01477nas a2200205   4500008004100000022001400041245011000055210006900165260011000234300001600344490000700360520061100367100003200978700002701010700003401037700001501071700003701086700002501123856012301148       2015                        eng d  a1359-734500aAssessing the effect of different shapes of glyco-gold nanoparticles on bacterial adhesion and infections0 aAssessing the effect of different shapes of glycogold nanopartic  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAUG  a15669-156720 v513 a<p>Achieving selective and sensitive carbohydrate-protein interactions (CPIs) using nanotechnology is an intriguing area of research. Here we demonstrate that the different shapes of gold nanoparticles (AuNPs) functionalized with monosaccharides tune the bacterial aggregations. The mechanism of aggregation revealed that the large number of surface interactions of rod shaped mannose-AuNPs with E. coli ORN 178 compared with spherical and star-shaped AuNPs exhibited higher avidity and sensitivity. Moreover, such sensitive binding can be used for effective inhibition of bacterial infection of cells.</p>1 aChaudhary, Preeti, Madhukar1 aSangabathuni, Sivakoti1 aMurthy, Raghavendra, Vasudeva1 aPaul, Ajay1 aThulasiram, Hirekodathakallu, V.1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/assessing-effect-different-shapes-glyco-gold-nanoparticles-bacterial-adhesion-and-001804nas a2200217   4500008004100000022001400041245010000055210006900155260011000224300001400334490000800348520093200356100001801288700002301306700002701329700002701356700003401383700001901417700002501436856012501461       2016                        eng d  a0003-265400aImmobilization of multivalent glycoprobes on gold surfaces for sensing proteins and macrophages0 aImmobilization of multivalent glycoprobes on gold surfaces for s  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcFEB  a2250-22580 v1413 a<p>The multivalent display of carbohydrates on the cell surface provides cooperative binding to improve the specific biological events. In addition to multivalency, the spatial arrangement and orientation of sugars with respect to external stimuli also trigger carbohydrate-protein interactions. Herein, we report a non-covalent host-guest strategy to immobilize heptavalent glyco-beta-cyclodextrin on gold-coated glass slides to study multivalent carbohydrate-protein interactions. We have found that the localization of sugar entities on surfaces using beta-cyclodextrin (beta-CD) chemistry increased the avidity of carbohydrate-protein and carbohydrate-macrophage interactions compared to monovalent-beta-CD sugar coated surfaces. This platform is expected to be a promising tool to amplify the avidity of sugar-mediated interactions on surfaces and contribute to the development of next generation bio-medical products.</p>1 aGade, Madhuri1 aKhandelwal, Puneet1 aSangabathuni, Sivakoti1 aBavireddi, Harikrishna1 aMurthy, Raghavendra, Vasudeva1 aPoddar, Pankaj1 aKikkeri, Raghavendra  uhttps://library.ncl.res.in/content/immobilization-multivalent-glycoprobes-gold-surfaces-sensing-proteins-and-macrophages