01522nas a2200193   4500008004100000245010600041210006900147260000800216300001400224490000700238520084100245100002001086700002101106700001501127700001901142700001901161700002101180856012701201       2021                        eng d00aIn vitro antitubercular screening and in silico study of germacradienolide from blainvillea latifolia0 aIn vitro antitubercular screening and in silico study of germacr  cJAN  a3129-31330 v333 aBioassay-guided isolation from acetone extract of Blainvillea latifolia yielded one compound. The acetone extract, fractions and the compound 1 were investigated for antitubercular activity against Mycobacterium tuberculosis H37Ra. Compound 1 showed the activity with IC50 and MIC values at 8.9 and >100 μg/mL. However, the acetone extract of Blainvillea latifolia was inactive against two Gram negative (E. coli, P. flurescence) and two Gram-positive (S. aureus, B. subtilis) bacterial strains. Hence, it was concluded that the extract and the compound 1 are specifically active against MTB and not against bacterial strains. Molecular docking study was performed against crucial mycobacterial target MtInhA to gain an insight into the binding mode and the thermodynamic interactions governing the binding affinity of this molecule.
1 aHonmore, Varsha1 aKalyankar, Vidya1 aNatu, Arun1 aKhedkar, Vijay1 aSarkar, Dhiman1 aRojatkar, Supada  uhttps://library.ncl.res.in/content/vitro-antitubercular-screening-and-silico-study-germacradienolide-blainvillea-latifolia