01931nas a2200205   4500008004100000245016400041210006900205260000800274300001000282490000700292520115900299100001701458700001801475700002501493700001701518700002001535700001901555700002401574856012701598       2020                        eng d00aSimultaneous determination of anthraquinone, flavonoids, and phenolic antidiabetic compounds from cassia auriculata seeds by validated UHPLC based MS/MS method0 aSimultaneous determination of anthraquinone flavonoids and pheno  cDEC  a82-890 v113 aA systematic isolation and characterization study for Cassia auriculata (CA) seeds resulted in identifying antidiabetic compounds 1,3,8-trihydroxyanthraquinone and quercetin, quercetin-3-O-rutinoside, gallic acid, caffeic acid, ferulic acid, and ellagic acid. The ultra-high-performance liquid chromatography based triple quadrupole mass spectrometry methodology was developed and validated for simultaneous identification and confirmation of these compounds from CA seeds. Multiple reaction monitoring (MRM) based quantification method was developed with MRM optimizer software for MS1 and MS2 mass analysis. The method was optimized on precursor ions and product ions with the ion ratio of each compound. The calibration curves of seven bioactive analytes showed excellent linearity (r2 ≥ 0.99). The quantitation results found precise (RSD, < 10 %) with good recoveries (84.58 to 101.42%). The matrix effect and extraction recoveries were found within the range (91.66 to 102.11%) for the CA seeds. This is the first MS/MS-based methodology applied to quantifying seven antidiabetic compounds in CA seeds and its extract for quality control purposes.1 aGirme, Aboli1 aSaste, Ganesh1 aChinchansure, Ashish1 aJoshi, Swati1 aKunkulol, Rahul1 aHingorani, Lal1 aPatwardhan, Bhushan  uhttps://library.ncl.res.in/content/simultaneous-determination-anthraquinone-flavonoids-and-phenolic-antidiabetic-compounds