04223nas a2200205 4500008004100000245009500041210006900136260000800205520345300213100002203666700002503688700002603713700002503739700002803764700002203792700002303814700002903837700002903866856012203895 2024 eng d00aMultifunctional metabolites of Streptomyces kunmingensis BS19 from bamboo rhizosphere soil0 aMultifunctional metabolites of Streptomyces kunmingensis BS19 fr cNOV3 a

A pigmented bioactive molecule from Streptomyces kunmingensis BS19 was isolated, characterized, and evaluated for anti-infective, antiproliferative, and wound-healing properties. The yellow–red pigment produced from the strain BS19 showed promising activity against methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis. Extracellular bioactive pigment from the strain BS19 was produced by agar surface fermentation and purified through bioassay guided preparative HPLC-based purification. Based on the results of UV, FT-IR, GC–MS, 1H NMR, and 13C NMR spectral analyses, the purified pigment was identified as a chromopeptide class of molecule with phenoxazinone chromophore. Its molecular weight was determined as the chemical formula C64H90N12O16 and molecular weight 1283 g/mol. It exhibited promising antimicrobial activity against Staphylococcus aureus (ATCC 1720) and antiproliferative activity against 14 types of human cancer cell lines. It showed good in vivo wound-healing activity in the rat model. The present study explored Streptomyces kunmingensis as a newly added source for the isolation of chromopeptide antibiotics for antimicrobial, wound-healing, and anticancer applications.

1 aSangeetha, Murthy1 aSasirekha, Sivakumar1 aMahendran, Jaganathan1 aSivarajan, Anbalmani1 aRadhakrishnan, Manikkam1 aManigundan, Kaari1 aDastager, Syed, G.1 aSengottuvelu, Singaravel1 aBalagurunathan, Ramasamy uhttps://library.ncl.res.in/content/multifunctional-metabolites-streptomyces-kunmingensis-bs19-bamboo-rhizosphere-soil