01137nas a2200205   4500008004100000022001400041245009200055210006900147260011000216300001400326490000700340520032800347100001800675700001500693700002000708700002200728700003700750700002500787856011900812       2015                        eng d  a1359-734500aSupramolecular scaffolds on glass slides as sugar based rewritable sensors for bacteria0 aSupramolecular scaffolds on glass slides as sugar based rewritab  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcMAR  a6346-63490 v513 a<p>We describe here the sugar functionalized beta-cyclodextrin-ferrocene glass slides as fully reversible bacterial biosensors under the influence of external adamantane carboxylic acid. The prototype D-mannose - E. coli ORN 178 and L-fucose - P. aeruginosa interactions serve as a model to illustrate the new approach.</p>1 aGade, Madhuri1 aPaul, Ajay1 aAlex, Catherine1 aChoudhury, Devika1 aThulasiram, Hirekodathakallu, V.1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/supramolecular-scaffolds-glass-slides-sugar-based-rewritable-sensors-bacteria-001804nas a2200217   4500008004100000022001400041245010000055210006900155260011000224300001400334490000800348520093200356100001801288700002301306700002701329700002701356700003401383700001901417700002501436856012501461       2016                        eng d  a0003-265400aImmobilization of multivalent glycoprobes on gold surfaces for sensing proteins and macrophages0 aImmobilization of multivalent glycoprobes on gold surfaces for s  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcFEB  a2250-22580 v1413 a<p>The multivalent display of carbohydrates on the cell surface provides cooperative binding to improve the specific biological events. In addition to multivalency, the spatial arrangement and orientation of sugars with respect to external stimuli also trigger carbohydrate-protein interactions. Herein, we report a non-covalent host-guest strategy to immobilize heptavalent glyco-beta-cyclodextrin on gold-coated glass slides to study multivalent carbohydrate-protein interactions. We have found that the localization of sugar entities on surfaces using beta-cyclodextrin (beta-CD) chemistry increased the avidity of carbohydrate-protein and carbohydrate-macrophage interactions compared to monovalent-beta-CD sugar coated surfaces. This platform is expected to be a promising tool to amplify the avidity of sugar-mediated interactions on surfaces and contribute to the development of next generation bio-medical products.</p>1 aGade, Madhuri1 aKhandelwal, Puneet1 aSangabathuni, Sivakoti1 aBavireddi, Harikrishna1 aMurthy, Raghavendra, Vasudeva1 aPoddar, Pankaj1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/immobilization-multivalent-glycoprobes-gold-surfaces-sensing-proteins-and-macrophages01209nas a2200241   4500008004100000022001400041245010700055210006900162260000800231300001400239490000600253520036900259653002700628653001700655653001200672653001500684653003100699100001800730700003200748700003700780700002500817856012500842       2017                        eng d  a2365-654900aEngineering cell surface glycans with carbohydrate enantiomers to alter bacterial binding and adhesion0 aEngineering cell surface glycans with carbohydrate enantiomers t  cSEP  a8865-88690 v23 a<p>Chirality of carbohydrate has critical functions in many biological processes. Changes in the configuration of even one sugar molecules may cause abnormal behavior or even inhibit specific processes. Herein, we have shown bioorthogonal conjugation of mannose enantiomers on HeLa cell surfaces induced a different rate of bacterial binding and cell adhesion.</p>10aBioorthogonal reaction10aCarbohydrate10aE. coli10aEnantiomer10aSurfaces functionalization1 aGade, Madhuri1 aChaudhary, Preeti, Madhukar1 aThulasiram, Hirekodathakallu, V.1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/engineering-cell-surface-glycans-carbohydrate-enantiomers-alter-bacterial-binding-and01489nas a2200181   4500008004100000245013100041210006900172260000800241300001400249490000700263520078300270100002001053700001801073700002701091700003701118700002501155856012701180       2017                        eng d00aExploring the influence of shapes and heterogeneity of glyco-gold nanoparticles on bacterial binding for preventing infections0 aExploring the influence of shapes and heterogeneity of glycogold  cJUL  a1116-11240 v123 aTo investigate the effects of the heterogeneity and shape of glyco-nanoprobes on carbohydrate-protein interactions (CPIs), alpha-d-mannose-and beta-d-galactose-linked homo-and heterogeneous glycodendrons were synthesized and immobilized on spherical and rod-shaped gold nanoparticles (AuNPs). Lectin and bacterial binding studies of these glyco-AuNPs clearly illustrate that multivalency and shape of AuNPs contribute significantly to CPIs than the heterogeneity of glycodendrons. Finally, bacterial infection of HeLa cells was effectively inhibited by the homogeneous glycodendron-conjugated rod-shaped AuNPs relative to their heterogeneous counterparts. Overall, these results provide insight into the role of AuNP shape and multivalency as potential factors to regulate CPIs.1 aToraskar, Suraj1 aGade, Madhuri1 aSangabathuni, Sivakoti1 aThulasiram, Hirekodathakallu, V.1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/exploring-influence-shapes-and-heterogeneity-glyco-gold-nanoparticles-bacterial-binding