01569nas a2200145 4500008004100000245015300041210006900194260000800263300001600271490000700287520095300294100002901247700002201276856012501298 2022 eng d00aPoly(N-vinylcaprolactam) containing solid lipid polymer hybrid nanoparticles for controlled delivery of a hydrophilic drug gemcitabine hydrochloride0 aPolyNvinylcaprolactam containing solid lipid polymer hybrid nano cJUN a17621-176280 v123 a

Folic acid tagged and hydrophilic polymer containing solid lipid nanoparticles (SLNs) were formulated for the controlled and targeted delivery of gemcitabine, a hydrophilic drug. Drug loaded SLNs were prepared by double emulsion method and optimized by 3(2) level factorial design. Then, a hydrophilic polymer, namely, poly(N-vinylcaprolactam) (PVCL) was incorporated in the optimized SLN batch in the first aqueous phase (W1) to obtain solid lipid polymer hybrid nanoparticles (SLPHNs) that were further decorated with folic acid (F-SLPHNs). TEM analysis of SLNs and SLPHNs revealed the spherical shape with no aggregation while SLPHNs showed higher % EE. SLPHNs exhibited limited burst release of gemcitabine compared to SLNs as well as lower overall % release. All the formulations showed good cytocompatibility against MDA-MB-231 cell lines and folic acid-tagged hybrid particles (F-SLPHNs) showed remarkably higher cellular uptake.

1 aSurapaneni, Sai, Geetika1 aAmbade, Ashootosh uhttps://library.ncl.res.in/content/polyn-vinylcaprolactam-containing-solid-lipid-polymer-hybrid-nanoparticles-controlled02223nas a2200241 4500008004100000245016000041210006900201260000800270300001100278490000800289520133700297653002101634653002301655653001801678653001601696653001701712653002301729100002901752700002601781700002601807700002601833856012201859 2023 eng d00aPermeable polymersomes from temperature and pH dual stimuli-responsive PVCL-b-PLL block copolymers for enhanced cell internalization and lysosome targeting0 aPermeable polymersomes from temperature and pH dual stimulirespo cAUG a2134540 v1513 a

A series of dual stimuli-responsive block copolymers comprising temperature-responsive poly(N-vinyl-caprolactam) (PVCL) and biodegradable pH-responsive poly(L-lysine) (PLL) of varying chain length were syn-thesized by a combination of free radical polymerization and ring opening polymerization. The block copolymers formed micelles and vesicles (polymersomes) in response to temperature and pH, respectively, in aqueous so-lution. The nanoassemblies were characterized by transmission electron microscopy and dynamic light scattering techniques. Encapsulation of both hydrophobic and hydrophilic dyes in the polymersomes was shown. Doxo-rubicin (DOX) was loaded in the polymersomes and its controlled release in response to the two stimuli, inde-pendently and jointly, was studied. The drug was found to be released due to stimuli-induced increased permeability without disassembly of the polymersomes. A significant increase in the cellular uptake of the drug-loaded polymersomes at hyperthermia conditions was demonstrated at 41 degrees C and release of the drug upon localization in lysosomes was observed. Cellular internalization pathway of the polymersomes was investigated by competitive inhibition assay and a combination of endocytic pathways dominated by caveolae-mediated mechanism was found to be operative.

10ablock copolymers10aControlled release10adrug delivery10aendocytosis10aPolymersomes10aStimuli-responsive1 aSurapaneni, Sai, Geetika1 aChoudhari, Shakeb, N.1 aAvhad, Shankarrao, V.1 aAmbade, Ashootosh, V. uhttps://library.ncl.res.in/content/permeable-polymersomes-temperature-and-ph-dual-stimuli-responsive-pvcl-b-pll-block01982nas a2200241 4500008004100000022001400041245013900055210006900194260000900263300001200272490000700284520113000291653001101421653001901432653001301451653001801464653002901482100002401511700002901535700002101564700002601585856012901611 2024 eng d a1060-132500aVisible light and pH-responsive star copolymer and doxorubicin-polymer conjugate micelles for combination drug delivery and bioimaging0 aVisible light and pHresponsive star copolymer and doxorubicinpol cFEB  a105-1160 v613 a

Amphiphilic 3-arm star copolymer containing visible light-cleavable BODIPY group and pH-responsive imine bonds at the junction of hydrophilic poly(ethylene glycol) (PEG) and two poly(epsilon-caprolactone) (PCL) arms was synthesized by the combination of ring-opening polymerization (ROP) and click chemistry. The formation and stimuli-responsive disruption of micelles were analyzed using dynamic light scattering and transmission electron microscopy. Dual stimuli-responsive release of anticancer drugs Doxorubicin (Dox) and Camptothecin (CPT) from the micelles was studied. Micellar assemblies containing Dox conjugated to PEG-BODIPY through imine bonds were prepared and controlled release of Dox in response to visible light and pH was shown. CPT and Dox-loaded star copolymer micelles as well as CPT loaded Dox-conjugated micelles were used in cellular uptake studies on MDA-MB 231 cells, and the synergistic effect of the two stimuli on the release of the two drugs was demonstrated inside the cells. Micellar assemblies also showed excellent bioimaging properties in the cellular uptake studies. [GRAPHICS]

10aBODIPY10adrug conjugate10amicelles10apH-responsive10avisible light-responsive1 aBasutkar, Nitin, B.1 aSurapaneni, Sai, Geetika1 aAlam, Md., Shafi1 aAmbade, Ashootosh, V. uhttps://library.ncl.res.in/content/visible-light-and-ph-responsive-star-copolymer-and-doxorubicin-polymer-conjugate-micelles02255nas a2200277 4500008004100000022001400041245012900055210006900184260000800253300001100261490000800272520131600280653001801596653001501614653001801629653002601647653001701673653001301690653001801703653002101721100002601742700002901768700002401797700002601821856013001847 2026 eng d a0021-899500aRedox- and pH-responsive block copolymer nanocarriers with dual drug conjugation through dynamic covalent and hydrogen bonds0 aRedox and pHresponsive block copolymer nanocarriers with dual dr cMAR ae702050 v1433 a

Loading of multiple drugs in a nanocarrier with high entrapment efficiency is important for combination therapy in cancer treatment. Here, a block copolymer comprising hydrophobic poly(epsilon-caprolactone) block with a defined number of pendent propargyl groups, polyethylene glycol monomethyl ether as a hydrophilic block, and a redox-responsive disulfide group at the block junction is synthesized using click chemistry and ring-opening polymerization (ROP). Benzaldehyde and thymine groups are introduced in the side chains for selective attachment of anti-cancer drugs, doxorubicin (DOX) and methotrexate (MTX), via the formation of pH-responsive imine linkage and hydrogen bonds, respectively. The drug-conjugated block copolymers are assembled into spherical micelles of < 200 nm, and the preferential release of DOX and MTX in response to acidic pH and redox conditions is shown. At pH 5, DOX release was 59.5%, and MTX release was 40% compared to 13% and 12% at pH 7.4, whereas at pH 5 with 10 mM GSH, a DOX release of 81.5% was observed after 48 h. Cellular uptake of drug-conjugated micelles and their apoptosis compared to free DOX in the MDA-MB-231 breast cancer cells is demonstrated. Caveolae-mediated endocytosis was found to be the major pathway used by drug-loaded nanocarriers.

10abiodegradable10acopolymers10aDOX-conjugate10adrug delivery systems10amethotrexate10amicelles10apH-responsive10aredox-responsive1 aAvhad, Shankarrao, V.1 aSurapaneni, Sai, Geetika1 aPurohit, Poorvi, M.1 aAmbade, Ashootosh, V. uhttps://library.ncl.res.in/content/redox-and-ph-responsive-block-copolymer-nanocarriers-dual-drug-conjugation-through-dynamic