02670nas a2200361   4500008004100000022001400041245023500055210007000290260000800360300001000368490000800378520138500386653002601771653002601797653001501823653002501838653001501863653001501878100001701893700001701910700002401927700002001951700001801971700001801989700002702007700003102034700003002065700002002095700001802115700001602133700002502149856013402174       2024                        eng d  a0168-165600aSustainable bioprocess technology for producing food-flavour (+)-γ-decalactone from castor oil-derived ricinoleic acid using enzymatic activity of Candida parapsilosis: Scale-up optimization and purification using novel composite0 aSustainable bioprocess technology for producing foodflavour γdec  cSEP  a17-300 v3933 a<p>
	Ricinoleic acid (RA) from castor oil was employed in biotransformation of peach-flavoured gamma-decalactone (GDL), using a Candida parapsilosis strain (MTCC13027) which was isolated from waste of pineapple crown base. Using four variables-pH, cell density, amount of RA, and temperature-the biotransformation parameters were optimized using RSM and BBD. Under optimized conditions (pH 6, 10 % of microbial cells, 10 g/L RA at 28 degrees C), the conversion was maximum and resulted to 80 % (+)-GDL (4.4 g/L/120 h) yield in shake flask (500 mL). Furthermore, optimization was achieved by adjusting the aeration and agitation parameters in a 3 L bioreactor, which were then replicated in a 10 L bioreactor to accurately determine the amount of (+)-GDL. In bioreactor condition, 4.7 g/L (&gt;85 %) of (+)-GDL is produced with 20 % and 40 % dissolved oxygen (1.0 vvm) at 150 rpm in 72 h and 66 h, respectively. Further, a new Al-Mg-Ca-Si composite column-chromatography method is developed to purify enantiospecific (+)-GDL (99.9 %). This (+)-GDL is 100 % nature-identical as validated through 14C-radio-carbon dating. Thorough chemical investigation of enantiospecific (+)-GDL is authenticated for its use as flavour. This bioflavour has been developed through a cost-effective biotechnological process in response to the demand from the food industry on commercial scale.</p>
10a(+)-gamma-Decalactone10aAl-Mg-Ca-Si composite10aBioflavour10aCandida parapsilosis10acastor oil10aFood-waste1 aSyed, Naziya1 aSingh, Suman1 aChaturvedi, Shivani1 aKumar, Prashant1 aKumar, Deepak1 aJain, Abhinav1 aSharma, Praveen, Kumar1 aNannaware, Ashween, Deepak1 aChanotiya, Chandan, Singh1 aBhambure, Rahul1 aKumar, Pankaj1 aKalra, Alok1 aRout, Prasant, Kumar  uhttps://library.ncl.res.in/content/sustainable-bioprocess-technology-producing-food-flavour-%CE%B3-decalactone-castor-oil-derived