01875nas a2200217   4500008004100000022001400041245013000055210006900185260009000254300001000344490000700354520103800361653002201399653001501421653002301436653001601459653001601475100002001491700001701511856012901528       2013                        eng d  a1024-242200aBiotransformation of vinblastine to vincristine by the endophytic fungus fusarium oxysporum isolated from catharanthus roseus0 aBiotransformation of vinblastine to vincristine by the endophyti  aTELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLANDbINFORMA HEALTHCAREcAPR  a89-930 v313 a<p>A fungal transformation method for the production of the less abundant but more valuable anticancer drug vincristine using the more abundant anticancer drug vinblastine by an endophytic fungus Fusarium oxysporum isolated from the plant Catharanthus roseus is described. Vinblastine dissolved in sterile water when incubated with the fungal mycelial mass was transformed into vincristine at room temperature. The biotransformation process was monitored by periodic sampling of aliquots that were extracted with ethyl acetate. The crude transformed compounds were analysed using TLC where the Rf value of the spot obtained on the 8th day was found to be similar to that of standard vincristine (Rf 0.74). Purification by HPLC using a C 18 symmetry column showed a peak with RT (34.9 min) similar to that of standard vincristine. Further characterization with the help of ESI-MS showed a peak at m/z 825 attributed to the (M + H)(+) ion. This indicated that the fungus Fusarium oxysporum had converted vinblastine to vincristine.</p>10aBiotransformation10aendophytes10afusarium oxysporum10aVinblastine10avincristine1 aKumar, Ashutosh1 aAhmad, Absar  uhttps://library.ncl.res.in/content/biotransformation-vinblastine-vincristine-endophytic-fungus-fusarium-oxysporum-isolated-001842nas a2200217   4500008004100000022001400041245008200055210006900137260006900206300001200275490000800287520110600295653001201401653002201413653001301435653001601448653000901464100002201473700001701495856011201512       2013                        eng d  a1385-894700aPhase, size and shape transformation by fungal biotransformation of bulk TiO20 aPhase size and shape transformation by fungal biotransformation   aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcAUG  a367-3710 v2303 a<p>Nanosynthesis is an ever expanding frontier in recent years in view of its implications to many future technologies covering diverse fields. Considerable progress has been made realizing high quality synthesis of elemental nanomaterials as well as compounds as chemical, biological and physical routes. The issue of technologically viable large scale synthesis still continues to be a challenge. Here we demonstrate a novel environmentally friendly top down approach to nanosynthesis which exploits the strength and peculiarities of fungus based bioleaching in extracting radicals from compounds and then providing them with a reactive as well as capping environment. Thus protein capped nanoparticles of TiO2 (5-28 nm, circular and brookite phase) are formed directly from micron size (150-250 nm, disc shape, anatase phase) powder by exposing them to a medium of fungus Humicola sp. at just 50 degrees C. The fungus Humicola sp. is not only useful for the synthesis of nanoparticles but also for the transformation of shape, phase and size of TiO2. (C) 2013 Elsevier B.V. All rights reserved.</p>10aAnatase10aBiotransformation10aBrookite10ahumicola sp10aTiO21 aKhan, Shadab, Ali1 aAhmad, Absar  uhttps://library.ncl.res.in/content/phase-size-and-shape-transformation-fungal-biotransformation-bulk-tio2-0