00799nas a2200241 4500008004100000022001400041245007100055210006900126260007700195300001200272490000600284653001100290653001700301653001500318653001200333653001800345100001700363700001700380700002600397700001500423700001400438856010500452 2005 eng d a1613-681000aExtracellular biosynthesis of bimetallic Au-Ag alloy nanoparticles0 aExtracellular biosynthesis of bimetallic AuAg alloy nanoparticle aPO BOX 10 11 61, D-69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcMAY a517-5200 v110aalloys10aBiosynthesis10acomposites10aenzymes10aNanoparticles1 aSenapati, S.1 aAhmad, Absar1 aKhan, Mohammad, Islam1 aSastry, M.1 aKumar, R. uhttps://library.ncl.res.in/content/extracellular-biosynthesis-bimetallic-au-ag-alloy-nanoparticles-002201nas a2200265 4500008004100000022001400041245010700055210006900162260009500231300001000326490000600336520126700342653001701609653001201626653001101638653000901649653002301658653001801681100001701699700002501716700002601741700001701767700001901784856013201803 2005 eng d a1550-703300aExtra-/intracellular biosynthesis of gold nanoparticles by an alkalotolerant fungus, trichothecium sp.0 aExtraintracellular biosynthesis of gold nanoparticles by an alka a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScMAR a47-530 v13 a

The development of reliable, eco-friendly processes for the synthesis of nanoscale materials is an important aspect of nanotechnology. As part of our ongoing investigation into the use of fungi for nanoparticle synthesis, we report herein that depending on the reaction conditions of the fungus Trichothecium sp. with aqueous gold ions, gold nanoparticle synthesis can be controlled to occur either within the biomass or extracellularly. Moreover, we have found that reaction of gold ions with the Trichothecium sp. fungal biomass under stationary conditions results in the rapid extracellular formation of gold nanoparticles of spherical, rod-like and triangular morphology whereas reaction of the biomass under shaking conditions resulted in intracellular growth of the nanoparticles. It is believed that on changing the reaction conditions of the fungus with gold ions, the enzymes and proteins which are being released into the medium under stationary conditions do not get released under shaking conditions resulting in the formation of extracellular or intracellular gold nanoparticles respectively. The gold nanoparticles are not toxic to the cells and the cells continued to grow after the biosynthesis of the gold nanoparticles in both the cases.

10aBiosynthesis10aenzymes10aFungus10aGold10aMorphology Control10aNanoparticles1 aAhmad, Absar1 aSenapati, Satyajyoti1 aKhan, Mohammad, Islam1 aKumar, Rajiv1 aSastry, Murali uhttps://library.ncl.res.in/content/extra-intracellular-biosynthesis-gold-nanoparticles-alkalotolerant-fungus-trichothecium-sp-002203nas a2200205 4500008004100000022001400041245009300055210006900148260007300217300001000290490000700300520147600307653001701783653001101800653001801811653001301829100001501842700001701857856012301874 2012 eng d a0927-776500aExtracellular biosynthesis of platinum nanoparticles using the fungus fusarium oxysporum0 aExtracellular biosynthesis of platinum nanoparticles using the f aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcSEP a27-310 v973 a

Nanoscience is a blooming field and promises a better future. In order to fabricate nanoparticles in an eco-friendly and inexpensive manner, significant efforts are being made to replace the chemical and physical methods currently being used with the biological methods. Chemical methods are toxic while the physical ones are very expensive. Biological methods, apart from being cost-effective, also provide protein capped nanoparticles which are thus very stable, have good dispersity and do not flocculate, and may find use in various applications. The present work emphasizes on platinum nanoparticles synthesis protocol which occurs at ambient conditions. The fungus Fusarium oxysporum when incubated with hexachloroplatinic acid (H2PtCl6) in ambient conditions reduces the precursor and leads to the formation of stable extracellular platinum nanoparticles. The biosynthesis of platinum nanoparticles was monitored by UV-visible spectroscopy and these nanoparticles were completely characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The nanoparticles are in the size range of 5-30 nm and are stabilized by proteins present in the solution. The reduction process is believed to occur enzymatically, thus creating the possibility of a rational, fungal-based method for the synthesis of nanoparticles over a wide range of chemical compositions. (C) 2012 Elsevier B.V. All rights reserved.

10aBiosynthesis10aFungus10aNanoparticles10aplatinum1 aSyed, Asad1 aAhmad, Absar uhttps://library.ncl.res.in/content/extracellular-biosynthesis-platinum-nanoparticles-using-fungus-fusarium-oxysporum-002302nas a2200241 4500008004100000022001400041245008900055210006900144260011100213300001200324490000600336520145700342653001701799653001101816653002301827653001501850653001101865653000801876100001301884700002101897700001701918856012501935 2013 eng d a1573-413700aExtra- and intracellular gold nanoparticles synthesis using live peanut callus cells0 aExtra and intracellular gold nanoparticles synthesis using live  aEXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATESbBENTHAM SCIENCE PUBL LTDcFEB a107-1120 v93 a

An important challenge of considerable topical significance in nanotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials in large quantities with variable sizes, shapes and chemical compositions. Green synthesis routes for the production of inorganic metal nanoparticles using whole cell of microorganisms and plant extracts are gaining tremendous popularity as these are non-toxic, cheap and occur at ambient conditions. The present work emphasizes on gold nanoparticles synthesis protocol using live plant callus cells. Peanut callus cells when incubated with HAuCl4 solution in ambient conditions reduced the precursor and lead to formation of well dispersed, water soluble extracellular and intracellular gold nanoparticles within 24 hours. The biosynthesis of gold nanoparticles was monitored by UV-visible spectroscopy (UV-Vis) and further characterized by X-ray diffraction analysis (XRD), Energy Dispersive Spectroscopy (EDS) and Selected Area Electron Diffraction (SAED). The particle size distribution shows that the average particle size is 50 nm for extra-and 31 nm for intracellular gold nanoparticles. The nanoparticles may be stabilized by proteins secreted by callus cells. The reduction process is believed to occur enzymatically, thus creating the possibility of a rational, plant cell-based method for the synthesis of nanoparticles over a wide range of chemical compositions.

10aBiosynthesis10acallus10agold nanoparticles10alive cells10aPeanut10aTEM1 aRaju, D.1 aMehta, Urmil, J.1 aAhmad, Absar uhttps://library.ncl.res.in/content/extra-and-intracellular-gold-nanoparticles-synthesis-using-live-peanut-callus-cells-002033nas a2200217 4500008004100000022001400041245011900055210006900174260010600243300001000349490000800359520120700367653002701574653001701601653000901618653001101627653001801638100001501656700001701671856012701688 2013 eng d a1386-142500aExtracellular biosynthesis of CdTe quantum dots by the fungus fusarium oxysporum and their anti-bacterial activity0 aExtracellular biosynthesis of CdTe quantum dots by the fungus fu aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcAPR a41-470 v1063 a

The growing demand for semiconductor [quantum dots (Q-dots)] nanoparticles has fuelled significant research in developing strategies for their synthesis and characterization. They are extensively investigated by the chemical route; on the other hand, use of microbial sources for biosynthesis witnessed the highly stable, water dispersible nanoparticles formation. Here we report, for the first time, an efficient fungal-mediated synthesis of highly fluorescent CdTe quantum dots at ambient conditions by the fungus Fusarium oxysporum when reacted with a mixture of CdCl2 and TeCl4. Characterization of these biosynthesized nanoparticles was carried out by different techniques such as Ultraviolet-visible (UV-Vis) spectroscopy, Photoluminescence (PL), X-ray Diffraction (XRD), X-ray Photoelectron spectroscopy (XPS), Transmission Electron Microscopy (TEM) and Fourier Transformed Infrared Spectroscopy (FTIR) analysis. CdTe nanoparticles shows antibacterial activity against Gram positive and Gram negative bacteria. The fungal based fabrication provides an economical, green chemistry approach for production of highly fluorescent CdTe quantum dots. (C) 2013 Elsevier B.V. All rights reserved.

10aantibacterial activity10aBiosynthesis10aCdTe10aFungus10aNanoparticles1 aSyed, Asad1 aAhmad, Absar uhttps://library.ncl.res.in/content/extracellular-biosynthesis-cdte-quantum-dots-fungus-fusarium-oxysporum-and-their-anti-001889nas a2200157 4500008004100000022001400041245015900055210006900214260011000283300001400393490000600407520115000413100002201563700001701585856012901602 2014 eng d a2046-206900aEnzyme mediated synthesis of water-dispersible, naturally protein capped, monodispersed gold nanoparticles; their characterization and mechanistic aspects0 aEnzyme mediated synthesis of waterdispersible naturally protein  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJUL a7729-77340 v43 a

Inorganic nanomaterials are conventionally synthesized under harsh environments like extremes of temperature, pressure and pH. These methods are eco-unfriendly, expensive, toxic, cumbersome, yield bigger particles which agglomerate due to not being capped by capping agents. In contrast, biological synthesis of inorganic nanomaterials occurs under ambient conditions viz. room temperature, atmospheric pressure, physiological pH and is reliable, eco-friendly and cheap. We have already reported the extracellular biosynthesis of monodispersed gold nanoparticles from the whole cells of novel extremophilic actinomycete Thermomonospora sp. In order to know the exact mechanism of synthesis, we decided to investigating it further. Here we describe the simple protocol for purification of the temperature and SDS resistant sulfite reductase enzyme and organic capping molecule, which are required for the synthesis and stabilization of gold nanoparticles respectively. This purified enzyme was then employed for the synthesis of gold nanoparticles along with the capping molecule, which render gold nanoparticles monodispersed in solution.

1 aKhan, Shadab, Ali1 aAhmad, Absar uhttps://library.ncl.res.in/content/enzyme-mediated-synthesis-water-dispersible-naturally-protein-capped-monodispersed-gold-001826nas a2200229 4500008004100000022001400041245016600055210006900221260008600290300001200376490000600388520089900394653001901293653002001312653002101332653001601353653003701369100002201406700002001428700001701448856013101465 2014 eng d a2190-428600aExtracellular biosynthesis of gadolinium oxide (Gd2O3) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol0 aExtracellular biosynthesis of gadolinium oxide Gd2O3 nanoparticl aTRAKEHNER STRASSE 7-9, FRANKFURT AM MAIN, 60487, GERMANYbBEILSTEIN-INSTITUTcMAR a249-2570 v53 a

As a part of our programme to develop nanobioconjugates for the treatment of cancer, we first synthesized extracellular, protein-capped, highly stable and well-dispersed gadolinium oxide (Gd2O3) nanoparticles by using thermophilic fungus Humicola sp. The biodistribution of the nanoparticles in rats was checked by radiolabelling with Tc-99m. Finally, these nanoparticles were bioconjugated with the chemically modified anticancer drug taxol with the aim of characterizing the role of this bioconjugate in the treatment of cancer. The biosynthesized Gd2O3 nanoparticles were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoemission spectroscopy (XPS). The Gd2O3-taxol bioconjugate was confirmed by UV-vis spectroscopy and fluorescence microscopy and was purified by using high performance liquid chromatography (HPLC).

10abioconjugation10abiodistribution10agadolinium oxide10ahumicola sp10atransmission electron microscopy1 aKhan, Shadab, Ali1 aGambhir, Sanjay1 aAhmad, Absar uhttps://library.ncl.res.in/content/extracellular-biosynthesis-gadolinium-oxide-gd2o3-nanoparticles-their-biodistribution-and-002305nas a2200277 4500008004100000022001400041245009800055210006900153260011100222300001200333490000700345520133600352653001701688653001201705653001001717653001801727653001801745100002501763700001501788700001701803700001901820700002601839700001701865700001701882856012801899 2014 eng d a1573-413700aExtracellular biosynthesis of metal sulfide nanoparticles using the fungus fusarium oxysporum0 aExtracellular biosynthesis of metal sulfide nanoparticles using  aEXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATESbBENTHAM SCIENCE PUBL LTDcJUL a588-5950 v103 a

The development of reliable, eco-friendly processes for the synthesis of nanomaterials is an important aspect of nanotechnology. One approach that shows immense potential is based on the biosynthesis of nanoparticles using microorganisms such as bacteria. In this report, we demonstrate the extracellular biosynthesis and complete characterization of metal sulfide (PbS, ZnS, MnS and NiS) nanoparticles using fungus, Fusarium oxysporum. We observed that the exposure of aqueous solution of 1 mM metal sulfate to fungus Fusarium oxysporum resulted in the formation of highly stable technologically important metal sulfide semiconductor nanoparticles. Fusarium oxysporum synthesizes lead sulfide and zinc sulfide nanoparticles of fractal type structure whereas manganese sulfide and nickel sulfide nanoparticles are of variable polydispersed morphology. The variable morphology may be due to the dissimilar interaction of metal sulfide nanoparticles with capping proteins secreted by the fungus in solution. The nanoparticles solution is found to be extremely stable with little evidence of aggregation even after a month of storage. The long term stability of the nanoparticles is due to the proteins secreted by fungus in the nanoparticle solution which bind to the surface of the nanoparticles and thus prevent aggregation.

10aBiosynthesis10aenzymes10afungi10ametal sulfide10aNanoparticles1 aSenapati, Satyajyoti1 aSyed, Asad1 aKhan, Shadab1 aPasricha, Renu1 aKhan, Mohammad, Islam1 aKumar, Rajiv1 aAhmad, Absar uhttps://library.ncl.res.in/content/extracellular-biosynthesis-metal-sulfide-nanoparticles-using-fungus-fusarium-oxysporum-002378nas a2200205 4500008004100000245013000041210006900171260000800240300001200248490000800260520161700268100002501885700002601910700002601936700002001962700001701982700002501999700002102024856012702045 2017 eng d00aEffective degradation of organic water pollutants by atmospheric non-thermal plasma torch and analysis of degradation process0 aEffective degradation of organic water pollutants by atmospheric cJAN a396-4050 v1673 aThe paper reports the use of atmospheric non-thermal plasma torch as a catalyst for degradation of various organic pollutants dissolved in water. A flow of He mixed with air was used to produce the dielectric barrier discharge (DBD), at the tip of the torch, using pulsed electric excitation at 12 kV. The torch, operated at a power of 750 mW/mm², was seen to completely degrade the aqueous solutions of the pollutants namely methylene blue (MB), methyl orange (MO) and rhodamine-B (RB), at around 10⁻⁴ M concentrations, the concentration of polluants is one order higher than of routinely used heterogeneous photocatalytic reactions, within 10 min of irradiation time at room temperature. UV Visible spectra of the organic dye molecules, monitored after different intervals of plasma-irradiation, ranging between 1 and 10 min, have been used as tools to quantify their sequential degradation. Further, instead of using He, only air was used to form plasma plume and used for degradation of organic dye which follow similar trend as that of He plasma. Further, Liquid Chromatography Mass Spectroscopy (LCMS) technique has been used to understand degradation pathway of methylene blue (MB) as a representative case. Total organic carbon (TOC) measurements indicates significant decrease in its content as a function of duration of plasma exposure onto methylene blue as a representative case. Toxicity studies were carried out onto Gram negative Escherichia coli. This indicated that methylene blue, without plasma treatment, shows growth inhibition, whereas with plasma treatment no inhibition was observed.1 aBansode, Avinash, H.1 aMore, Supriya, Eknath1 aSiddiqui, Ejaz, Ahmad1 aSatpute, Shruti1 aAhmad, Absar1 aBhoraskar, Sudha, V.1 aMathe, Vikas, L. uhttps://library.ncl.res.in/content/effective-degradation-organic-water-pollutants-atmospheric-non-thermal-plasma-torch-and02718nas a2200241 4500008004100000022001400041245011600055210006900171260000800240300001100248490000800259520187500267653002602142653001802168653001802186653002402204653002502228100002202253700002402275700003002299700001702329856013002346 2025 eng d a1387-700300aEfficient hydrogen liberation from autocatalytic wastewater treatment by green synthesized Ag2SeO3 nanocatalyst0 aEfficient hydrogen liberation from autocatalytic wastewater trea cMAY a1141060 v1753 a

Concerns about wastewater management, secure hydrogen storage, and interest in an affordable, effective, and user-friendly technique for releasing hydrogen have grown globally. Developing sustainable synthetic processes that result in industrially significant nanocatalyst to synergistically accelerate the evolution of hydrogen from wastewater treatment is extremely desirable. This work, for the first time, demonstrates the design and green fabrication of bactericidal silver selenite nanoparticles (Ag2SeO3 NPs) using the fungus Aspergillus niger for autocatalytic hydrogen production and methylene blue dye reduction by hydrolytic dehydrogenation of NaBH4. The morphology of Ag2SeO3 nanocatalysts with an average particle size of 60 nm was assessed by FETEM, while their surface chemistry, crystal structure, and optical properties were examined using XPS, XRD, and FTIR/ UV-Visible spectrophotometers, respectively. The sigmoidal trajectory of NaBH4 dehydrogenation with turnover frequencies (TOF) of 4750 mL g- 1 min- 1 suggested good autocatalytic activity of Ag2SeO3 NPs. The mechanistic study unveiled that autocatalysis was made possible by the creation of novel, active Ag co-catalyst which works synergistically with Ag2SeO3 NPs. For in-situ, real time assessment of Ag concentration during catalysis, hydrolysis of NaBH4 was carried out in the presence of methylene blue dye. The results showed that the active cocatalyst centers have a significant influence on autocatalytic hydrogen production at room temperature, reducing 98.8 % methylene blue (MB) dye in 6 min with a lesser hydrogen generation rate of 4174 mL g- 1 min- 1. The catalyst exhibited excellent stability and durability after fourth consecutive cycle, demonstrating its promise for long-term and recurring application in hydrogen liberation from autocatalytic MB dye reduction.

10aAg2SeO3 nanoparticles10aAntibacterial10aautocatalysis10aHydrogen generation10aWastewater treatment1 aIslam, Sk, Najrul1 aAnsari, Ifra, Ilyas1 aGopinath, Chinnakonda, S.1 aAhmad, Absar uhttps://library.ncl.res.in/content/efficient-hydrogen-liberation-autocatalytic-wastewater-treatment-green-synthesized-ag2seo3