioink for the digital light processing (DLP) 3D bioprinting of bone tissue scaffolds. LeMA was successfully synthesized, characterized, and used to fabricate 3D-bioprinted scaffolds with excellent printability and physicochemical properties. In vitro studies demonstrated superior cytocompatibility of 15% w/v LeMA gels compared to 20% gels. 15% LeMA gels supported osteogenic differentiation , as evidenced by alkaline phosphatase activity and mineral deposition by MC3T3 pre-osteoblasts. Importantly, the LeMA hydrogels positively modulated the macrophage phenotype, promoting the expression of the anti-inflammatory marker CD206. These findings suggest that 3D-printed LeMA scaffolds can create a favorable microenvironment for bone regeneration, highlighting their potential for tissue repair and regeneration applications.</p>
1 aWaidi, Yusuf, Olatunji1 aWagh, Vasudev, S.1 aMishra, Shivangi1 aJhunjhunwala, Siddharth1 aDastager, Syed, G.1 aChatterjee, Kaushik  uhttps://library.ncl.res.in/content/vat-based-3d-bioprinted-scaffolds-photocurable-bacterial-levan-osteogenesis-and