02347nas a2200289   4500008004100000245011900041210006900160260000800229300001100237490000800248520141100256100002101667700001701688700001601705700001801721700002001739700002301759700001901782700001601801700001801817700001401835700002001849700002401869700001901893700001501912856013001927       2022                        eng d00aIdentification and characterization of the key enzyme in the biosynthesis of the neurotoxin beta-ODAP in grass pea0 aIdentification and characterization of the key enzyme in the bio  cMAY  a1018060 v2983 a<p>
	Grass pea (Lathyrus sativus L.) is a grain legume commonly grown in Asia and Africa for food and forage. It is a highly nutritious and robust crop, capable of surviving both droughts and floods. However, it produces a neurotoxic compound, beta-N- oxalyl-L-alpha,beta-diaminopropionic acid (beta-ODAP), which can cause a severe neurological disorder when consumed as a primary diet component. While the catalytic activity associated with beta-ODAP formation was demonstrated more than 50 years ago, the enzyme responsible for this activity has not been identified. Here, we report on the identity, activity, 3D structure, and phylogenesis of this enzyme-beta-ODAP synthase (BOS). We show that BOS belongs to the benzylalcohol O-acetyltransfer-ase, anthocyanin O-hydroxycinnamoyltransferase, anthranilate N-hydroxycinnamoyl/benzoyltransferase, deacetylvindoline 4-O-acetyltransferase superfamily of acyltransferases and is structurally similar to hydroxycinnamoyl transferase. Using molecular docking, we propose a mechanism for its catalytic activity, and using heterologous expression in tobacco leaves (Nicotiana benthamiana), we demonstrate that expression of BOS in the presence of its substrates is sufficient for beta-ODAP production in vivo. The identification of BOS may pave the way toward engineering beta-ODAP-free grass pea cultivars, which are safe for human and animal consumption.</p>
1 aGoldsmith, Moshe1 aBarad, Shiri1 aKnafo, Maor1 aSavidor, Alon1 aBen-Dor, Shifra1 aBrandis, Alexander1 aMehlman, Tevie1 aPeleg, Yoav1 aAlbeck, Shira1 aDym, Orly1 aBen-Zeev, Efrat1 aBarbole, Ranjit, S.1 aAharoni, Asaph1 aReich, Ziv  uhttps://library.ncl.res.in/content/identification-and-characterization-key-enzyme-biosynthesis-neurotoxin-beta-odap-grass-pea