02769nas a2200229 4500008004100000245012000041210006900161260000800230300001100238490000800249520195200257653002702209653001502236653003002251653002102281100002102302700001702323700002602340700002002366700002702386856012602413 2024 eng d00aBiodegradable and 3D printable lysine functionalized polycaprolactone scaffolds for tissue engineering applications0 aBiodegradable and 3D printable lysine functionalized polycaprola cMAY a2138160 v1593 a

Tissue engineering (TE) has sparked interest in creating scaffolds with customizable properties and functional bioactive sites. However, due to limitations in medical practices and manufacturing technologies, it is challenging to replicate complex porous frameworks with appropriate architectures and bioactivity in vitro. To address these challenges, herein, we present a green approach that involves the amino acid (L-lysine) initiated polymerization of epsilon-caprolactone (CL) to produce modified polycaprolactone (PCL) with favorable active sites for TE applications. Further, to better understand the effect of morphology and porosity on cell attachment and proliferation, scaffolds of different geometries with uniform and interconnected pores are designed and fabricated, and their properties are evaluated in comparison with commercial PCL. The scaffold morphology and complex internal micro-architecture are imaged by micro-computed tomography (micro-CT), revealing pore size in the range of similar to 300-900 mu m and porosity ranging from 30 to 70 %, while based on the geometry of scaffolds the compressive strength varied from 143 +/- 19 to 214 +/- 10 MPa. Additionally, the degradation profiles of fabricated scaffolds are found to be influenced by both the chemical nature and product design, where Lys-PCL-based scaffolds with better porosity and lower crystallinity degraded faster than commercial PCL scaffolds. According to in vitro studies, Lys-PCL scaffolds have produced an environment that is better for cell adhesion and proliferation. Moreover, the scaffold design affects the way cells interact; Lys-PCL with zigzag geometry has demonstrated superior in vitro vitality (>90 %) and proliferation in comparison to other designs. This study emphasizes the importance of enhancing bioactivity while meeting morphology and porosity requirements in the design of scaffolds for tissue engineering applications.

10aAdditive manufacturing10aAmino acid10aMicro-computed tomography10aPolycaprolactone1 aNaik, Sonali, S.1 aTorris, Arun1 aChoudhury, Namita, R.1 aDutta, Naba, K.1 aNair, Kiran, Sukumaran uhttps://library.ncl.res.in/content/biodegradable-and-3d-printable-lysine-functionalized-polycaprolactone-scaffolds-tissue02681nas a2200265 4500008004100000022001400041245012400055210006900179260000800248300001100256490000800267520176100275653001602036653001602052653002702068653001702095653002702112653002202139653003202161100002102193700002002214700002702234700002702261856012702288 2025 eng d a0001-868600aCritical advances in biofabrication and biomaterial strategies in tracheal tissue engineering: A comprehensive overview0 aCritical advances in biofabrication and biomaterial strategies i cNOV a1036040 v3453 a

The trachea is a vital respiratory organ that connects the larynx to the lungs and performs crucial functions. Various conditions can cause severe and often irreversible damage to individuals trachea of all age groups. Tracheal regeneration remains a major challenge in respiratory medicine, requiring a innovative solutions to address various underlying causes. Existing clinical interventions often have significant limitations and associated complications. Tissue engineering has potential, but its effectiveness has been limited due to challenges such as poor durability and insufficient revascularization. This review aims to provide a comprehensive exploration of the landscape of tracheal regeneration, shedding light on the path towards advancements in addressing extensive tracheal defects. It follows a structured approach, introducing various surgical procedures, along with their associated complications. Subsequently, it delves into the myriad biomaterials investigated in the realm of tracheal tissue engineering, emphasizing the significance of design considerations in scaffold fabrication. The review then navigates through various platforms utilized in tracheal tissue engineering and recent innovative approaches employed in this domain. Additionally, it provides insights into the clinical translation of tissueengineered trachea, highlighting recent advancements and challenges encountered in real-world applications. Finally, it discusses the significant challenges and offers a perspective outlook on the future of tracheal tissue engineering. Addressing current limitations and envisioning novel strategies, the review contributes to the ongoing dialogue and progression in this critical field of regenerative medicine.

10a3D printing10a4D printing10aAdditive manufacturing10aBiomaterials10aCell sheet engineering10aOrganoid printing10aTracheal tissue engineering1 aNaik, Sonali, S.1 aDutta, Naba, K.1 aNair, Kiran, Sukumaran1 aChoudhury, Namita, Roy uhttps://library.ncl.res.in/content/critical-advances-biofabrication-and-biomaterial-strategies-tracheal-tissue-engineering02550nas a2200217 4500008004100000022001400041245011000055210006900165260000900234300001600243490000700259520177100266100002102037700002602058700002402084700002402108700002002132700002702152700002702179856012602206 2025 eng d a2050-750X00aDesign, fabrication and comprehensive testing of biodegradable 3D printable hybrid polymer airway splints0 aDesign fabrication and comprehensive testing of biodegradable 3D cAUG  a10249-102660 v133 a

The trachea plays a critical role in respiration and airway protection but is susceptible to damage from pathological conditions such as stenosis, fistula, obstruction, and malacia. While existing treatment options are useful, they often have limitations, driving the need for innovative alternatives. This study introduces a novel approach using 3D printing technology to create hybrid degradable tracheal splints made of pectin-g-polycaprolactone (pec-g-PCL). We synthesized and characterized various compositions of pec-g-PCL to assess their physicochemical properties and tested their suitability for 3D printing. The resulting materials demonstrated the potential for use as tracheal splints. Using CAD software, we created two distinct designs, which were then fabricated according to those specifications. Micro-computed tomography (micro-CT) imaging revealed splint porosities ranging from 80% to 90%, highlighting their intricate internal microarchitecture. Design verification was conducted through numerical simulations, based on finite element modeling (FEM), to evaluate mechanical properties and computational fluid dynamics (CFD) for assessing the airflow dynamics of the fabricated tracheal splints. Degradation studies indicated that the 3D-printed scaffolds exhibited approximately 30% degradation over a period of 35 days. In vitro, biocompatibility assessments confirmed the scaffold's compatibility with biological systems. These findings demonstrate the potential of pec-g-PCL-based tracheal splints as a promising solution to overcome limitations in current treatments. This research paves the way for advanced biomaterials that could revolutionize patient care by offering more effective solutions for managing tracheal disorders.

1 aNaik, Sonali, S.1 aVongsvivut, Jitraporn1 aDekiwadia, Chaitali1 aAbraham, Amanda, N.1 aDutta, Naba, K.1 aChoudhury, Namita, Roy1 aNair, Kiran, Sukumaran uhttps://library.ncl.res.in/content/design-fabrication-and-comprehensive-testing-biodegradable-3d-printable-hybrid-polymer02613nas a2200229 4500008004100000022001400041245016000055210006900215260000800284300001100292490000700303520173900310653002802049653002102077653003302098653003202131653001902163100002102182700002402203700002702227856012902254 2025 eng d a1042-714700aOptimization and mechanistic insights of zinc ascorbate catalyst for ring-opening polymerization of caprolactone using RSM methodology and DFT calculations0 aOptimization and mechanistic insights of zinc ascorbate catalyst cAPR ae701820 v363 a

Polycaprolactone (PCL) is an aliphatic polyester recognized for its exceptional versatility and biodegradability, which has garnered significant attention for a wide range of applications. This study presents an innovative methodology for the synthesis of PCL through the ring-opening polymerization (ROP) of epsilon-caprolactone (CL). The approach employs a biocompatible and environmentally benign organometallic zinc ascorbate complex as a catalyst. Derived from ascorbic acid, a well-established reducing agent, zinc ascorbate serves as a sustainable alternative to conventional tin-based catalysts, thereby mitigating environmental impact and enhancing safety measures. The catalyst operates effectively under solvent-free conditions and does not require initiators, achieving a high polymer conversion rate of approximately 79%. The optimization of the reaction parameters was conducted using response surface methodology (RSM) employing a central composite design (CCD). The structural and chemical characterization of the catalyst as well as the resulting polymers was performed using proton nuclear magnetic resonance (1H NMR), Fourier-transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analyses. Additionally, density functional theory (DFT) calculations elucidated a four-step coordination-insertion mechanism for the polymerization of cyclic lactones, with findings supported by Gibbs free energy, electrostatic potential, and non-covalent interactions. This study underscores the potential of zinc ascorbate as a reliable, nontoxic, and cost-effective catalyst, fulfilling the increasing demand for sustainable and efficient polymerization processes in commercial and biomedical applications.

10adensity function theory10aPolycaprolactone10aResponse surface methodology10aring-opening polymerization10azinc ascorbate1 aNaik, Sonali, S.1 aVedpathak, Shantanu1 aNair, Kiran, Sukumaran uhttps://library.ncl.res.in/content/optimization-and-mechanistic-insights-zinc-ascorbate-catalyst-ring-opening-polymerization00798nas a2200217 4500008004100000022001400041245010700055210006900162260000800231490000800239653002600247653002700273653002500300100002100325700001700346700002700363700002100390700001600411700002700427856012600454 2025 eng d a1549-329600aThrombin immobilized hemocompatible radiopaque polyurethane microspheres for topical blood coagulation0 aThrombin immobilized hemocompatible radiopaque polyurethane micr cJAN0 v11310aALGINATE MICROSPHERES10aENDOVASCULAR TREATMENT10aPRECISE LOCALIZATION1 aNaik, Sonali, S.1 aTorris, Arun1 aGhuge, Gorakh, Hiraman1 aKarthika, V., K.1 aJoseph, Roy1 aNair, Kiran, Sukumaran uhttps://library.ncl.res.in/content/thrombin-immobilized-hemocompatible-radiopaque-polyurethane-microspheres-topical-blood