02661nas a2200241   4500008004100000022001400041245013400055210006900189260000800258300001100266490000700277520181500284653001902099653001302118653001502131653002302146653001802169100002902187700002102216700001902237700003402256856012902290       2022                        eng d  a1773-224700aBioinspired hyaluronic acid based nanofibers immobilized with 3, 4-difluorobenzylidene curcumin for treating bacterial infections0 aBioinspired hyaluronic acid based nanofibers immobilized with 3   cAUG  a1034800 v743 a<p>
	Curcumin (Cur) is a natural polyphenol with multifaceted pharmacological functions, exploited extensively for biomedical applications. Traditionally curcumin is being used as an antimicrobial agent. However, to improvise the pharmacological properties, it is being modified synthetically. One of such modified Cur is 3, 4- difluorobenzylidene curcumin (CDF) which is aimed for enhancing the anti-cancer properties. Though there are reports on the studies of anti-cancer properties involving CDF, the anti-bacterial property is yet to be demonstrated. Accordingly, in our studies, we prepared bioinspired hyaluronic acid blends immobilized with CDF and fabricated non-woven nanofiber mats. These nanofiber mats were characterized and demonstrated in vitro cell culture studies, which involved cell viability, hemolysis, anti-bacterial and cell scratch assay to understand their efficacy in treating bacteria. The molecular docking studies of CDF and Cur were performed on the dihydrofolate reductase (DHFR) enzyme receptor, which is an essential protein of S.auerus (Staphylococcus aureus). The results of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay, and hemolysis of the respective nanofiber mats with Cur and CDF showed non-toxicity and were compatible with blood cells. Further, the cell proliferation and adherence recorded &gt;60% fibroblast cells for the nanofiber mats. The anti-bacterial property of Cur and CDF was similar. The in vitro release studies for the respective Cur and CDF loaded nanofiber mats recorded a release of 25 and 37%, respectively. From these studies, we concluded that the CDF sustained its antibacterial property in addition to the improved anti-cancer property; hence CDF being synergetic, it will have a better scope in cancer therapy.</p>
10aanti-bacterial10acurcumin10ananofibers10atissue engineering10awound Healing1 aPatel, Pratikshkumar, R.1 aSingam, Amarnath1 aIyer, Arun, K.1 aGundloori, Rathna, Venkata Na  uhttps://library.ncl.res.in/content/bioinspired-hyaluronic-acid-based-nanofibers-immobilized-3-4-difluorobenzylidene-curcumin