01871nas a2200181   4500008004100000022001400041245015600055210006900211260009100280300001600371490000600387520106700393100002001460700001801480700004301498700001701541856013101558       2013                        eng d  a1932-620300aIsolation, purification and characterization of vinblastine and vincristine from endophytic fungus fusarium oxysporum isolated from catharanthus roseus0 aIsolation purification and characterization of vinblastine and v  a1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USAbPUBLIC LIBRARY SCIENCEcSEP  aUNSP e718050 v83 a<p>Endophytic fungi reside in a symbiotic fashion inside their host plants, mimic their chemistry and interestingly, produce the same natural products as their hosts and are thus being screened for the production of valuable compounds like taxol, camptothecin, podophyllotoxin, etc. Vinblastine and vincristine are excellent anti-cancer drugs but their current production using plants is non-abundant and expensive. In order to make these drugs readily available to the patients at affordable prices, we isolated the endophytic fungi from Catharanthus roseus plant and found a fungus AA-CRL-6 which produces vinblastine and vincristine in appreciable amounts. These drugs were purified by TLC and HPLC and characterized using UV-Vis spectroscopy, ESI-MS, MS/MS and H-1 NMR. One liter of culture filtrate yielded 76 mu g and 67 mu g of vinblastine and vincristine respectively. This endophytic fungal strain was identified as Fusarium oxysporum based upon its cultural and morphological characteristics and internal transcribed spacer (ITS) sequence analysis.</p>1 aKumar, Ashutosh1 aPatil, Deepak1 aRajamohanan, Pattuparambil, Ramanpilla1 aAhmad, Absar  uhttps://library.ncl.res.in/content/isolation-purification-and-characterization-vinblastine-and-vincristine-endophytic-fungus-001933nas a2200253   4500008004100000022001400041245014900055210006900204260000800273300001200281490000700293520097800300653002501278653003801303653002601341653001301367653002301380653003001403100001701433700003001450700004301480700002601523856013001549       2022                        eng d  a0959-810300aInvestigation of domain structures in monomethoxy poly(ethylene glycol)-b-poly(caprolactone) grafted poly(acrylic acid) by NMR diffusion studies0 aInvestigation of domain structures in monomethoxy polyethylene g  cAUG  a976-9840 v713 a<p>
	Associating polymers developed by grafting a block copolymer of monomethoxy poly(ethylene glycol)-b-poly(caprolactone) (MPEG-b-PCL) onto poly(acrylic acid) undergo an irreversible sol-gel transition on heating. The influence of various physicochemical parameters on the thermoresponsive behaviour was examined by rheology and NMR studies. Pulsed field gradient NMR diffusion studies were performed to probe the mechanism of thermally induced gelation. Analysis of the diffusion data reveals the presence of loosely and strongly associated structures which respond differently to variation in temperature. It is observed that the polymer solution, which is visibly homogeneous, is heterogeneous on a mesoscopic scale with a distribution of domains. Detailed investigation of the thermally induced sol-gel transition shows that the mechanism of gelation involves irreversible alterations in the domain structure and size. (c) 2022 Society of Industrial Chemistry.</p>
10aassociating polymers10ahydrophobically modified polymers10aNMR diffusion studies10aRheology10asol-gel transition10athermoresponsive polymers1 aTiwari, Neha1 aBadiger, Manohar, Virupax1 aRajamohanan, Pattuparambil, Ramanpilla1 aRavindranathan, Sapna  uhttps://library.ncl.res.in/content/investigation-domain-structures-monomethoxy-polyethylene-glycol-b-polycaprolactone-grafted