02105nas a2200265   4500008004100000022001400041245015200055210006900207260000900276490000800285520115100293653002601444653002201470653001701492653001401509100002701523700002101550700002401571700002501595700002101620700002101641700002101662700002501683856013101708       2023                        eng d  a0223-523400aSynthesis of novel rhodamine type anthrone Spiro-lactam (ASL) analogues and evaluation of antiviral activity against dengue and chikungunya viruses0 aSynthesis of novel rhodamine type anthrone Spirolactam ASL analo  cDEC 0 v2613 a<p>
	A series of Rhodamine type Anthrone-Spirolactam (ASL) derivatives Benzylimin-Anthrone-Spirolactam (ASL-1 to ASL-10) and Benzamide-Anthrone-Spirolactam (ASL-11 and ASL-12) were synthesized via a simple condensation reaction between Anthrone Spiro-lactamine (2) and various aromatic aldehyde and acyl chlorides respectively. Since rhodamine-based compounds were reported to have antiviral activity, the ASL derivatives were examined for in vitro antiviral activity against dengue and chikungunya viruses. Among all the analogues, ASL-3, ASL-6, ASL-7, ASL-8, ASL-9 and ASL-10 were the most potent against dengue virus (DENV) and exerted around one log reduction in virus titre under post -treatment conditions. At the same time ASL-3 was effective under cotreatment conditions. Two analogues ASL-6 and ASL-12 exerted anti-chikungunya virus (CHIKV) activity under post -treatment conditions. In silico docking studies revealed that the ASL derivatives interacted with the proteins of DENV and CHIKV. Together, the results suggest the anti-DENV and CHIKV activity of ASL derivatives which may be exploited further for therapeutic purposes.</p>
10aAnthrone Spiro-lactam10aChikungunya virus10adengue virus10aRhodamine1 aDarole, Ratanamala, S.1 aBagad, Pooja, K.1 aGonnade, Rajesh, G.1 aAlagarasu, Kalichamy1 aPunekar, Madhura1 aShukla, Shridhar1 aParashar, Deepti1 aSenthilkumar, Beeran  uhttps://library.ncl.res.in/content/synthesis-novel-rhodamine-type-anthrone-spiro-lactam-asl-analogues-and-evaluation-antiviral02574nas a2200325   4500008004100000022001400041245010700055210007100162260000900233300001400242490000700256520148700263653003201750653001501782653004001797653003001837653001801867653001701885100002501902700001901927700002101946700002301967700001801990700001402008700002102022700001902043700002502062700002102087856014002108       2025                        eng d  a1756-891900aEffectiveness of 3-amino-2-thiocyanato-α, β-unsaturated carbonyl compounds against chikungunya virus0 aEffectiveness of 3amino2thiocyanatoα βunsaturated carbonyl compo  cJUN   a1269-12790 v173 a<p>Aim Chikungunya fever (CHIKF) caused by the chikungunya virus (CHIKV) is characterized by the presence of long-term polyarthralgia in a minor proportion of the infected patients. Currently, there are no FDA-approved antivirals available. This study evaluated the anti-CHIKV potential of 16 synthetic 3-amino-2-thiocyanato-alpha, beta-unsaturated carbonyl compounds and elucidated their probable mechanisms of action. Methods Anti-CHIKV activity of 16 compounds were investigated in Vero CCL-81 cells using focus forming unit assay (FFU). Dose-dependent and time-dependent antiviral assays were performed for the effective compounds. Molecular docking was performed to find out their interactions with viral proteins. Results Five compounds showed promising anti-CHIKV activity by reducing viral titer with &gt;1 log10 FFU/ml. Dose-dependent studies revealed that the compound 3 g was more effective in reducing the virus titer with a half-maximal inhibitory concentration (IC50) of 0.4315 mu M and a selectivity index of 35.99. Docking analyses revealed that all the compounds mainly interact with the non-structural proteins of CHIKV. Conclusions These findings demonstrate the in vitro anti-CHIKV activity of these compounds, and their possible mode of action via interference with early stages of infection and replication processes. This study warrants further preclinical and clinical evaluation to establish their safety and efficacy as novel anti-CHIKV therapeutics.</p>
10a3-amino-2-thiocyanato-alpha10aantivirals10abeta-unsaturated carbonyl compounds10aChikungunya virus (CHIKV)10ansP2 helicase10atherapeutics1 aAlagarasu, Kalichamy1 aDhote, Radhika1 aBagad, Pooja, K.1 aKharikar, Dwidhesh1 aPatil, Poonam1 aRoy, Diya1 aShukla, Shridhar1 aCherian, Sarah1 aSenthilkumar, Beeran1 aParashar, Deepti  uhttps://library.ncl.res.in/content/effectiveness-3-amino-2-thiocyanato-%CE%B1-%CE%B2-unsaturated-carbonyl-compounds-against-chikungunya