01864nas a2200193 4500008004100000022001400041245013100055210006900186260000800255520112600263653002201389653002101411653002901432653002301461100001701484700002401501700002301525856012201548 2023 eng d a2190-681500aPoly-gamma-glutamic acid biopolymer: a sleeping giant with diverse applications and unique opportunities for commercialization0 aPolygammaglutamic acid biopolymer a sleeping giant with diverse  cAPR3 a

Poly-gamma-glutamic acid (gamma-PGA) is a biodegradable, non-toxic, ecofriendly, and non-immunogenic biopolymer. Its phenomenal properties have gained immense attention in the field of regenerative medicine, the food industry, wastewater treatment, and even in 3D printing bio-ink. The gamma-PGA has the potential to replace synthetic non-degradable counterparts, but the main obstacle is the high production cost and lower productivity. Extensive research has been carried out to reduce the production cost by using different waste; however, it is unable to match the commercialization needs. This review focuses on the biosynthetic mechanism of gamma-PGA, its production using the synthetic medium as well as different wastes by L-glutamic acid-dependent and independent microbial strains. Furthermore, various metabolic engineering strategies and the recovery processes for gamma-PGA and their possible applications are discussed. Finally, highlights on the challenges and unique approaches to reduce the production cost and to increase the productivity for commercialization of gamma-PGA are also summarized.

10aCommercialization10aMulti-nutritious10aPoly-gamma-glutamic acid10aWaste valorization1 aNair, Pranav1 aNavale, Govinda, R.1 aDharne, Mahesh, S. uhttps://library.ncl.res.in/content/poly-gamma-glutamic-acid-biopolymer-sleeping-giant-diverse-applications-and-unique03415nas a2200193 4500008004100000245018700041210007000228260000800298300001100306490000700317520263500324100002302959700002102982700002003003700002303023700001703046700001903063856013903082 2025 eng d00aGenome mining of carbohydrate-active enzymes (CAZyme) and poly-γ-glutamic acid (γ-PGA) synthesis by Bacillus velezensis (WA11) directly from lignocellulosic biomass-based substrate0 aGenome mining of carbohydrateactive enzymes CAZyme and polyγglut cNOV a1037470 v693 a

Poly-γ-glutamic acid (γ-PGA/PGA), a non-toxic and biodegradable polymer with additional diverse properties such as higher heat resistance and water retention, is widely implicated in myriad applications, including agricultural and food processing, medical treatments, and cosmetics. The industrial bio-based production of γ-PGA is primarily hindered by the potential of microbial strains and substrate costs, which are attributed primarily to the carbon sources. The present study is a proof-of-concept study, wherein a bacterial isolate, Bacillus velezensis WA11 strain, was employed for the production of γ-PGA from sugarcane bagasse (lignocellulosic biomass) without any pretreatment or pre-processing. The CAZyme annotation identified several enzyme families involved in metabolizing complex polysaccharides, including cellulose, xylan, and lignin. We obtained 104.3 g/L γ-PGA production with a productivity of 1.09 g/L/h in the optimized synthetic medium containing maltose as a carbon source, 2.9-fold higher than the earlier study using maltose as a carbon source. Further, substituting maltose with untreated sugarcane bagasse resulted in 12 g/L of γ-PGA, higher than most of the reported studies utilizing pretreated lignocellulosic biomass (LCB) lysates for producing γ-PGA. The present study demonstrates the production of γ-PGA using lignocellulosic biomass without pretreatment, providing a chemical-free, sustainable avenue for directly utilizing untreated lignocellulosic biomass (LCB) to produce expensive polymers by employing potential bacterial strains.

1 aYadav, Rakeshkumar1 aAwathare, Pranay1 aShitut, Pushkar1 aMahajan, Vaishnavi1 aNair, Pranav1 aDharne, Mahesh uhttps://library.ncl.res.in/content/genome-mining-carbohydrate-active-enzymes-cazyme-and-poly-%CE%B3-glutamic-acid-%CE%B3-pga-synthesis