01591nas a2200193   4500008004100000022001400041245012600055210006900181260007100250300001200321490000700333520081400340100001901154700002301173700002101196700002301217700002601240856013101266       2014                        eng d  a1043-180200aMonitoring cellular uptake and cytotoxicity of copper(II) complex using a fluorescent anthracene thiosemicarbazone ligand0 aMonitoring cellular uptake and cytotoxicity of copperII complex   a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcJAN  a102-1140 v253 a<p>The thiosemicarbazone derivative of anthracene (ATSC, anthracene thiosemicarbazone I) and its copper(II) complex (CuATSC, 2) were synthesized and characterized by spectroscopic, electrochemical, and crystallographic techniques. Interaction of 1 and 2 with calf thymus (CT) DNA was explored using absorption and emission spectral methods, and viscosity measurements reveal a partial-intercalation binding mode. Their protein binding ability was monitored by the quenching of tryptophan emission using bovine serum albumin (BSA) as a model protein. Furthermore, their cellular uptake, in vitro cytotoxicity testing on the HeLa cell line, and flow cytometric analysis were carried out to ascertain the mode of cell death. Cell cycle analysis indicated that 1 and 2 cause cell cycle arrest in sub-G1 phase.</p>1 aKate, Anup, N.1 aKumbhar, Anupa, A.1 aKhan, Ayesha, A.1 aJoshi, Pranaya, V.1 aPuranik, Vedavati, G.  uhttps://library.ncl.res.in/content/monitoring-cellular-uptake-and-cytotoxicity-copperii-complex-using-fluorescent-anthracene-001777nas a2200241   4500008004100000022001400041245010600055210006900161260011000230300001400340490000700354520084200361100002601203700001901229700001901248700001701267700002401284700002601308700002301334700002601357700002501383856012701408       2015                        eng d  a1144-054600aSynthesis, DNA interaction and anticancer activity of 2-anthryl substituted benzimidazole derivatives0 aSynthesis DNA interaction and anticancer activity of 2anthryl su  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAPR  a4882-48900 v393 a<p>2-Anthryl benzimidazole derivatives (5-7) with hydrogen, carboxyl and benzoyl substituents at the 5th position have been synthesized using a silica supported periodic acid catalyst. The DNA cleavage activity of 5-7 was studied in the presence of light using pBR322 plasmid DNA and was shown to vary with substitution at the 5th position of benzimidazole derivatives. DNA binding studies using ethidium bromide displacement assay demonstrated the non-intercalative binding mode of 5-7. The anticancer activity of these target molecules was tested against MCF-7 and HL-60 cell lines, and they exhibited remarkable activity in the micromolar range. Cellular uptake and morphological changes were confirmed by fluorescence and confocal microscopy. A molecular docking study was carried out to explore the DNA binding mechanism of 5-7.</p>1 aSontakke, Vyankat, A.1 aKate, Anup, N.1 aGhosh, Sougata1 aMore, Piyush1 aGonnade, Rajesh, G.1 aKumbhar, Navanath, M.1 aKumbhar, Anupa, A.1 aChopade, Balu, Ananda1 aShinde, Vaishali, S.  uhttps://library.ncl.res.in/content/synthesis-dna-interaction-and-anticancer-activity-2-anthryl-substituted-benzimidazole-0