01569nas a2200253   4500008004100000022001400041245008500055210006900140260007300209300001200282490000700294520071000301653001001011653001701021653001801038653001901056653002801075100002501103700001501128700001601143700002001159700001701179856011901196       2012                        eng d  a0167-577X00aIntracellular synthesis of gold nanoparticles using alga tetraselmis kochinensis0 aIntracellular synthesis of gold nanoparticles using alga tetrase  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUL  a116-1180 v793 a<p>The development of eco-friendly synthesis protocol for the synthesis of nanoscale materials of different compositions, shapes and sizes is an important area of research in the field of nanotechnology. In this paper, we report on the use of alga Tetraselmis kochinensis in the intracellular synthesis of gold nanoparticles of dimensions 5-35 nm. The particles are more concentrated upon the cell wall than on the cytoplasmic membrane, possibly due to reduction of the metal ions by enzymes present in the cell wall and cytoplasmic membrane. These intracellular nanoparticles may have applications in drug delivery, biomedical applications, catalysis, etc. (C) 2012 Elsevier B.V. All rights reserved.</p>10aAlgae10aBiosynthesis10aNanoparticles10ananotechnology10aTetraselmis kochinensis1 aSenapati, Satyajyoti1 aSyed, Asad1 aMoeez, Sana1 aKumar, Ashutosh1 aAhmad, Absar  uhttps://library.ncl.res.in/content/intracellular-synthesis-gold-nanoparticles-using-alga-tetraselmis-kochinensis-001871nas a2200181   4500008004100000022001400041245015600055210006900211260009100280300001600371490000600387520106700393100002001460700001801480700004301498700001701541856013101558       2013                        eng d  a1932-620300aIsolation, purification and characterization of vinblastine and vincristine from endophytic fungus fusarium oxysporum isolated from catharanthus roseus0 aIsolation purification and characterization of vinblastine and v  a1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USAbPUBLIC LIBRARY SCIENCEcSEP  aUNSP e718050 v83 a<p>Endophytic fungi reside in a symbiotic fashion inside their host plants, mimic their chemistry and interestingly, produce the same natural products as their hosts and are thus being screened for the production of valuable compounds like taxol, camptothecin, podophyllotoxin, etc. Vinblastine and vincristine are excellent anti-cancer drugs but their current production using plants is non-abundant and expensive. In order to make these drugs readily available to the patients at affordable prices, we isolated the endophytic fungi from Catharanthus roseus plant and found a fungus AA-CRL-6 which produces vinblastine and vincristine in appreciable amounts. These drugs were purified by TLC and HPLC and characterized using UV-Vis spectroscopy, ESI-MS, MS/MS and H-1 NMR. One liter of culture filtrate yielded 76 mu g and 67 mu g of vinblastine and vincristine respectively. This endophytic fungal strain was identified as Fusarium oxysporum based upon its cultural and morphological characteristics and internal transcribed spacer (ITS) sequence analysis.</p>1 aKumar, Ashutosh1 aPatil, Deepak1 aRajamohanan, Pattuparambil, Ramanpilla1 aAhmad, Absar  uhttps://library.ncl.res.in/content/isolation-purification-and-characterization-vinblastine-and-vincristine-endophytic-fungus-001904nas a2200193   4500008004100000245011500041210006900156260000800225300001400233490000700247520120900254100001301463700002601476700001701502700002101519700002101540700001701561856013201578       2017                        eng d00aIdentification of biomolecules involved in the biosynthesis of gold nanoparticles from living peanut seedlings0 aIdentification of biomolecules involved in the biosynthesis of g  cMAR  a1787-17950 v173 aIn present work, we have fabricated gold nanoparticles by treating an aqueous solution of 10(-4) M HAuCl4 with the proteins extracted from roots of in vitro raised peanut seedlings and purified them by FPLC at room temperature. These proteins which tested positive for the reduction of 10(-4) M HAuCl4 were identified as Mannose glucose binding lectin and Ara h 8 allergen isoforms by ESI MS/MS. It is a well known fact that aromatic amino acids possess delocalized pi electrons in their ring structures, rendering them as the best electron donors which can transfer electrons and reduce the acceptor. These results suggest that the aromatic amino acids present in the proteins from the peanut seedlings are the primary biomolecules involved in the reduction of Au (III) to Au (0). We isolated different protein of molecular weight 16.4, 28.3 and 30 kDa involved in the formation of nanoparticles. The protein with molecular weight 16.4 kDa (allergens) yielded NPs of 25-60 nm whereas, the proteins with molecular weight 28.3 kDa and 30 kDa (lectins) yielded highly monodispersed NPs of 5-20 nm in size. The results clearly indicate that the size of nanoparticles can be controlled by different proteins.1 aRaju, D.1 aSiddiqui, Ejaz, Ahmad1 aPrasanth, S.1 aKhan, Bashir, M.1 aMehta, Urmil, J.1 aAhmad, Absar  uhttps://library.ncl.res.in/content/identification-biomolecules-involved-biosynthesis-gold-nanoparticles-living-peanut-seedlings