01721nas a2200241 4500008004100000022001400041245013200055210006900187260000900256300001100265490000800276520090500284653000801189653001101197653002001208653001701228653001401245100002201259700002301281700002601304700002301330856012601353 2022 eng d a0144-861700aIsolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor0 aIsolation and structural characterization of exopolysaccharide f cJUN  a1192410 v2853 a

In the present study, a cost-effective, robust Microbioreactor based production optimization of levan like exopolysaccharide from marine Bacillus sp. SGD-03 was analysed. FE-SEM analysis has showed the significant fibrillar structure of EPS. Size exclusion chromatography and other analytical data revealed that, produced EPS has a molecular weight of 1.0 x 10(4) Da and is composed of fructose monosaccharide with hydroxyl, carbonyl, and ether groups. The backbone structure of EPS has a branching pattern of beta-(2,6) linkages which confirms the similarity with available levan like polymers. The cost-effective media composition for levan production was demonstrated. The maximum yield of crude levan obtained was 123.9 g/L by response surface methodology using robust BioLector Pro Microbioreactor, and same has been validated with shake flask, 1 L and 10 L pilot-scale fermentation.

10aEPS10amarine10aMicrobioreactor10aOptimization10astructure1 aWagh, Vasudev, S.1 aSaid, Madhukar, S.1 aBennale, Jagadish, S.1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/isolation-and-structural-characterization-exopolysaccharide-marine-bacillus-sp-and-its