02107nas a2200229   4500008004100000245009700041210006900138260000800207300001400215490000700229520131000236100001801546700002101564700002701585700002101612700002401633700002401657700002601681700002701707700002601734856011701760       2022                        eng d00aDeciphering the sensing of alpha-amyrin acetate with hs-DNA: a multipronged biological probe0 aDeciphering the sensing of alphaamyrin acetate with hsDNA a mult  cJAN  a1238-12430 v123 aIn this study, we focus on the biomimetic development of small molecules and their biological sensing with DNA. The binding of herring sperm deoxyribonucleic acid (hs-DNA) with naturally occurring bioactive small molecule alpha-amyrin acetate (alpha-AA), a biomimetic - isolated from the leaves of Ficus (F.) arnottiana is investigated. Collective information from various imaging, spectroscopic and biophysical experiments provides evidence that alpha-AA is a minor groove sensor of hs-DNA and preferentially binds to the A-T-rich regions. Interactions of different concentrations of small molecule alpha-AA with hsDNA were evaluated via various analytical techniques such as UV-Vis, circular dichroism (CD) and fluorescence emission spectroscopy. Fluorescence emission spectroscopy results suggest that alpha-AA decreases the emission level of hsDNA. DNA minor groove sensor Hoechst 33258 and intercalative sensor EB, melting transition analysis (T-M) and viscosity analysis clarified that alpha-AA binds to hs-DNA via a groove site. Biophysical chemistry and molecular docking studies show that hydrophobic interactions play a major role in this binding. The present research deals with a natural product biosynthesis-linked chemical-biology interface sensor as a biological probe for alpha-AA: hs-DNA.1 aPansare, Amol1 aShedge, Amol, A.1 aSonawale, Maryappa, C.1 aPansare, Shubham1 aMahakal, Akshay, D.1 aKhairkar, Shyam, R.1 aChhatre, Shraddha, Y.1 aKulal, Dnyaneshwar, K.1 aPatil, Vishwanath, R.  uhttps://library.ncl.res.in/content/deciphering-sensing-alpha-amyrin-acetate-hs-dna-multipronged-biological-probe