02549nas a2200241   4500008004100000022001400041245016900055210006900224260000800293300001200301490000700313520160600320653002201926653002001948653003801968653003202006653008102038653001202119100001702131700001602148700002202164856012102186       2023                        eng d  a0026-261700aIsolation and biochemical characterization of acid phytase from aspergillus niger and its applications in dephytinization of phytic acid in poultry feed ingredients0 aIsolation and biochemical characterization of acid phytase from   cAPR  a221-2290 v923 a<p>
	Acidic phytase (PHY-B) was isolated from Aspergillus niger BIONCL8 strain, and assessed its application for the dephytinization of poultry feed ingredients. Aspergillus niger BIONCL8 strain was identified by precise molecular methods by targeting internal transcribed spacer (ITS) region of the fungi and molecular detection of PHY gene in the strain was confirmed by sequence analysis with respect to PHY production. The enzyme was isolated and purified by DEAE Sephadex A-50 and Bio-Gel P-60 Gel ion-exchange chromatography. The estimated molecular weight of the protein was 65 kDa on SDS-PAGE, it had a specific activity of 21.18 U/mg. The molecular weight of the protein was confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The peptide sequence of PHY-B was identified using high resolution-mass spectrometry (LC-HR-MS), and its physicochemical parameters were studied. The PHY was stable at acidic pH 2.1, most active at 40 degrees C and was stable up to 80 degrees C, retaining 30% residual activity after 1 h of incubation. The PHY activity was enhanced in the presence of Mg2+ and EDTA, and activity was inhibited in the presence of Hg2+ and K+. The K-m and V-max of PHY were recorded as 3.35 mM and 1.27 U/mg, respectively. The PHY was shown to play a significant role in decreasing PA content in various poultry feed ingredients, ranging from 48.14 to 82.14%. The novel features of the enzyme can be used to decrease the PA content in feed ingredients and increase the bioavailability of nutrients to non-ruminant animals.</p>
10aAspergillus niger10adephytinization10ahigh resolution-mass spectrometry10aion-exchange chromatography10amatrix-assisted laser desorption ionization time-of-flight mass spectrometry10aPhytase1 aBhandari, Y.1 aSonwane, B.1 aVamkudoth, K., R.  uhttps://library.ncl.res.in/content/isolation-and-biochemical-characterization-acid-phytase-aspergillus-niger-and-its