02337nas a2200241   4500008004100000022001400041245015700055210006900212260000900281300001200290490000800302520146900310653001201779653001401791653002901805100001601834700002401850700002801874700001901902700002701921700002101948856012601969       2022                        eng d  a0141-813000aMechanistic insight into differential interactions of iron oxide nanoparticles with native, glycated albumin and their effect on erythrocytes parameters0 aMechanistic insight into differential interactions of iron oxide  cJUL   a232-2470 v2123 a<p>
	Nanoparticles and protein bioconjugates have been studied for multiple biomedical applications. We sought to investigate the interaction and structural modifications of bovine serum albumin (BSA) with iron oxide nanoparticles (IONPs). The IONPs were green synthesized using E. crassipes aqueous leaf extract following characterization using transmission electron microscopy, energy dispersive X-ray analysis and X-ray diffraction. Two different concentrations of native/glycated albumin (0.5 and 1.5 mg/ml) with IONPs were allowed to interact for 1 h at 37 degrees C. Glycation markers, protein modification markers, cellular antioxidant, and hemolysis studies showed structural modifications and conformational changes in albumin due to the presence of IONPs. UV-visible absorbance resulted in hyperchromic and bathochromic effects of IONPs-BSA conjugates. Fluorescence measurements of tyrosine, tryptophan, advanced glycated end products, and ANS binding assay were promising and quenching effects proved IONPs-BSA conjugate formation. In FTIR of BSA-IONPs, transmittance was increased in amide A and B bands while decreased in amide I and II bands. In summary, native PAGE, HPLC, and FTIR analysis displayed a differential behaviour of IONPs with native and glycated BSA. These results provided an understanding of the interaction and structural modifications of glycated and native BSA which may provide fundamental repercussions in future studies.</p>
10aAlbumin10aGlycation10airon oxide nanoparticles1 aNisar, Akib1 aAjabia, Devangi, K.1 aAgrawal, Sanskruthi, B.1 aVarma, Sanjana1 aChaudhari, Bhushan, P.1 aTupe, Rashmi, S.  uhttps://library.ncl.res.in/content/mechanistic-insight-differential-interactions-iron-oxide-nanoparticles-native-glycated