03679nas a2200205 4500008004100000245009500041210007100136260000800207300001400215490000600229520297300235100001903208700001903227700002203246700002403268700001703292700001703309700002103326856012603347 2024 eng d00aphotoarchitectonic hydrogel for synergistic in vitro chemo–phototherapy of breast cancer0 aphotoarchitectonic hydrogel for synergistic in vitro chemo–photo cJAN a1903-19160 v53 a

Currently, combinatorial therapy has evoked interest in cancer treatment, and may promote achieving a synergistic effect using cancer medicines. Laser-assisted and pH-responsive therapies have attracted significant attention, and their combination leads to high efficiency cancer treatment. Herein, we developed a chemo–phototherapeutic hydrogel comprised of doxorubicin (DOX, a chemotherapeutic drug) and zinc phthalocyanine (ZnPc, a phototherapeutic drug) for combinatorial and synergistic treatment of breast cancer. Firstly, we have developed carbon dots (CDs, size of ∼5 nm) utilizing lignin and folic acid as biocompatible sources. Then doxorubicin was loaded on the surface of the carbon dots via conjugation (DOX@CDs). Later on, zinc phthalocyanine and acrylic acid derivatives were utilized to develop a laser-responsive hydrogel (ZnPc-PP H). Afterward, doxorubicin-conjugated carbon dots were incorporated into the photoarchitectonic hydrogel to develop a chemo–phototherapeutic drug-loaded hydrogel (DOX@CDs–ZnPc-PP H). Subsequently, the in vitro pH-triggering experiments demonstrated that the hydrogel loaded with both DOX and ZnPc could release the drugs in an acidic environment. Interestingly, in vitro assays confirmed that DOX@CDs–ZnPc-PP H could effectively target breast cancer cells (MCF-7). Furthermore, the developed chemo–phototherapeutic hydrogel exhibited non-cytotoxic behavior. Owing to laser assisted reactive oxygen species generation from ZnPc present in the hydrogel, the growth of MCF-7 cells was significantly lowered. In conclusion, all experimental outcomes indicate that the photoarchitectonic hydrogel has the potential to be applied in synergistic chemo- and photodynamic therapy of cancer.

1 aPaul, Shatabdi1 aYadav, Binduma1 aPatil, Mahesh, D.1 aPujari, Anil, Kumar1 aSingh, Umesh1 aRishi, Vikas1 aBhaumik, Jayeeta uhttps://library.ncl.res.in/content/photoarchitectonic-hydrogel-synergistic-vitro-chemo%E2%80%93phototherapy-breast-cancer03438nas a2200181 4500008004100000245011500041210006900156260000800225300001400233490000600247520276100253100001803014700003103032700001903063700002203082700002103104856013103125 2025 eng d00aImmobilization of aldehyde reductase for the production of bioplastic precursors from agricultural fatty acids0 aImmobilization of aldehyde reductase for the production of biopl cJUL a3910-39140 v33 a
Herein, we report the biosynthesis of bioplastic precursors thourgh the immobilization of aldehyde reductase (AHR) onto a metal organic framework (UIO-66-NH2). Kinetic analysis demonstrated that the immobilized AHR maintained significant catalytic activity and exhibited improved operational stability, as well as higher reusability, compared with the free AHR. Furthermore, the synthetic applicability of the immobilized AHR was evaluated in tandem with that of transaminase derived from S. pomeroyi (spTA), where 8, 10, and 12 carbon chain ω-amino fatty acids (ω-AFAs) were biosynthesized from the corresponding hydroxy fatty acids (ω-HFAs) with ∼90% conversions.
Herein, we report the biosynthesis of bioplastic precursors via the immobilization of aldehyde reductase (AHR) onto a metal organic framework (UIO-66-NH2).

 

1 aMohne, Devesh1 aReddy, Yeddula, Nikhileshw1 aRawat, Kshitij1 aPatil, Mahesh, D.1 aBhaumik, Jayeeta uhttps://library.ncl.res.in/content/immobilization-aldehyde-reductase-production-bioplastic-precursors-agricultural-fatty-acids