01085nas a2200349 4500008004100000245009600041210006900137260000900206490000700215653001200222653002300234653001200257653002400269653001600293100001700309700001600326700002700342700002000369700002200389700001800411700001800429700001700447700001900464700001700483700001900500700002200519700001800541700002000559700001900579700001800598856011900616 2024 eng d00aBiocatalysis enables the scalable conversion of biobased furans into various furfurylamines0 aBiocatalysis enables the scalable conversion of biobased furans  cJUL 0 v1510aBiomass10aOMEGA-TRANSAMINASE10aOXIDASE10aReductive amination10aSPECIFICITY1 aGiri, Pritam1 aLim, Seonga1 aKhobragade, Taresh, P.1 aPagar, Amol, D.1 aPatil, Mahesh, D.1 aSarak, Sharad1 aJeon, Hyunwoo1 aJoo, Sangwoo1 aGoh, Younghwan1 aJung, Seohee1 aJang, Yu-Jeong1 aChoi, Seung, Beom1 aKim, Ye, Chan1 aKang, Taek, Jin1 aHeo, Yong-Seok1 aYun, Hyungdon uhttps://library.ncl.res.in/content/biocatalysis-enables-scalable-conversion-biobased-furans-various-furfurylamines03679nas a2200205 4500008004100000245009500041210007100136260000800207300001400215490000600229520297300235100001903208700001903227700002203246700002403268700001703292700001703309700002103326856012603347 2024 eng d00aphotoarchitectonic hydrogel for synergistic in vitro chemo–phototherapy of breast cancer0 aphotoarchitectonic hydrogel for synergistic in vitro chemo–photo cJAN a1903-19160 v53 a

Currently, combinatorial therapy has evoked interest in cancer treatment, and may promote achieving a synergistic effect using cancer medicines. Laser-assisted and pH-responsive therapies have attracted significant attention, and their combination leads to high efficiency cancer treatment. Herein, we developed a chemo–phototherapeutic hydrogel comprised of doxorubicin (DOX, a chemotherapeutic drug) and zinc phthalocyanine (ZnPc, a phototherapeutic drug) for combinatorial and synergistic treatment of breast cancer. Firstly, we have developed carbon dots (CDs, size of ∼5 nm) utilizing lignin and folic acid as biocompatible sources. Then doxorubicin was loaded on the surface of the carbon dots via conjugation (DOX@CDs). Later on, zinc phthalocyanine and acrylic acid derivatives were utilized to develop a laser-responsive hydrogel (ZnPc-PP H). Afterward, doxorubicin-conjugated carbon dots were incorporated into the photoarchitectonic hydrogel to develop a chemo–phototherapeutic drug-loaded hydrogel (DOX@CDs–ZnPc-PP H). Subsequently, the in vitro pH-triggering experiments demonstrated that the hydrogel loaded with both DOX and ZnPc could release the drugs in an acidic environment. Interestingly, in vitro assays confirmed that DOX@CDs–ZnPc-PP H could effectively target breast cancer cells (MCF-7). Furthermore, the developed chemo–phototherapeutic hydrogel exhibited non-cytotoxic behavior. Owing to laser assisted reactive oxygen species generation from ZnPc present in the hydrogel, the growth of MCF-7 cells was significantly lowered. In conclusion, all experimental outcomes indicate that the photoarchitectonic hydrogel has the potential to be applied in synergistic chemo- and photodynamic therapy of cancer.

1 aPaul, Shatabdi1 aYadav, Binduma1 aPatil, Mahesh, D.1 aPujari, Anil, Kumar1 aSingh, Umesh1 aRishi, Vikas1 aBhaumik, Jayeeta uhttps://library.ncl.res.in/content/photoarchitectonic-hydrogel-synergistic-vitro-chemo%E2%80%93phototherapy-breast-cancer03438nas a2200181 4500008004100000245011500041210006900156260000800225300001400233490000600247520276100253100001803014700003103032700001903063700002203082700002103104856013103125 2025 eng d00aImmobilization of aldehyde reductase for the production of bioplastic precursors from agricultural fatty acids0 aImmobilization of aldehyde reductase for the production of biopl cJUL a3910-39140 v33 a
Herein, we report the biosynthesis of bioplastic precursors thourgh the immobilization of aldehyde reductase (AHR) onto a metal organic framework (UIO-66-NH2). Kinetic analysis demonstrated that the immobilized AHR maintained significant catalytic activity and exhibited improved operational stability, as well as higher reusability, compared with the free AHR. Furthermore, the synthetic applicability of the immobilized AHR was evaluated in tandem with that of transaminase derived from S. pomeroyi (spTA), where 8, 10, and 12 carbon chain ω-amino fatty acids (ω-AFAs) were biosynthesized from the corresponding hydroxy fatty acids (ω-HFAs) with ∼90% conversions.
Herein, we report the biosynthesis of bioplastic precursors via the immobilization of aldehyde reductase (AHR) onto a metal organic framework (UIO-66-NH2).

 

1 aMohne, Devesh1 aReddy, Yeddula, Nikhileshw1 aRawat, Kshitij1 aPatil, Mahesh, D.1 aBhaumik, Jayeeta uhttps://library.ncl.res.in/content/immobilization-aldehyde-reductase-production-bioplastic-precursors-agricultural-fatty-acids02469nas a2200361 4500008004100000022001400041245013600055210006900191260000900260490000700269520131800276653001801594653003001612653001701642653001801659100002701677700001701704700002201721700001701743700001501760700001601775700001701791700001901808700002001827700001701847700002001864700001701884700002001901700002001921700001901941700001801960856012901978 2025 eng d a1433-785100aTotal biocatalytic synthesis of capsaicinoids using ferulic acid: a versatile two-step strategy for natural product diversification0 aTotal biocatalytic synthesis of capsaicinoids using ferulic acid cDEC 0 v643 a

The wide-ranging application of capsaicinoids, the active compounds in chili peppers, has driven increasing interest in the development of sustainable production strategies. However, capsaicinoid synthesis remains a challenge. The objective of this pioneering study is to report the total biocatalytic synthesis of structurally diverse capsaicinoids from bio-based ferulic acids. An X-ray crystallographic study elucidated the structural basis for the exceptional potential of a novel transaminase from Phaeobacter porticola (PPTA) to transform the highest ever reported concentration of vanillin (100-200 mM) to vanillylamine, with >99% conversion and modest conversion ranging from 48% to 79% for 300 to 500 mM substrate. Using PPTA in tandem with phenolic acid decarboxylase (PAD) and aromatic dioxygenase (ADO) further enabled the direct synthesis of vanillylamine from ferulic acid with >99% conversion. Furthermore, the integration of a multi-enzymatic cascade with carboxylic acid reductases (CARs) successfully synthesized structurally diverse capsaicinoids via amide bond formation between vanillylamine and free fatty acids, with excellent conversions ranging from 72% to >88%. A 50-mM enzymatic reaction afforded 95% and 80% conversion of vanillylamine and capsaicin, respectively.

10aCapsaicinoids10aCarboxylic acid reductase10aTransaminase10aVanillylamine1 aKhobragade, Taresh, P.1 aGiri, Pritam1 aPatil, Mahesh, D.1 aJoo, Sangwoo1 aCho, Sunga1 aKim, Yechan1 aGhosh, Rohan1 aJeong, Sanghun1 aMaeng, Minyeong1 aSong, Min-Ho1 aPark, Jeong-Min1 aLee, Eun, Ho1 aKeum, Young-Soo1 aKang, Taek, Jin1 aHeo, Yong-Seok1 aYun, Hyungdon uhttps://library.ncl.res.in/content/total-biocatalytic-synthesis-capsaicinoids-using-ferulic-acid-versatile-two-step-strategy