02393nas a2200205   4500008004100000020002200041245009600063210006900159260002400228300001200252520162000264100002801884700002001912700003201932700002801964700002001992700002902012700002302041856012302064       2019                        eng d  a978-981-13-8315-100aDistribution and bioprospecting potential of actinobacteria from Indian mangrove ecosystems0 aDistribution and bioprospecting potential of actinobacteria from  bSpringer, Singapore  a319-3533 aActinobacteria are the ecologically and economically important prokaryotes with the unprecedented ability to produce novel metabolites. They are widely distributed in nature due to their physiological and metabolic versatility. Actinobacteria from marine ecosystem are recognized as a promising source for novel bioactive metabolites. Notably, mangroves are the most fertile and productive marine ecosystem due to their ecophysiological conditions and vast microbial diversity. At the global level, there are many novel actinobacteria which have been reported from mangrove ecosystems and also explored for their bioprospecting potential. However, in India, microbiologists have explored actinobacteria from selected mangrove ecosystems for the past five decades with special reference to culturable diversity and antimicrobial, antimycobacterial, anticancer, and enzymatic activities. So far, around 22 rare actinobacterial genera have been reported from the Indian mangrove ecosystems. However, several other novel actinobacteria with promising bioprospecting potential are yet to be explored. Hence, research on actinobacteria in the Indian mangrove ecosystem is still in its infancy. This chapter will describe the exploratory research work carried out on the importance, diversity, antimicrobial and enzymatic activity, biodegradation, and nanotechnological potency of actinobacteria from the Indian mangrove ecosystems. This chapter will enable researchers to identify the gaps to be bridged for further research on Indian mangrove actinobacteria and exploit their potential to the maximum benefit to mankind.1 aManikkam, Radhakrishnan1 aPati, Premalata1 aThangavel, Shanmugasundaram1 aVenugopal, Gopikrishnan1 aJoseph, Jerrine1 aRamasamy, Balagurunathan1 aDastager, Syed, G.  uhttps://library.ncl.res.in/content/distribution-and-bioprospecting-potential-actinobacteria-indian-mangrove-ecosystems02733nas a2200349   4500008004100000022001400041245012900055210006900184260000800253300001400261490000800275520154800283653001901831653002001850653001301870653001801883653002601901100002801927700002201955700002801977700002202005700002202027700001902049700002702068700002202095700002902117700003802146700002302184700002702207700001802234856013102252       2023                        eng d  a0273-228900aAntibacterial and anti-HIV metabolites from marine streptomyces albus MAB56 isolated from Andaman and Nicobar Islands, India0 aAntibacterial and antiHIV metabolites from marine streptomyces a  cDEC  a7738-77540 v1953 a<p>
	Marine-derived actinobacteria have tremendous potential to produce novel metabolites with diverse biological activities. The Andaman coast of India has a lot of microbial diversity, but it is still a relatively unknown ecology for isolating novel actinobacteria with beneficial bioactive compounds. We have isolated 568 actinobacterial strains from mangrove rhizosphere sediments and sponge samples. Crude extracts from 75 distinct strains were produced by agar surface fermentation and extracted using ethyl acetate. In the disc diffusion method, 25 actinobacterial strains showed antimicrobial activity; notably, the strain MAB56 demonstrated promising broad-spectrum activity. Strain MAB56 was identified as Streptomyces albus by cultural, microscopic, and molecular methods. Conditions for bioactive metabolites from MAB56 were optimized and produced in a lab-scale fermenter. Three active metabolites (C1, C2, and C3) that showed promising broad-spectrum antimicrobial activity were isolated through HPLC-based purification. Based on the UV, FT-IR, NMR, and LC-MS analysis, the chemical nature of the active compounds was confirmed as 12-methyltetradecanoic acid (C1), palmitic acid (C2), and tridecanoic acid (C3) with molecular formulae C14H28O2, C16H32O2, and C13H26O2, respectively. Interestingly, palmitic acid (C2) also exhibited anti-HIV activity with an IC50 value of &lt; 1 mu g/ml. Our findings reveal that the actinobacteria from the Andaman marine ecosystems are promising for isolating anti-infective metabolites.</p>
10aactinobacteria10aAndaman Islands10aanti-HIV10aAntibacterial10aBioactive metabolites1 aManikkam, Radhakrishnan1 aMurthy, Sangeetha1 aPalaniappan, Sivasankar1 aKaari, Manigundan1 aSahu, Amit, Kumar1 aSaid, Madhukar1 aGanesan, Vijayalakshmi1 aKannan, Sivakumar1 aRamasamy, Balagurunathan1 aThirugnanasambandan, Somasundaram1 aDastager, Syed, G.1 aHanna, Luke, Elizabeth1 aKumar, Vanaja  uhttps://library.ncl.res.in/content/antibacterial-and-anti-hiv-metabolites-marine-streptomyces-albus-mab56-isolated-andaman-and02704nas a2200337   4500008004100000022001400041245014400055210006900199260000800268300001400276490000800290520156600298653000601864653002601870653001601896653002001912653002201932653002801954100002201982700002002004700002802024700002902052700002202081700002502103700002202128700002202150700001902172700002302191700002902214856012302243       2023                        eng d  a0273-228900aNovel finding: 2,4-Di-tert-butylphenol from streptomyces bacillaris ANS2 effective against mycobacterium tuberculosis and cancer cell lines0 aNovel finding 24Ditertbutylphenol from streptomyces bacillaris A  cNOV  a6572-65850 v1953 a<p>
	The aim of the present study is to identify actinobacteria Streptomyces bacillaris ANS2 as the source of the potentially beneficial compound 2,4-di-tert-butylphenol, describe its chemical components, and assess its anti-tubercular (TB) and anti-cancer properties. Ethyl acetate was used in the agar surface fermentation of S. bacillaris ANS2 to produce the bioactive metabolites. Using various chromatographic and spectroscopy analyses, the potential bioactive metabolite separated and identified as 2,4-di-tert-butylphenol (2,4-DTBP). The lead compound 2,4-DTBP inhibited 78% and 74% of relative light unit (RLU) decrease against MDR Mycobacterium tuberculosis at 100ug/ml and 50ug/ml concentrations, respectively. The Wayne model was used to assess the latent/dormant potential in M. tuberculosis H37RV at various doses, and the MIC for the isolated molecule was found to be 100ug/ml. Furthermore, the molecular docking of 2,4-DTBP was docked using Autodock Vinasuite onto the substrate binding site of the target Mycobacterium lysine aminotransferase (LAT) and the grid box was configured for the docking run to cover the whole LAT dimer interface. At a dosage of 1 mg/ml, the anti-cancer activity of the compound 2,4-DTBP was 88% and 89% inhibited against the HT 29 (colon cancer) and HeLa (cervical cancer) cell lines. According to our literature survey, this present finding may be the first report on anti-TB activity of 2,4-DTBP and has the potential to become an effective natural source and the promising pharmaceutical drug in the future.</p>
10a210a4-Di-tert-butylphenol10aanti-cancer10aAnti-tubercular10aMolecular docking10aStreptomyces bacillaris1 aKaari, Manigundan1 aJoseph, Jerrine1 aManikkam, Radhakrishnan1 aKalyanasundaram, Revathy1 aSivaraj, Anbarasu1 aAnbalmani, Sivarajan1 aMurthy, Sangeetha1 aSahu, Amit, Kumar1 aSaid, Madhukar1 aDastager, Syed, G.1 aRamasamy, Balagurunathan  uhttps://library.ncl.res.in/content/novel-finding-24-di-tert-butylphenol-streptomyces-bacillaris-ans2-effective-against