03000nas a2200361   4500008004100000022001400041245016800055210006900223260000800292300001100300490000700311520180600318653001902124653001602143653001202159653001902171653002402190653003002214100002002244700002002264700002702284700001602311700002002327700002102347700001902368700001802387700002002405700002102425700001702446700002202463700002602485856012702511       2021                        eng d  a2210-803300aAntimicrobial and antioxidant potential of a standardized ayurvedic formulation explains its clinical efficacy as gargles in post-radiotherapy oral cancer patients0 aAntimicrobial and antioxidant potential of a standardized ayurve  cDEC  a1005100 v303 aIntroduction: Exploring the antioxidant and antimicrobial potential of a standardized proprietary poly-herbal powder and evaluating its clinical efficacy as an Ayurvedic gargle (Gandush) for reducing oral microbial load and side effects of radiotherapy in oral cavity cancer patients was the aim of this pilot study. Methods: Formulation (Gandush Churna) comprising Terminalia chebula Retz., Terminalia bellirica (Gaertn.) Roxb., Phyllanthus emblica L. and Curcuma longa L. and its decoction (Gandush Kwath) were standardized. In-vitro antioxidant potential and antimicrobial activity against selective bacterial and fungal strains were studied. Oral cavity cancer patients who had undergone radiotherapy were enrolled. The decoction was prepared by soaking Gandush Churna in water, boiling and reducing by 50 %; and was used for gargling, twice a day, for 7 days. Total microbial count and identification of microbiota in the oral cavity as well as symptoms graded as per Common Terminology Criteria for Adverse Events, were noted before and after the treatment. Change in microbial load and shift in symptom gradations were analyzed. Results: A monograph with physicochemical, chromatography, safety and stability parameters was developed. The decoction possessed good in-vitro antioxidant and antimicrobial activities. Gargling therapy significantly reduced bacterial load while mildly controlled the fungal infection in oral cavity cancer patients. It significantly reduced the severity of symptoms viz. stomatitis and local pain, with considerably decreased xerostomia and dysphagia. Conclusions: Intervention of gargle therapy using poly-herbal formulation may serve as an effective complementary treatment to improve oral hygiene and reduce side effects of radiotherapy in oral cancer patients.10aAnti-microbial10aAntioxidant10aGandush10aGargle therapy10aOral cavity cancers10aRadiotherapy side effects1 aChavan, Sandeep1 aBhuvad, Sushama1 aKumbhlakar, Bhagyashri1 aAuti, Jyoti1 aWalunj, Tanhaji1 aPathak, Shridevi1 aTanpure, Rahul1 aGujar, Shweta1 aShinde, Jagdish1 aKulkarni, Arvind1 aGupta, Vidya1 aDeshmukh, Vineeta1 aSardeshmukh, Sadanand  uhttps://library.ncl.res.in/content/antimicrobial-and-antioxidant-potential-standardized-ayurvedic-formulation-explains-its02574nas a2200337   4500008004100000022001400041245017400055210006900229260000800298300001100306490000800317520145400325653002101779653001301800653002701813653001801840653002301858100001801881700001601899700002201915700001901937700001901956700002001975700001901995700002602014700002402040700001602064700001602080700001702096856012302113       2022                        eng d  a0925-521400aTranscriptional and translational perturbation in abiotic stress induced physiological activities and metabolic pathway networks in spongy tissue disorder of mango fruit0 aTranscriptional and translational perturbation in abiotic stress  cJUN  a1118800 v1883 a<p>
	Spongy tissue formation is economically the most detrimental but agriculturally less focused physiological disorder in mango. `Alphonso' cultivar is highly prone to oxidative stress induced spongy tissue disorder impacting biochemical and metabolic profile, thereby affecting pulp quality and nutritional value of the fruit. In the present study, comparative analysis of spongy and healthy mesocarp tissues of `Alphonso' mango by transcriptomics using Illumina sequencing and proteomics using LC-MS approaches, respectively identified and quantified many genes and proteins in the metabolic pathways responsible for the spongy tissue development. The table green and the mid ripe stages of `Alphonso' fruit ripening were evaluated by the transcriptomic study and outcomes were validated using proteomic investigations for all the four ripening stages. Colossal amount of data including 30,582 transcripts, 10,800 gene ontologies and 387 putative proteins was generated from this analysis. Current multi-omics exploration revealed the development of abiotic stress (mainly oxidative stress) induced perturbations in various metabolic pathways and their interconnections, leading to the spongy tissue formation in mango. This further unfolded the altered cell wall degradation, ethylene and flavonoid biosynthesis, fruit ripening and flavor formation, thus hampering the fruit specific characteristics in mango with spongy tissue disorder.</p>
10aOxidative stress10aProteome10aspongy tissue disorder10aTranscriptome10a` Alphonso ` mango1 aOak, Pranjali1 aJha, Vineet1 aDeshpande, Ashish1 aTanpure, Rahul1 aDawkar, Vishal1 aMundhe, Swapnil1 aGhuge, Sandeep1 aPrabhudesai, Shrikant1 aKrishanpal, Anamika1 aJere, Abhay1 aGiri, Ashok1 aGupta, Vidya  uhttps://library.ncl.res.in/content/transcriptional-and-translational-perturbation-abiotic-stress-induced-physiological